Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model
AUTOR(ES)
Zhou, Jiang-qiao, Qiu, Tao, Zhang, Lu, Chen, Zhong-bao, Wang, Zhi-shun, Ma, Xiao-xiong, Li, Dongyu
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2016-03
RESUMO
ABSTRACT PURPOSE: To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model. METHODS: Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8): Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis. RESULTS: Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p<0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p<0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p<0.05). CONCLUSION: Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.
Documentos Relacionados
- Effect of ischemia preconditioning on renal ischemia/reperfusion injury in rats
- Dexmedetomidine preconditioning inhibits the long term inflammation induced by renal ischemia/reperfusion injury in rats
- Parecoxib reduces renal injury in an ischemia/reperfusion model in rats
- The effects of local ischemic preconditioning and topical hypothermia in renal ischemia/reperfusion injury in rats
- Interleukin 1 pretreatment decreases ischemia/reperfusion injury.