Yeast Isolation And Purification
Mostrando 1-12 de 44 artigos, teses e dissertações.
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1. Seleção de leveduras para bioconversão de D-xilose em xilitol / Yeast selection for bioconversion of D-xylose to xilitol
Microbial species, particularly yeast, are of great importance for the production of xylitol. The xylitol production involves complicated metabolic regulation, including the transport of D-xylose, production of key enzymes and cofactor regeneration. Thus, screening of microorganisms that consume D-xylose naturally becomes a viable and effective way to obtain
Publicado em: 2010
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2. Development of a therapeutic vaccine against HPV16 / Desenvolvimento de vacina terapêutica contra HPV16
Cervical cancer is the second most common cancer among women worldwide. Most cases (83%) occur in developing countries, where they are found in relatively advanced stages and, consequently, the median survival is about 49% after five years. Therefore, an effective vaccine against HPV infections can lead to control of cancer of the cervix. Although preventabl
Publicado em: 2010
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3. Onychomycosis caused by filamentous fungi non-dermatophytes / Onicomicoses causadas por fungos filamentosos não dermatófitos
INTRODUCTION: Onychomycosis, a nail fungus infection is the most frequent nail disease, constituting about half of all nail disorder. It can be caused by dermatophytes, yeasts and non- dermatophytes filamentous fungi. OBJECTIVE: Characterize the onychomycosis caused by filamentous fungi non- dermatophytes. (1) Verify after a clinical suspicion of onychomycos
Publicado em: 2008
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4. Production, purificação, characterization and study of the alkaline application of one protease produced by 191 Cellulosimicrobium cellulans. / Produção, purificação, caracterização e estudo da aplicação de uma protease alcalina produzida por Cellulosimicrobium cellulans 191.
Cellulosimicrobium cellulans 191 is a microorganism that produces an enzyme complex capable of disrupting yeast cell walls, which is composed of b-1,3-glucanase, protease and chitinase. This microorganism adheres to yeast cell walls and than lyses them. It was isolated from industrial fermentation sludge. Dried Saccharomyces cerevisiae cells were used in the
Publicado em: 2004
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5. Isolation of the yeast histone octamer.
Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt ex
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6. Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor.
We present a new method for rapid purification to near homogeneity of sequence specific DNA binding proteins based on magnetic separation. The method is described for the purification of the yeast transcription factor tau. DNA affinity Dynabeads (monodisperse superparamagnetic particles) specifically bind the protein in the presence of competitor DNA. By mag
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7. Enzymatic Hydrolysis of Yeast Cell Walls I. Isolation of Wall-Decomposing Organisms and Separation and Purification of Lytic Enzymes
Tanaka, Hirosato (University of California, Davis), and Herman J. Phaff. Enzymatic hydrolysis of yeast cell walls. I. Isolation of wall-decomposing organisms and separation and purification of lytic enzymes. J. Bacteriol. 89:1570–1580. 1965.—A number of microorganisms, able to decompose and grow on yeast cell walls, were isolated from soil. These isolate
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8. Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone.
Conjugation in the fission yeast Schizosaccharomyces pombe is controlled by the action of mating pheromones. Here I describe the isolation and characterization of M-factor, the pheromone released by M-type cells. M-factor is a nanopeptide in which the carboxy-terminal cysteine residue is carboxy-methylated and S-alkylated, probably with a farnesyl residue: T
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9. A method for the generation of YAC transgenic mice by pronuclear microinjection.
Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has become an important goal, since it offers the potential to study the control of large and complex transcription units and identify genes by complementation. Microin
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10. The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis.
The first and committed step in synthesis of the ceramide moiety of sphingolipids is catalyzed by serine palmitoyltransferase (EC 2.3.1.50), which condenses palmitoyl-CoA and serine to form 3-ketosphinganine. This step is thought to be tightly regulated to control the synthesis of sphingolipids, but data supporting this hypothesis are lacking mainly because
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11. Isolation and Purification of Antibiotic Material from Physarum gyrosum
The myxomycete Physarum gyrosum was cultured in its plasmodial stage on agar plates containing 0.025 M phosphate buffer at pH 6.5, 2% bakers' yeast, and 0.2% glucose and was supplemented with live Escherichia coli. Extracts of these plasmodia contained several antibiotic substances. Antibiotic materials were partially purified by dialysis of the agar medium-
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12. Isolation of Skin Test-Active Preparations from Yeast-Phase Cells of Blastomyces dermatitidis
Cell wall and cytoplasmic fractions were isolated from mechanically disrupted yeast-phase cells of Blastomyces dermatitidis in an effort to obtain a reliable skin-test antigen. The biological activities of these fractions were compared with those of two blastomycin preparations. The cytoplasmic antigens exhibited a low index of specificity yet exceeded the s