Optical Distance Measurements
Mostrando 13-21 de 21 artigos, teses e dissertações.
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13. Laser triangulation rangefinders. / Medidores de distância por triangulação a laser.
Laser Triangulation Rangefinders are non-contact, optoelectronic devices for measurement of small displacements or distances. These devices offer a broad range of industrial applications. This work explains common configurations, parts and main components, comparing their advantages. A detailed project on a CCD based Laser Rangefinder is presented. Some indu
Publicado em: 1995
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14. Impact of Emission Anisotropy on Fluorescence Spectroscopy and FRET Distance Measurements
The objective of this report is to provide a practical and improved method for estimating Förster resonance energy transfer distance measurement error due to unknown angles in the dipole orientation factor based on emission anisotropy measurements. We improve on the method of Dale et al. (1979), which has minor mistakes and is frequently interpreted in over
The Biophysical Society.
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15. Depth measurement with the slit-lamp microscope.
The sagittal distance of ocular structures can be obtained with a slit-lamp microscope providing the movement of the slit-lamp can be measured. A simple device consisting of a pointer attached by a spring clip to the axle of a Haag-Streit 900 slit-lamp is described. The pointer rides over a scale which slides with the slit-lamp. Comparisons of anterior chamb
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16. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
We extend the sensitivity of fluorescence resonance energy transfer (FRET) to the single molecule level by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy (NSOM) is used to obtain simultaneous dual color images and emission spectra from donor and acceptor fluorophores link
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17. Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave
Fluorescence techniques for monitoring single-molecule dynamics in the vertical dimension currently do not exist. Here we use an atomic force microscope to calibrate the distance-dependent intensity decay of an evanescent wave. The measured evanescent wave transfer function was then used to convert the vertical motions of a fluorescent particle into displace
National Academy of Sciences.
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18. Nanometer-localized multiple single-molecule fluorescence microscopy
Fitting the image of a single molecule to the point spread function of an optical system greatly improves the precision with which single molecules can be located. Centroid localization with nanometer precision has been achieved when a sufficient number of photons are collected. However, if multiple single molecules reside within a diffraction-limited spot,
National Academy of Sciences.
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19. Comparison of time-resolved and -unresolved measurements of deoxyhemoglobin in brain.
Continuous (CW) and pulsed light were used for the noninvasive measurement of hemoglobin oxygenation in tissues. A dual wavelength method of continuous illumination spectroscopy used 760 nm (deoxyhemoglobin peak) and 800 nm (an oxyhemoglobin-deoxyhemoglobin isosbestic point) to measure the kinetics and extent of oxyhemoglobin deoxygenation in brains during m
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20. Formation and Ultrastructure of Extra Membranes in Escherichia coli
A temperature-sensitive strain of Escherichia coli (strain 0111a1) was shown to accumulate membranous structures at 40 C. These “extra membranes” appeared as vesicles or whorls (or both), depending on the time of growth at 40 C. After 2 hr of growth at 40 C, only vesicles were observed in E. coli 0111a1 cells; both vesicles and whorls were apparent after
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21. Decreased birefringence of the superficial zone collagen network in the canine knee (stifle) articular cartilage after long distance running training, detected by quantitative polarised light microscopy.
OBJECTIVE: To investigate the effects of a one year programme of running training (up to 40 km/day for 15 weeks) on the spatial orientation pattern of collagen and glycosaminoglycans in articular cartilage in different parts of the knee (stifle) and shoulder joints of young beagle dogs. METHODS: Area specific measurements of the optical path difference (= re