Sequence Tagged Sites
Mostrando 1-12 de 110 artigos, teses e dissertações.
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1. Avaliação de pacientes com hanseníase na faixa virchowiana diagnosticados entre 1990 e 2000 e tratados com poliquimioterapia 24 doses e seus comunicantes na fase pós-eliminação em municípios de Santa Catarina / Assessment of lepromatous leprosy patients diagnosed among 1990 and 2000 and treated with multidrugtherapy 24 doses and their household contacts in the post-elimination phase in Santa Catarina municipalities
INTRODUÇÃO: A poliquimioterapia (PQT-OMS) para tratamento da hanseníase resultou em drástica redução da sua prevalência, mas em limitado impacto na detecção de casos novos (CN), que se manteve estável em Santa Catarina, estado na fase de pós-eliminação. Casos com altos índices baciloscópicos apresentam risco de recidiva tardia e podem consisti
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/08/2011
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2. Obtenção de SNPs, reação do feijoeiro comum à ferrugem da soja e piramidação de genes de resistência a Uromyces appendiculatus / SNP discovery, reaction of the common bean to soybean rust, and pyramiding of resistance genes to Uromyces appendiculatus
Este trabalho apresenta resultados de pesquisas desenvolvidas no âmbito do Programa de Melhoramento do Feijoeiro conduzido na Universidade Federal de Viçosa (UFV), sendo este composto por seções ou subprojetos distintos. Desta forma, optou-se por redigi-lo na forma de artigos científicos, sendo um de revisão e quatro de resultados originais, os quais j
Publicado em: 2009
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3. Somatic cytogenetic and azoospermia factor gene microdeletion studies in infertile men
The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural select
Brazilian Journal of Medical and Biological Research. Publicado em: 2006-04
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4. Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men
We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified effici
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-06
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5. Conserved sequence-tagged sites: a phylogenetic approach to genome mapping.
Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences. The great majority of such syntenically equivalent sequence-tagged sites (STSs) from human DNA can be used to assemble and format corresponding maps for other primates, and some based on gene sequences are show
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6. Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markers for gene mapping
We present an approach to map large numbers of Tc1 transposon insertions in the genome of Caenorhabditis elegans. Strains have been described that contain up to 500 polymorphic Tc1 insertions. From these we have cloned and shotgun sequenced over 2000 Tc1 flanks, resulting in an estimated set of 400 or more distinct Tc1 insertion alleles. Alignment of th
The National Academy of Sciences of the USA.
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7. Direct cloning of sequence tagged microsatellite sites by DNA affinity chromatography.
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8. Two sequence-tagged sites defining the ends of a 380 kb YAC clone from 19q13.
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9. Duplication of U3 Sequences in the Long Terminal Repeat of Mink Cell Focus-Inducing Viruses Generates Redundancies of Transcription Factor Binding Sites Important for the Induction of Thymomas
The ability of mink cell focus-inducing (MCF) viruses to induce thymomas is determined, in part, by transcriptional enhancers in the U3 region of their long terminal repeats (LTRs). To elucidate sequence motifs important for enhancer function in vivo, we injected newborn mice with MCF 1dr (supF), a weakly pathogenic, molecularly tagged (supF) MCF virus conta
American Society for Microbiology.
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10. Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing
We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5′-ends. Amplification products are then combined and converted to a concata
Oxford University Press.
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11. Bundling of microtubules in transfected cells does not involve an autonomous dimerization site on the MAP2 molecule.
We have searched for putative dimerization sites in microtubule-associated protein 2 (MAP2) that may be involved in the bundling of microtubules. An overlapping series of fragments of the embryonic form MAP2c were created and immunologically "tagged" with an 11 amino acid sequence from human c-myc. Nonneuronal cells were transfected simultaneously with one o
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12. A Physical Map of the X Chromosome of Drosophila Melanogaster: Cosmid Contigs and Sequence Tagged Sites
A physical map of the euchromatic X chromosome of Drosophila melanogaster has been constructed by assembling contiguous arrays of cosmids that were selected by screening a library with DNA isolated from microamplified chromosomal divisions. This map, consisting of 893 cosmids, covers ~64% of the euchromatic part of the chromosome. In addition, 568 sequence t