Aderencia de Candida albicans, Candida tropicalis e Candida krusei na superficie da lingua de ratos normais e xerostomicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

The adhesion of microorganisms to host mucosal surfaces is an important prerequisite for successful microbial colonization and infection. Candida albicans is the most pathogenic of Candida species and has a high ability of adherence to epithelial cells, followed by C.tropicalis. C.krusei is the least pathogenic species, causing candidosis only in immunocompromised host. Candidal adherence is studied mainly in vitro, and there are few in vivo studies. The aim of this study was to determine in vivo C. albicans, C. tropicalis, and C.krusei adhesion to rat tongue. 270 rats were divided in 3 groups of 90 animais, each group having 45 normal and 45 xerostomic rats. Each group received oral innoculation of Candida albicans, Candida tropicalis or Candida krusei suspension and yeasts were recovered from saliva after 5, 15, 30, and 60min, and in 4, 12, 24, 48, and 72h. After saliva recovery the rats were sacrificed and their tongues removed for histological evaluation and yeasts quantification, considering the volume of saliva recovered and the number of colony-forming units (CFU). Histologically, 20 highpower fields of true papillae of the rat tongue were counted and the results showed that the Candida adherence occurred mainly over the bacterial plaque in the "soft keratin" of the papillae. Xerostomia increased the amount of bacterial plaque in the papillae and facilitated the adherence of ali three species, increasing also the number of recovered yeasts. C.krusei, C.tropicalis and C.albicans showed similar values in salivary recovery and histological quantification

ASSUNTO(S)

adesão glandulas salivares

ACESSO AO ARTIGO

Documentos Relacionados