Phage Display In Vivo
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Seleção in vivo de peptídeos ligantes à articulações inflamadas e seu potencial uso em diagnóstico
CAPÍTULO I: O sistema imunológico é constituído de uma complexa rede de moléculas e células que funciona a fim de proteger o organismo contra patógenos. O sistema imune sadio deve manter o balanço entre a capacidade de responder a agentes infecciosos e de sustentar a autotolerância. No entanto, podem ocorrer falhas que resultam em reações contra a
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/07/2012
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2. Caracterização de marcadores de virulência em Paracoccidioides brasiliensis. / Characterization of markers of virulence in Paracoccidioides brasiliensis.
Paracoccidioidomycosis (PCM) is a human systemic granulomatous disease, prevalent in South America, caused by a thermodimorphic fungus, Paracoccidioides brasiliensis. This fungus presents complex antigenic structure and some of these components have been related with its pathogenicity, of which little is known. The virulence recovery of isolates by passage i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/06/2009
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3. Expressão de genes das vias de biossíntese e degradação do hormônio juvenil e caracterização de peptídeos ligantes ao cérebro de Melipona scutellaris (Hymenoptera, Apidae, Meliponini)
Abelhas do gênero Melipona têm importante papel na polinização de plantas no Brasil, e por apresentar mecanismo peculiar de determinação de casta genético alimentar pode ser considerada um laboratório vivo para pesquisas de natureza molecular. O Hormônio Juvenil (HJ) é o principal hormônio que regula a diferenciação de casta e o polietismo etár
Publicado em: 2006
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4. Estudo da proteína de choque térmico GRP78 para o desenvolvimento de um sistema de receptor-ligante para o câncer de próstata / Use of the heat-shock protein GRP78 for the development of a receptor-ligand system in prostate cancer
Introduction: Despite the advances in diagnosis and treatment, advanced prostate cancer remains a lethal condition. Improved methods of therapy are needed to reduce the morbidity and mortality rates associated with this disease. The Glucose-regulated protein-78 (GRP78), a stress-responsive heat-shock protein involved in antigen presentation, was recently des
Publicado em: 2003
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5. Display of peptides and proteins on the surface of bacteriophage lambda.
The display of peptides or proteins on the surface of viruses is an important technology for studying peptides or proteins and their interaction with other molecules. Here we describe a display vehicle based on bacteriophage lambda that incorporates a number of features distinct from other currently used display systems. Fusions of peptides or protein domain
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6. Generation of Novel Single-Chain Antibodies by Phage-Display Technology to Direct Imaging Agents Highly Selective to Pancreatic β- or α-Cells In Vivo
American Diabetes Association.
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7. Molecular specialization of breast vasculature: A breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature
In vivo phage display identifies peptides that selectively home to the vasculature of individual organs, tissues, and tumors. Here we report the identification of a cyclic nonapeptide, CPGPEGAGC, which homes to normal breast tissue with a 100-fold selectivity over nontargeted phage. The homing of the phage is inhibited by its cognate synthetic peptide. Phage
The National Academy of Sciences.
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8. Molecular heterogeneity of the vascular endothelium revealed by in vivo phage display.
Vascular beds are known to differ in structure and metabolic function, but less is known about their molecular diversity. We have studied organ-specific molecular differences of the endothelium in various tissues by using in vivo screening of peptide libraries expressed on the surface of a bacteriophage. We report here that targeting of a large number of tis
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9. Generation of native bovine mAbs by phage display
Modeling of disease pathogenesis and immunity often is carried out in large animals that are natural targets for pathogens of human or economic relevance. Although murine mAbs are a valuable tool in identifying certain host/pathogen interactions, progress in comparative immunology would be enhanced by the use of mAbs isolated from the host species. Such anti
The National Academy of Sciences.
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10. Biotin-tagged cDNA expression libraries displayed on lambda phage: a new tool for the selection of natural protein ligands
cDNA expression libraries displayed on lambda phage have been successfully employed to identify partners involved in antibody–antigen, protein– protein and DNA–protein interactions and represent a novel approach to functional genomics. However, as in all other cDNA expression libraries based on fusion to a carrier polypeptide, a major issue of this sys
Oxford University Press.
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11. Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei
Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic
American Society for Microbiology.
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12. Phage Display of Adenovirus Type 5 Fiber Knob as a Tool for Specific Ligand Selection and Validation
Adenovirus (Ad) vectors are most potent for use as gene delivery vehicles to infect human cells in vitro and in vivo with high efficiency. The main limitation in utilization of Ad as a gene transfer vector is the lack of specificity. Genetic modifications of Ad capsid proteins resulting in incorporation of foreign polypeptide ligand sequences can redirect th
American Society for Microbiology.