Starch Protein Isolation
Mostrando 1-12 de 20 artigos, teses e dissertações.
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1. Estudo da biodegradação da blenda poli (epsilon-caprolactona) / amido modificado/proteina isolada de soja em diferentes solos : caracterização dos produtos formados e avaliação da toxicidade
O desenvolvimento de polímeros biodegradáveis tem como objetivo contribuir com a redução do volume de lixo plástico descartado no meio ambiente. Em vista disso, a utilização de polímeros naturais na confecção de blendas tem proporcionado o aproveitamento de recursos de fontes renováveis como e o caso do amido e da soja. Nesse trabalho, dando conti
Publicado em: 2010
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2. Independent genetic control of maize starch-branching enzymes IIa and IIb. Isolation and characterization of a Sbe2a cDNA.
In maize (Zea mays L.) three isoforms of starch-branching enzyme (SBEI, SBEIIa, and SBEIIb) are involved in the synthesis of amylopectin, the branched component of starch. To isolate a cDNA encoding SBEIIa, degenerate oligonucleotides based on domains highly conserved in Sbe2 family members were used to amplify Sbe2-family cDNA from tissues lacking SBEIIb ac
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3. Isolation of a cDNA Encoding a Granule-Bound 152-Kilodalton Starch-Branching Enzyme in Wheat1
Screening of a wheat (Triticum aestivum) cDNA library for starch-branching enzyme I (SBEI) genes combined with 5′-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp composite cDNA, Sbe1c. Based on sequence alignment to characterized SBEI cDNA clones isolated from plants, the SBEIc predicted from the cDNA sequence was produced with a trans
American Society of Plant Physiologists.
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4. Differential gene expression in ripening banana fruit.
During banana (Musa acuminata L.) fruit ripening ethylene production triggers a developmental cascade that is accompanied by a massive conversion of starch to sugars, an associated burst of respiratory activity, and an increase in protein synthesis. Differential screening of cDNA libraries representing banana pulp at ripening stages 1 and 3 has led to the is
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5. Genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure.
Maltoporin (LamB protein) is a maltodextrin transport protein in the outer membrane of Escherichia coli with binding sites for bacteriophage lambda and maltosaccharides. Binding of starch by bacteria was found to inhibit swarming of Escherichia coli in soft agar plates; the inhibition was dependent on the maltodextrin affinity of maltoporin. On the basis of
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6. Starch in Fungi: III. Isolation and Properties of an Amylose-precipitating Factor from Lentinellus Ursinus Fruit Bodies 1
A factor which precipitates amylose has been isolated from Lentinellus ursinus (Fr.) Kühner fruit bodies. This factor could be a protein or a polypeptide. Glucose, maltose, and amylopectin do not affect the binding of amylose. Amylose binding is unaffected by temperature (4 to 40 C) or pH (6 to 8.5).
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7. Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.
We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in polle
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8. Isolation, by affinity chromatography, of mutant escherichia coli cells with novel regulation of lamB expression.
Affinity chromatography was used as a positive genetic selection technique for the isolation of cells exhibiting high levels of surface receptor expression. Starting from a large population of Escherichia coli with no maltodextrin receptor due to a deletion of malT, the positive regulator gene required for receptor synthesis, cells were chromatographically e
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9. Action of Bacterial Growth on the Sarcoplasmic and Urea-Soluble Proteins from Muscle: I. Effects of Clostridium perfringens, Salmonella enteritidis, Achromobacter liquefaciens, Streptococcus faecalis, and Kurthia zopfii
Comparisons of the starch-gel patterns of uninoculated aseptic control samples from rabbit and pig muscle with similar samples inoculated and incubated with Clostridium perfringens, Salmonella enteritidis, Achromobacter liquefaciens, and Kurthia zopfii were made. Results indicated that C. perfringens caused extensive alteration in the proteins or enzymes, or
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10. Lysis of erythrocytes by a hemolysin produced by a group B Streptococcus sp.
An improved procedure for the isolation and purification of the hemolysin produced by a group B streptococcus was developed, and the inactivation of partially purified hemolysin by several enzymes was studied. Hemolysin obtained in buffer containing starch and Tween 80 was inactivated by subtilisin and alpha-amylase, suggesting that the hemolysin may consist
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11. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila.
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit
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12. High-temperature production of protein-enriched feed from cassava by fungi.
A simple, nonaseptic, low-cast process for the conversion of cassava, a starchy tropical root crop, into microbial protein for use as animal feed was sought. Screening tests culminated in the isolation of a thermotolerant, amylase-producing mold, designated I-21, which was identified as Aspergillus fumigatus. The optimum pH for protein synthesis was 3-5, but