Spatial Arrangement Structure
Mostrando 13-24 de 49 artigos, teses e dissertações.
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13. The in situ spatial arrangement of the influenza A virus matrix protein M1 assessed by tritium bombardment
Intact influenza A virions were bombarded with thermally activated tritium atoms, and the intramolecular distribution of the label in the matrix protein M1 was analyzed to determine the in situ accessibility of its tryptic fragments. These data were combined with the previously reported x-ray crystal structure of the M1 fragment 2–158 [Sha, B. & Luo, M. (1
The National Academy of Sciences.
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14. Prediction of geometrically feasible three-dimensional structures of pseudoknotted RNA through free energy estimation
Accurate free energy estimation is essential for RNA structure prediction. The widely used Turner's energy model works well for nested structures. For pseudoknotted RNAs, however, there is no effective rule for estimation of loop entropy and free energy. In this work we present a new free energy estimation method, termed the pseudoknot predictor in three-dim
Cold Spring Harbor Laboratory Press.
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15. Spatial arrangement of gene products of the P1 operon in the membrane of Mycoplasma pneumoniae.
The spatial arrangement of three P1 operon-encoded proteins--attachment protein P1 (ORF5 gene product) and the ORF6-derived proteins of 40 and 90 kDa--in the membrane of Mycoplasma pneumoniae M129 was investigated by nearest-neighbor analysis. For these studies, the homobivalent, thiol-cleavable, and nonmembrane-permeating cross-linking reagent 3,3'-dithiobi
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16. Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein
Retroviral integrase, an essential enzyme for replication of human immunodeficiency virus type-1 (HIV-1) and other retroviruses, contains three structurally distinct domains, an N-terminal domain, the catalytic core and a C-terminal domain. To elucidate their spatial arrangement, we have solved the structure of a fragment of HIV-1 integrase comprising the N-
Oxford University Press.
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17. Three-dimensional crystal structure of recombinant murine interferon-beta.
The crystal structure of recombinant murine interferon-beta (IFN-beta) has been solved by the multiple isomorphous replacement method and refined to an R-factor of 20.5% against 2.6 A X-ray diffraction data. The structure shows a variant of the alpha-helix bundle with a new chain-folding topology, which seems to represent a basic structural framework of all
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18. Structural Model for 12-Helix Transporters Belonging to the Major Facilitator Superfamily
The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 Å resolution, of the oxalate transporter, OxlT
American Society for Microbiology.
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19. In Situ Spatial Organization of Potato Virus A Coat Protein Subunits as Assessed by Tritium Bombardment
Potato virus A (PVA) particles were bombarded with thermally activated tritium atoms, and the intramolecular distribution of the label in the amino acids of the coat protein was determined to assess their in situ steric accessibility. This method revealed that the N-terminal 15 amino acids of the PVA coat protein and a region comprising amino acids 27 to 50
American Society for Microbiology.
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20. Monoclonal antibody R2D5 reveals midsagittal radial glial system in postnatally developing and adult brainstem.
Radial glial cells and their processes play critical roles in organizing the spatial arrangement of the nervous system in the embryonic brain. It has been thought that following completion of their roles in the embryo, most of the radial glial processes disappear before or shortly after birth. Here we use R2D5, a monoclonal antibody to a soluble cytosolic pr
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21. The spatial configuration of ordered polynucleotide chains. II. The poly(rA) helix.
Approximate details of the spatial configuration of the ordered single-stranded poly(rA) molecule in dilute solution have been obtained in a combined theoretical analysis of base stacking and chain flexibility. Only those regularly repeating structures which fulfill the criterion of conformational flexibility (based upon all available experimental and theore
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22. The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation
The initiation of DNA replication is a key event in the cell cycle of all organisms. In bacteria, replication initiation occurs at specific origin sequences that are recognized and processed by an oligomeric complex of the initiator protein DnaA. We have determined the structure of the conserved core of the Aquifex aeolicus DnaA protein to 2.7 Å resolutio
Oxford University Press.
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23. Surface structure of the COPII-coated vesicle
The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and by electron microscopy of coat proteins and coated vesicle surfaces. The efficiency of COPII subunit crosslinking to phospholipids declined in order of protein recruitment to the coat: Sar1p > Sec23/24p ≫ Sec13/31p. Deep-etch rotary sha
The National Academy of Sciences.
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24. Solution structure of GAP SH3 domain by 1H NMR and spatial arrangement of essential Ras signaling-involved sequence.
Src homology 3 (SH3) domains are found in numerous cytoplasmic proteins involved in intracellular signal transduction. We used 2-D 1H NMR to determine the structure of the SH3 domain of the guanosine triphosphatase-activating protein (GAP), an essential component of the Ras signaling pathway. The structure of the GAP SH3 domain (275-350) was found to be a co