Application of Response Surface Modeling and Chemometrics Methods for the Determination of Ofloxacin in Human Urine Using Dispersive Liquid-Liquid Microextraction Combined with Spectrofluorimetry

Dispersive liquid-liquid microextraction (DLLME) and spectrofluorimetry, coupled with chemometrics methods, are proposed in this work for the pre-concentration and determination of ofloxacin concentration in spiked human urine. Chloroform and acetonitrile were selected as the extraction and dispersive solvents by the one-variable-at-a-time process. The Box-Behnken design was used to optimize the other variables, including the volume of extraction and dispersion solvents, solution pH, and ionic strength. A linear calibration curve was obtained in the 5.0-120.0 ng mL-1 range under optimal condit...