Effect of Propolis on Streptococcus mutans Biofilm Formation

Abstract Objective: To evaluate the susceptibility of S. mutans during growth as a biofilm in the presence of different concentrations of propolis. Material and Methods: Three different concentrations of ethanolic extract of propolis (10%, 5%, and 2.5%) were used to evaluate its potential to attenuate the biofilm formation of S. mutans (ATCC 25175) on microplates. A crystal violet staining method was performed to measure the optical density (OD) of the biofilm biomass after 3 h and 18 h time periods. All the experiments were performed in triplicate, and the obtained data were expressed as me...