Type Ii Site Specific
Mostrando 1-12 de 378 artigos, teses e dissertações.
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1. Cushing disease due to a somatic USP8 mutation in a patient with evolving pituitary hormone deficiencies due to a germline GH1 splicing variant
SUMMARY We present the unique case of an adult Brazilian woman with severe short stature due to growth hormone deficiency with a heterozygous G to T substitution in the donor splice site of intron 3 of the growth hormone 1 (GH1) gene (c.291+1G>T). In this autosomal dominant form of growth hormone deficiency (type II), exon 3 skipping results in expression of
Archives of Endocrinology and Metabolism. Publicado em: 2022
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2. Phytotoxicity of cadmium and barium and derivation of critical limits in soils / Fitotoxidade de cádmio e bário e derivação de lilites críticos em solos
Soil pollution by heavy metals caused by anthropogenic activities is a problem in many countries. Cadmium (Cd) is one of the most hazardous elements due to its relatively high mobility in soils and transfer to plants. The main human exposure pathway to Cd is through the vegetable consumption and depends on the exposure scenario. Thus, defining a critical lim
Publicado em: 2011
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3. Efetividade de intervenções na adequação da profilaxia antimicrobiana da infecção do sítio cirúrgico em um hospital universitário do Brasil
The surgical site infection is one of the main healthcare-associated infections (HAIs), the main surgical complication and the use of antimicrobials is part of a set measures used in order to reduce this infection. There are well-conducted studies with general recommendations and specific standards for each type of surgery on surgical prophylaxis with antimi
Publicado em: 2010
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4. Estoque e qualidade da matéria orgânica do solo em áreas cultivadas com café sob sistemas agroflorestal e a pleno sol / Soil organic matter stock and quality in agroforestry and full sun coffee systems
Soil organic matter (SOM) restoring is considered a main goal in the enhancement of soil quality. The evaluation of changes in the SOM as a result of soil management practices is fundamental to identify strategies to increase agricultural production, avoiding soil degradation and decreasing the emission of greenhouse gases. In Brazil, no-tillage has been bro
Publicado em: 2009
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5. Caracterização da DNase da peçonha da serpente Bothrops alternatus : comparação com a DNase acida de mamiferos envolvida em apoptose / Characterization of a Dnase from Bothrops alternatus snake venom : comparision with mamalian acid Dnases involved in apoptosis
Bothrops snake venoms are cytotoxic to a variety of cells (endothelial, smooth muscle, renal and inflammatory cells), and may cause cell death by apoptosis. The venom components implicated in apoptosis include metalloproteinases and L-amino acid oxidase. In contrast, although acidic deoxyribonucleases (DNase II) have been implicated in DNA fragmentation duri
Publicado em: 2008
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6. A low-affinity estrogen-binding site in pregnant rat uteri: analysis and partial purification.
We have identified a low-affinity (type II) estrogen-binding site (EBS) that is expressed at high levels during pregnancy in rat uteri. Although this activity was detectable in nonpregnant rat uteri, it was present in amounts (0.094 pmol/g of uteri) that were severalfold lower than the high-affinity type I estrogen receptor (0.57 pmol/g of uteri). During pre
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7. Identification of mar mutations in herpes simplex virus type 1 glycoprotein B which alter antigenic structure and function in virus penetration.
Analysis of six monoclonal antibody-resistant (mar) mutants in herpes simplex virus type 1 glycoprotein B identified two type-common (II and III) and two type-specific (I and IV) antigenic sites on this molecule. To derive additional information on the location of these sites, mar mutations were mapped and nucleotide alterations were identified by DNA sequen
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8. Genetic analysis of type-specific antigenic determinants of herpes simplex virus glycoprotein C.
Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC-1) elicits a largely serotype-specific immune response directed against previously described determinants designated antigenic sites I and II. To more precisely define these two immunodominant antigenic regions of gC-1 and to determine whether the homologous HSV-2 glycoprotein (gC-2) has similarly situat
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9. Site-specific DNA binding by the bacteriophage SP01-encoded type II DNA-binding protein.
The bacteriophage SP01 genome encodes a virus-specific type II DNA-binding protein, TF1. The bacterial proteins of this ubiquitous and evolutionarily conserved class are thought to bind non-specifically to DNA. In contrast, the experiments described here demonstrate that TF1 binds to specific sites in SP01 DNA. Several of these sites have been characterized
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10. Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure.
The human T-cell leukemia virus type II (HTLV-II) regulatory protein Rex augments cytoplasmic levels of unspliced gag-pol mRNA by acting through a Rex-responsive element (RxRE) in the long terminal repeat. Purified Rex protein binds to long terminal repeat mRNA. Here, using an immunobinding assay to measure the binding of Rex protein to mutated RxRE RNAs, we
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11. Derivatives of ColE1 cer show altered topological specificity in site-specific recombination.
ColE1 contains a 250-bp sequence (cer) which is required in cis for the conversion of plasmid dimers to monomers. Recombination between cer and parB (a dimer resolution site from plasmid CloDF13) occurs in vivo at low frequency. The properties of the resulting hybrid sites have been studied. The type I hybrid closely resembles wild-type cer. It supports intr
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12. Plasmid-encoded outer membrane protein YadA mediates specific binding of enteropathogenic yersiniae to various types of collagen.
The plasmid-encoded outer membrane protein YadA of enteropathogenic yersiniae is associated with pathogenicity. Recently, collagen binding of YadA-positive yersiniae was reported without detailed characterization (L. Emödy, J. Heesemann, H. Wolf-Watz, M. Skurnik, G. Kapperud, P. O'Toole, and T. Wadström, J. Bacteriol. 171:6674-6679, 1989). To elucidate the