Pichia Pastoris Heterologous Expression
Mostrando 13-24 de 25 artigos, teses e dissertações.
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13. ExpressÃo de uma proteÃna YD de Chromobacterium violaceum em sistemas heterÃlogos: potencial no controle de pragas e fungos / Expression of a Chromobacterium violaceum YD protein in heterologous systems: potential on pests and fungi control
Chromobacterium violaceum à uma betaproteobactÃria de vida livre encontrada em regiÃes tropicais e subtropicais. C. subtsugae, bactÃria do mesmo gÃnero, possui atividade tÃxica contra insetos de diversos gÃneros. Entre os genes que podem estar envolvidos no potencial inseticida dessa bactÃria, destacam-se aqueles que codificam quitinases. Em adiÃÃo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 25/09/2008
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14. Expressão heteróloga do gene Hxyn2 do fungo Humicola grisea var. thermoidea em Pichia pastoris: Produção e purificação da enzima HXYN2r e aplicação em testes de panificação / Heterologous expression of the gene Hxyn2 fungus Humicola grisea var. thermoidea in Pichia pastoris: production and purification of the enzyme HXYN2r testing and application in bakery
As endoxilanases formam o principal grupo de enzimas hidrolíticas envolvidas na degradação da xilana. Estas enzimas têm vasta aplicação no setor industrial, como nas indústrias de bebidas, alimentícia, têxtil, de rações e de celulose (biobranqueamento). As xilanases têm sido empregadas na panificação para melhorar o processamento e a qualidade
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/05/2008
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15. Utilização do promotor do gene PGK1 de Pichia pastoris para expressão heteróloga
The methylotrophic yeast Pichia pastoris has been successfully used for the expression of heterologous proteins and several different expression vectors have been developed for this system. The main expression vectors for P. pastoris are based on the alcohol oxidase 1 gene promoter, AOX1. Some studies have been carried out aiming at the use of alternative pr
Publicado em: 2008
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16. Expressão heteróloga do gene Hxyn2 do fungo Humicola grisea var. thermoidea em Pichia pastoris: Produção e purificação da enzima HXYN2r e aplicação em testes de panificação / Heterologous expression of the gene Hxyn2 fungus Humicola grisea var. thermoidea in Pichia pastoris: production and purification of the enzyme HXYN2r testing and application in bakery
Endoxylanases are the main group of enzymes involved in the hydrolysis of xylan. This enzymes have application in industrial proposes, like as drink, food, feed, clothes industries and for bleaching cellulose paper pulps. In bread making the xylanases have been used to improve processing and product quality of loaf, leading soft dough and loaf with larger vo
Publicado em: 2008
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17. High expression of human carboxypeptidase M in Pichia pastoris: Purification and partial characterization
Carboxypeptidase M (CPM) is an extracellular glycosylphosphatidyl-inositol-anchored membrane glycoprotein, which removes the C-terminal basic residues, lysine and arginine, from peptides and proteins at neutral pH. CPM plays an important role in the control of peptide hormones and growth factor activity on the cell surface. The present study was carried out
Brazilian Journal of Medical and Biological Research. Publicado em: 2006-02
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18. Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation
BioMed Central.
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19. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris.
Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase (AOX) and dihydroxy-acetone synthase (DAS) genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for the expression of the Escherichia coli lacZ gene. These vectors were transformed into P. pastoris host cells and empl
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20. Effect of Codon Optimization on Expression Levels of a Functionally Folded Malaria Vaccine Candidate in Prokaryotic and Eukaryotic Expression Systems
We have produced two synthetic genes that code for the F2 domain located within region II of the 175-kDa Plasmodium falciparum erythrocyte binding antigen (EBA-175) to determine the effects of codon alteration on protein expression in homologous and heterologous host systems. EBA-175 plays a key role in the process of merozoite invasion into erythrocytes thr
American Society for Microbiology.
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21. High-Level Expression of the Malaria Blood-Stage Vaccine Candidate Plasmodium falciparum Apical Membrane Antigen 1 and Induction of Antibodies That Inhibit Erythrocyte Invasion
Apical membrane antigen 1 (AMA-1) is a highly promising malaria blood-stage vaccine candidate that has induced protection in rodent and nonhuman primate models of malaria. Authentic conformation of the protein appears to be essential for the induction of parasite-inhibitory antibody responses. Here we have developed a synthetic gene with adapted codon usage
American Society for Microbiology.
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22. Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase.
Nitrate reductase (NR) is rapidly inactivated by phosphorylation of serine residues in response to loss of light or reduction in CO2 levels. To identify sites within NR protein that play a role in this post-translational regulation, a heterologous expression system and an in vitro inactivation assay for Arabidopsis NR were developed. Protein extracts contain
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23. Characterization of a Tomato Xyloglucan Endotransglycosylase Gene That Is Down-Regulated by Auxin in Etiolated Hypocotyls1
The reorganization of the cellulose-xyloglucan matrix is proposed to serve as an important mechanism in the control of strength and extensibility of the plant primary cell wall. One of the key enzymes associated with xyloglucan metabolism is xyloglucan endotransglycosylase (XET), which catalyzes the endocleavage and religation of xyloglucan molecules. As wit
American Society of Plant Physiologists.
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24. Characterization and Heterologous Gene Expression of a Novel Esterase from Lactobacillus casei CL96
A novel esterase gene (estI) of Lactobacillus casei CL96 was localized on a 3.3-kb BamHI DNA fragment containing an open reading frame (ORF) of 1,800 bp. The ORF of estI was isolated by PCR and expressed in Escherichia coli, the methylotrophic bacterium Methylobacterium extorquens, and the methylotrophic yeast Pichia pastoris under the control of T7, methano
American Society for Microbiology.