Recombinant Protein Purification
Mostrando 1-12 de 395 artigos, teses e dissertações.
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1. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I
Braz. J. Microbiol.. Publicado em: 2018-06
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2. Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli
ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aim
Braz. arch. biol. technol.. Publicado em: 07/08/2017
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3. Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli
Human enterokinase (synonym: enteropeptidase, EC 3.4.21.9) light chain (hEKL) gene was designed and artificially synthesized with built-in codon blas towards Escherichia colicodon preference. The synthetic hEKL gene was cloned into prokaryotic expression vector pMAL-s and transferred into the expression strain E. coli BL21 (DE3). Recombinant hEKL protein wit
Braz. arch. biol. technol.. Publicado em: 14/10/2014
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4. Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)
A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E. coli when the strains were grown for 4-5 h at 37 ºC. Highly pure protein was isolated by Ni2+-resin chromatography with 150 mM
Braz. arch. biol. technol.. Publicado em: 2013-08
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5. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis
The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)]3 and 4-MU-(GlcNAc)2. Two proteins, 45 kDa and 39 kDa in si
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-05
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6. Expressão heteróloga de neurotoxinas do veneno da aranha Phoneutria nigriventer
The venom of the spider Phoneutria nigriventer contains several peptides that act on ion channels and receptors of the nervous system of insects and mammals. The toxins Tx3-6 and Tx3-4 are voltage-gated calcium channel blockers and have shown a pharmacological potential in the treatment of pain and in neuroprotection. Only small amounts of these toxins can b
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/06/2011
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7. ExpressÃo de uma quitinase de Chromobacterium Violaceum em Pichia Pastoris: purificaÃÃo e caracterizaÃÃo parcial da proteÃna recombinante / Expression of a chitinase from Chromobacterium Violaceum in Pichia pastoris: purification and partial characterization of recombinant protein
A utilizaÃÃo de microrganismos como sistemas heterÃlogos de expressÃo de proteÃnas tem se mostrado uma estratÃgia alternativa e/ou complementar aos passos tradicionais utilizados no processo de purificaÃÃo de proteÃnas. Diante deste contexto, quitinases (EC 3.2.1.14), enzimas hidrolÃticas capazes de degradar quitina, tÃm sido expressas em diferent
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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8. Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion
The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring green fluorescent prote
Genetics and Molecular Biology. Publicado em: 16/09/2011
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9. Expression and purification of the non-tagged LipL32 of pathogenic Leptospira
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternat
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-04
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10. Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em Escherichia coli. / Expression of recombinant human colony stimulating factor (rhG-CSF) in Escherichia coli.
The recombinant human granulocyte colony stimulating factor (rhG-CSF) is a non-glycosylated protein with 175 amino acids. This factor plays an important role in hematopoietic cell proliferation and has been widely used for treating neutropenia. The purpose of this work is to construct two expression systems in E. coli; a system for obtaining rhG-CSF in the c
Publicado em: 2010
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11. Produção de alérgeno Blo t 5 recombinante (rBlo t 5) de Blomia tropicalis e reatividade de anticorpos IgE a alérgenos Blo t 5 nativo e recombinante em pacientes atópicos
Allergies are caused by immunological reactions to allergens, which are substances capable of triggering specific IgE responses in genetically predisposed subjects. In tropical and subtropical regions, allergens derived from B. tropicalis are important causes of IgE-mediated sensitization among patients with asthma and allergic rhinitis. The aim of this stud
Publicado em: 2010
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12. Produção e caracterização de leptina e TcPTP1 recombinantes, proteínas ligadas ao metabolismo, sinalização celular e inflamação. / Production and characterization of recombinant leptin and TcPTP1, proteins linked to metabolism, cell sinalization and inflamation.
Leptin is predominantly secreted by adipocytes and plays an important role in the regulation of the energetic metabolism of mammals. Because of this, leptin is strongly involved in the regulation of obesity. After ligation, dimerization and activation of its specific receptor in the hypothalamus, leptin activates JAK kinases that phosphorylate STAT transcrip
Publicado em: 2010