Ribonucleoproteins
Mostrando 1-12 de 249 artigos, teses e dissertações.
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1. New insights into trypanosomatid U5 small nuclear ribonucleoproteins
Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certain small complexes of RNA and protein, whi
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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2. Functional characterization os mRNA-protein complexes (mRNPs) in Trypanosoma cruzi. / Caracterização funcional de complexos mRNA-proteínas (mRNPs) em Trypanosoma cruzi.
Em tripanossomatídeos a regulação da expressão gênica ocorre principalmente em nível pós-transcricional. Acredita-se que a estabilidade do mRNA e o acesso aos polissomos sejam fortemente regulados, permitindo ao Trypanosoma cruzi uma rápida adaptação à diferentes condições ambientais as quais está exposto durante seu ciclo de vida. A regulaçã
Publicado em: 2010
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3. Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1 / Funcional and structural studies of human protein hnRNPQ
The members of the hnRNPs family (heterogenous nuclear ribonuclein proteins) play important roles in gene expression control and mRNAs metabolism. The proteins hnRNPD (AUF1) and hnRNPQ (NSAP1) were the main targets of this study. AUF1 has two RNA recognition motifs (RRM) and participates in the process of destabilization of a class of mRNAs that contain AU-r
Publicado em: 2008
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4. Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing mi
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-07
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5. Presence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patients
We detected anti-human small nuclear ribonucleoprotein (snRNP) autoantibodies in chagasic patients by different immunological methods using HeLa snRNPs. ELISA with Trypanosoma cruzi total lysate antigen or HeLa human U small nuclear ribonucleoproteins (UsnRNPs) followed by incubation with sera from chronic chagasic and non-chagasic cardiac patients was used
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-01
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6. Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins.
Ro small ribonucleoproteins consist of a 60-kDa protein and possibly additional proteins complexed with several small RNA molecules. The RNA components of these particles, designated Y RNAs, are about 100 nt long. Although these small ribonucleoproteins are abundant components of a variety of vertebrate species and cell types, their subcellular location is c
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7. Domains required for nucleic acid binding activities in chloroplast ribonucleoproteins.
Five ribonucleoproteins (or RNA-binding proteins) from tobacco chloroplasts have been identified to date; each of these contains an acidic N-terminal domain (24-64 amino acids) and two conserved RNA-binding domains (82-83 amino acids). All five ribonucleoproteins can bind to ssDNA and dsDNA but show high specificity for poly(G) and poly(U). Here we present t
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8. Release of in vitro-synthesized poly(A)-containing RNA from isolated rat liver nuclei: Characterization of the ribonucleoprotein particles involved
Nuclei isolated from rat liver were incubated under conditions in which RNA continued to be labeled with precursors for long periods. After 1 hr, during which the rate of RNA synthesis was constant, 25-30% of the newly synthesized RNA was recovered in the postnuclear supernatant. About 3-5% of this fraction was characterized as poly(A)-containing ribonucleop
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9. Borna disease virus (BDV), a nonsegmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present.
Borna disease virus (BDV) causes neurological disease in a wide range of animal species, providing an important model for studies of persistent viral infection of the central nervous system. In addition, the detection of antibodies that react with BDV antigen in serum from psychiatric patients suggests a role for BDV, or related viruses, in human mental diso
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10. Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
Pre-mRNA substrates containing sequences from human and mouse histone genes are accurately processed in a HeLa cell nuclear extract to generate mature 3' termini. When in vitro processing reactions containing either human histone H3 or mouse histone H3 transcripts are treated with RNase T1 and probed with antibodies specific for the Sm protein determinants o
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11. Nucleic acid-binding specificities of tobacco chloroplast ribonucleoproteins
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12. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
Small ribonucleic acid (RNA)-protein complexes precipitated by anti-Ro and anti-La antibodies from lupus patients have been examined with emphasis on their RNA components. In both ribonucleoprotein (RNP) classes, the numbers of different RNA molecules and their sequences vary between mouse and human cells. The complex mixtures of La RNAs include two previous