Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Abstract The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated...