Nucleotide Sequence
Mostrando 13-24 de 12604 artigos, teses e dissertações.
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13. ETIQUETAS DE SECUENCIAS EXPRESADAS DIFERENCIALES DE FRUTOS DE AGUACATE RAZA MEXICANA (Persea americana Mill. var. drymifolia)
ABSTRACT In this study, Differential Expressed Sequence Tags (ESTs) of immature fruits of Persea americana Mill var. drymifolia of the state of Nuevo León, Mexico were developed and identified. Ten genotypes with fruits of different size and shape were selected to generate ESTs by the differential display technique. In total, 393 amplified differential fra
Rev. Bras. Frutic.. Publicado em: 15/02/2018
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14. Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain
BACKGROUND Leptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates. OBJECTIVES This study aimed to obtain the complete
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-02
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15. Molecular Cloning and Mrna Expression Analysis of Sichuan White Goose (Anser Cygnoides) Chrebp Gene
ABSTRACT The carbohydrate response element-binding protein (ChREBP) is an important nuclear factor that regulates glycolysis and de novo lipogenesis. However, the role of ChREBP in fatty liver development in geese remains unclear. In order to understand the function of ChREBP in lipid metabolism of geese, we first cloned the complete cDNA of the ChREBP of th
Rev. Bras. Cienc. Avic.. Publicado em: 2017-12
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16. Simple protocol for population (Sanger) sequencing for Zika virus genomic regions
BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE The aim of this study was to develop a simple and robu
Mem. Inst. Oswaldo Cruz. Publicado em: 27/11/2017
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17. The complete mitochondrial genome of Engyodontium album and comparative analyses with Ascomycota mitogenomes
Abstract Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-thro
Genet. Mol. Biol.. Publicado em: 23/10/2017
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18. Minimum free energy predicted base pairing in the 39 nt spliced leader and 5’ UTR of calmodulin mRNA from Trypanosoma cruzi: influence of the multiple trans-splicing sites
ABSTRACT We analyzed the compositional changes and the stable base pairs in the predicted secondary structure of the 5’ UTR calmodulin mRNA in T. cruzi. The three copies of calmodulin in T. cruzi genome display variable position of the trans splicing sites and give rise to several mRNA that differs slightly on 5’ UTR composition in the epimastigote stage
An. Acad. Bras. Ciênc.. Publicado em: 16/10/2017
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19. Molecular analysis of CIB4 gene and protein in Kermani sheep
The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Differe
Braz J Med Biol Res. Publicado em: 12/09/2017
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20. Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil
Abstract INTRODUCTION: Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collect
Rev. Soc. Bras. Med. Trop.. Publicado em: 2017-09
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21. A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis
Abstract Chloroplast development and chlorophyll (Chl) biosynthesis in plants are regulated by many genes, but the underlying molecular mechanisms remain largely elusive. We isolated a rice mutant named yss2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth. The mutant developed normal green leaves from
Genet. Mol. Biol.. Publicado em: 31/08/2017
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22. Genome-wide association study of pre-harvest sprouting resistance in Chinese wheat founder parents
Abstract Pre-harvest sprouting (PHS) is a major abiotic factor affecting grain weight and quality, and is caused by an early break in seed dormancy. Association mapping (AM) is used to detect correlations between phenotypes and genotypes based on linkage disequilibrium (LD) in wheat breeding programs. We evaluated seed dormancy in 80 Chinese wheat founder pa
Genet. Mol. Biol.. Publicado em: 10/07/2017
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23. Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil
BACKGROUND Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established. OBJECTIVES
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-07
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24. Calcium homeostasis modulator 1 ( CALHM1 ) polymorphisms in cattle
ABSTRACT: The calcium homeostasis modulator 1 gene (CALHM1), which is located on chromosome 10 in humans and on chromosome 26 in cattle, is a transmembrane glycoprotein that controls the cytosolic calcium concentrations. Altered calcium homeostasis has been associated with several neurodegenerative disorders, including Alzheimer’s disease (AD). In a recent
Pesq. Vet. Bras.. Publicado em: 2017-06