Rna Polymerases
Mostrando 1-12 de 769 artigos, teses e dissertações.
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1. Caracterização de cepas de Escherichia coli contendo diferentes alelos rpoS. / Characterization of Escherichia coli strains carrying different rpos alleles.
Escherichia coli can be found in many different habitats and has to be prepared to survive and grow under unfavorable conditions. Bacteria adaptation to different growth conditions requires an efficient control of gene expression. One of the primary forms of gene expression control is the competition between different sigma factors for the binding to the cor
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/08/2011
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2. Identificação e análise de expressão de RNAs intrônicos não codificadores humanos / Identification and expression analysis of human intronic noncoding RNAs
In this work, we show large-scale studies of antisense noncoding RNAs transcribed from intronic regions of human genes. The correlation of expression levels of some intronic transcripts to the degree of tumor differentiation in prostate cancer points to the biological relevance of these messages in complex diseases such as cancer. We also evaluated the exist
Publicado em: 2007
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3. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases
BioMed Central.
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4. Archaebacteria and eukaryotes possess DNA-dependent RNA polymerases of a common type.
DNA-dependent RNA polymerases of archaebacteria not only resemble the nuclear RNA polymerases of eukaryotes rather than the eubacterial enzymes in their complex component patterns but also show striking immunochemical, i.e., structural, homology with the eukaryotic polymerases at the level of single components. Thus, eukaryotic and archaebacterial RNA polyme
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5. Characterization of the RNA polymerases of Trypanosoma brucei: trypanosomal mRNAs are composed of transcripts derived from both RNA polymerase II and III.
To analyze transcription in Typanosoma brucei, we have characterized the trypanosomal RNA polymerases. Here we present our results, which allow a discrimination between the different classes of RNA polymerases in nuclear run-on experiments by polymerase inhibitors and Mn2+ dependence. We also describe the separation of trypanosomal RNA polymerases by chromat
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6. Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits.
We have determined the nucleotide sequence of a region of the vaccinia virus genome encoding RNA polymerase subunits of 22 and 147 kDa and have mapped the 5' and 3' ends of the two mRNAs. The predicted amino acid sequence of the vaccinia 147-kDa subunit shows extensive homology with the largest subunit of Escherichia coli RNA polymerase, yeast RNA polymerase
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7. Products of RNA Polymerases in HeLa Cell Nuclei
RNA polymerase activities in intact HeLa cell nuclei have been examined and compared to activities investigated in previous studies of the purified enzymes. The RNA synthesized by the mammalian polymerases while still in nuclei is identified. The polymerases are tentatively identified by location, sensitivity to α-amanitin, and response to manganese and alt
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8. A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells
Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA
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9. Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.
Three peaks of DNA-dependent RNA polymerase (RNA nucleotidyltransferase) activity are resolved by chromatography of a sonicated yeast cell extract on DEAE-Sephadex. The enzymes, which are named RNA polymerases I, II, and III in order of elution, show similar catalytic properties to the vertebrate class I, class II, and class III RNA polymerases, respectively
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10. Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif.
RNA-dependent RNA polymerases contain a highly conserved region of amino acids with a core segment composed of the amino acids YGDD which have been hypothesized to be at or near the catalytic active site of the molecule. Six mutations in this conserved YGDD region of the poliovirus RNA-dependent RNA polymerase were made by using oligonucleotide site-directed
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11. Effects of abasic sites and DNA single-strand breaks on prokaryotic RNA polymerases.
Abasic sites are thought to be the most frequently occurring cellular DNA damage and are generated spontaneously or as the result of chemical or radiation damage to DNA. In contrast to the wealth of information that exists on the effects of abasic sites on DNA polymerases, very little is known about how these lesions interact with RNA polymerases. An in vitr
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12. Autoantibody reactive with three classes of RNA polymerases in sera from patients with systemic sclerosis.
We have identified a novel autoantibody reactive with all three classes of RNA polymerases, well-characterized nuclear enzymes, in sera from patients with systemic sclerosis (SSc). After incubation with [35S]methionine-labeled HeLa cell extracts, 14 of 275 SSc sera immunoprecipitated 12 or 14 proteins with similar molecular weights as those of several subuni