Open Column Chromatography
Mostrando 1-12 de 39 artigos, teses e dissertações.
-
1. A Gas Chromatography Method for Simultaneous Quantification of Inorganic Gases and Light Hydrocarbons Generated in Thermochemical Processes
This paper reports a method for simultaneous determination of H2, O2, N2, CO, CO2, CH4, C2H2, C2H4, C2H6, C3H4 (propadiene and propyne), C3H6, C3H8 and C4H10 (n-butane and isobutane) by gas chromatography using thermal conductivity and flame ionization detectors. A single porous layer open tubular column (0.53 mm internal diameter ? 30 m length ? 30 µm thic
J. Braz. Chem. Soc.. Publicado em: 2021-06
-
2. On-Flow Ligand Screening Assay Based on Immobilized Nucleoside Diphosphate Kinase B from Homo sapiens
We describe an on-flow zonal affinity-based chromatography assay to screen ligands for the nucleoside diphosphate kinase B enzyme (NME2) from Homo sapiens. For the first time, we have covalently immobilized NME2 on the surface of an open fused silica capillary reactor (NME2-ICER) and placed the reactor before the analytical column, which resulted in a two-di
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
-
3. Bioassay guided purification of cytotoxic natural products from a red alga Dichotomaria obtusata
ABSTRACT Different solvent extracts of Dichotomaria obtusata (J. Ellis & Solander) Lamark, Galaxauraceae, a red algae collected from the coast of Bushehr in the Persain Gulf, was investigated for its cytotoxic properties and chemical constituents. The fresh alga, after extraction with methanol and dichloromethane were combined and partitioned between water,
Rev. bras. farmacogn.. Publicado em: 2016-12
-
4. Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus
Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81UmL-1, specific activity 78 U mg-1 with purification fold of 130 and recovery 1.
Braz. J. Microbiol.. Publicado em: 11/04/2014
-
5. Avaliação dos processos de pré-tratamento da superfície da sílica fundida no preparo de colunas capilares inertes para cromatografia gasosa / Evaluation of the processes of pretreatment on the fused-silica surface for preparation of inert capillary columns for gas chromatography
The control of the inner surface in open tubular capillary columns has fundamental importance to reach a high performance in gas chromatography analysis. It was very studied during 1970´s and 1980´s, but has not interested the researches along last years, not excluding also, the possibility of commercial secrets regarding column preparation. The activity o
Publicado em: 2009
-
6. Isolamento e identificação de compostos com atividade antioxidante de uma nova variedade de própolis brasileira produzida por abelhas da espécie Apis mellifera / Isolation and identification of compounds with antioxidant activity in a novel variety of Brazilian propolis produced by bees from the species Apis mellifera
Propolis is known for its wide range of biological activities, such as antioxidant, antibacterial, anti-inflammatory, antiviral, among others. Its composition varies according to the region where it is collected and there is a great chemical diversity among the different types of propolis. The main constituents of propolis are the phenolic compounds, which a
Publicado em: 2007
-
7. Carotenóides e composição centesimal de ervilhas (Pisum sativum L.) cruas e processadas / Carotenoids and centesimal composition of raw and cooked peas (Pisum sativum L.)
A ervilha é um dos alimentos mais produzidos no mundo e, conseqüentemente, tem uma grande importância para a nutrição humana, sendo comercializada sob várias formas: fresca, seca, congelada e enlatada, necessitando de cozimento antes de ser consumida. Sendo assim, podem ocorrer alterações em sua composição durante esses processamentos. O presente t
Publicado em: 2007
-
8. Study of the metabolism of Xylaria sp., na endophyte fungus isolated from the fruits of Sapindus saponaria / Contribuição ao estudo do metabolismo micromolecular de Xylaria sp., um fungo endofítico isolado de Sapindus saponaria
This work reports the study of a surprising plant microorganism relationship, involving the plant Sapindus saponaria, which is rich in antigungal saponins, and an endophyte fungus isolated from the pericarpe of the fruits collected in the host plant, and identified as Xylaria sp. The main objective of this work was to understand the survival mechanism of thi
Publicado em: 2006
-
9. Análise de fármacos em material biológico: acoplamento microextração em fase sólida "no tubo" e cromatografia líquida de alta eficiência
A new solid phase microextraction (SPME) system, known as in-tube SPME, was recently developed using an open tubular fused-silica capilary column, instead of an SPME fiber, as the SPME device. On-line in-tube SPME is usually used in combination with high performance liquid chromatography. Drugs in biological samples are directly extracted and concentrated in
Química Nova. Publicado em: 2005-10
-
10. Expressão heteróloga, purificação e caracterização das proteínas humanas DCRA (Down Syndrome Critical Region Gene A) e DSCR8 (Down Syndrome Critical Region Gene 8).
Down syndrome is the most frequent cause of mental retardation affecting millions of people worldwide and results from full or partial trisomy of chromosome 21 (HC21). Rare cases of partial trisomy allowed the identification of a small region in HC21 common to all carriers called Down Syndrome Critical Region. The genes DCRA and DSCR8, mapped to this region,
Publicado em: 2004
-
11. Estudo de reações de 51Cr(VI) com acidos concentrados atraves de metodos cromatograficos
Com o intuito de se verificar a redução ácida de Cr(VI), na ausência de qualquer agente redutor convencional, foram estudados sistemas Cr(VI)-ácido utilizando Cr como marcador. A especiação dos produtos da reação do Cr(VI) com os ácidos concentrados (HCI, HF, HCIO4, CF3SO3H, HNO3 e H2SO4) foi realizada por cromatografia de troca iônica a baixa pre
Publicado em: 1998
-
12. Estudo de carotenoide e pro-vitamina A em alimentos
Many analytical methods have been developed with the purpose of establishing a simple, rapid and economic procedure for determining the provi tamins A in foods. In the present study, three methods were assessed: those of COST91, IVACG and RODRIGUEZ-AMAYA et alii simplied. Kale, carrot, papaya and tomato were chosen as food samples because of their distinct c
Publicado em: 1993