Recombinant E Coli
Mostrando 13-24 de 1801 artigos, teses e dissertações.
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13. Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors
Hepatitis C virus (HCV) envelope protein 2 (E2) is involved in viral binding to host cells. The aim of this work was to produce recombinant E2B and E2Y HCV proteins in Escherichia coli and Pichia pastoris, respectively, and to study their interactions with low-density lipoprotein receptor (LDLr) and CD81 in human umbilical vein endothelial cells (HUVEC) and
Mem. Inst. Oswaldo Cruz. Publicado em: 26/05/2015
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14. Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli
Human enterokinase (synonym: enteropeptidase, EC 3.4.21.9) light chain (hEKL) gene was designed and artificially synthesized with built-in codon blas towards Escherichia colicodon preference. The synthetic hEKL gene was cloned into prokaryotic expression vector pMAL-s and transferred into the expression strain E. coli BL21 (DE3). Recombinant hEKL protein wit
Braz. arch. biol. technol.. Publicado em: 14/10/2014
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15. Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure
The hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant of fibrosis progression and, consequently, cirrhosis. The a
Mem. Inst. Oswaldo Cruz. Publicado em: 09/09/2014
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16. A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for a
Braz J Infect Dis. Publicado em: 2014-06
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17. A supervision and control tool based on artificial intelligence for high cell density cultivations
High cell density cultivations of recombinant E. coli have been increasingly used for the production of heterologous proteins. However, it is a challenge to maintain these cultivations within the desired conditions, given that some variables such as dissolved oxygen concentration (DOC) and feed flow rate are difficult to control. This paper describes the sof
Braz. J. Chem. Eng.. Publicado em: 2014-06
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18. Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent
Braz. J. Microbiol.. Publicado em: 04/03/2014
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19. Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pasto
Braz. arch. biol. technol.. Publicado em: 31/01/2014
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20. Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)
A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E. coli when the strains were grown for 4-5 h at 37 ºC. Highly pure protein was isolated by Ni2+-resin chromatography with 150 mM
Braz. arch. biol. technol.. Publicado em: 2013-08
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21. Recombinant Erwinia carotovora l-asparaginase II production in Escherichia coli fed-batch cultures
Asparaginases are the cornerstone therapy of many successful combination regimens for the treatment of acute lymphoblastic leukemia (ALL), the most common malignancy in children and adolescents. The aim of this work was to produce recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures. Using a robust fed-batch technique with
Braz. J. Chem. Eng.. Publicado em: 2013-06
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22. Avaliação da temperatura de indução e de fontes de nitrogênio na produção de proteína de superfície de Streptococcus pneumoniae em Escherichia coli recombinante
Doenças causadas por Streptococcus pneumoniae constituem um dos principais problemas de saúde pública mundial. A proteína A de superfície de pneumococo (PspA) é candidata em potencial a ser carreadora em vacina conjugada contra essa bactéria. Considerando as altas perdas inerentes às etapas de purificação e conjugação da proteína, é fundamental
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/08/2012
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23. Production and characterization of recombinant chimeras C and D of Clostridium botulinum / Produção e caracterização de quimeras recombinantes C e D de Clostridium botulinum
Bovine Botulism is a lethal intoxication caused by the ingestion of the neurotoxins produced by Clostridium botulinum types C and D that inhibit the release of acetylcholine at the neuromuscular junction leading to death by flaccid paralysis. It produces important economic losses, being a major cause of casualties in cattle in several regions of Brazil. The
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/08/2012
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24. Produção e caracterização de quimeras recombinantes C e D de Clostridium botulinum / Production and characterization of recombinant chimeras C and D of Clostridium botulinum
O botulismo bovino é uma intoxicação letal causada pela ingestão da neurotoxina produzida pelo Clostridium botulinum principalmente dos tipos C e D que atua inibindo a liberação de acetilcolina na junção neuromuscular levando à morte por paralisia flácida, com grande importância econômica e sanitária, sendo uma das principais causas de morte em
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/08/2012