11/08/2015
A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to that of chemical synthesis for producing Ba1, a cysteine-rich spider neurotoxin.
The transcription of the insecticidal neurotoxin Ba1 was obtained from a cDNA library of venom glands of the spider
