Nucleic Acids
Mostrando 1-12 de 1679 artigos, teses e dissertações.
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1. Gene Silencing Therapeutics in Cardiology: A Review Article
Abstract Therapeutics that inhibit enzymes, receptors, ion channels, and cotransporters have long been the mainstay of cardiovascular medicine. Now, oligonucleotide therapeutics offer a modern variation on this paradigm of protein inhibition. Rather than target a protein, however, small interfering ribonucleic acids and antisense oligonucleotides target the
International Journal of Cardiovascular Sciences. Publicado em: 2022
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2. Effects of Polycyclic Aromatic Hydrocarbon Pendant-Armed Ligands on the Catecholase Activity of Dinuclear Copper(II) Complexes
Herein, we present three new binuclear copper(II) complexes containing different polycyclic aromatic hydrocarbons, able to catalyze the oxidation of 3,5-di-tert-butylcathecol, a model substrate for catechol oxidase. The ligands and complexes were successful characterized in solid and solution states. The structure of C1 was determined by X-ray crystallograph
J. Braz. Chem. Soc.. Publicado em: 2021-05
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3. Specific Disulfide Bond Formation Directed by Peptide Nucleic Acids
In this study, we successfully demonstrated a strategy for specific disulfide bond formation by hybridization of two complementary peptide nucleic acid strands. Peptides were linked to the pair of complementary peptide nucleic acids by native chemical ligation, respectively, and base pairing drove the two peptides into close proximity for interchain disulfid
J. Braz. Chem. Soc.. Publicado em: 2020-05
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4. Human virome in nasopharynx and tracheal secretion samples
BACKGROUND In Brazil the implementation of the Sentinel Surveillance System of Influenza began in 2000. Central public health laboratories use reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for diagnosis of respiratory viruses, but this protocol identifies only specific targets, resulted in inconclusive diagnosis for many samples. T
Mem. Inst. Oswaldo Cruz. Publicado em: 03/10/2019
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5. Protocol adjustment improves the extraction of high-quality total RNA from common bean stems infected by Sclerotinia sclerotiorum
RESUMO O Straw Test é um ensaio desenvolvido para avaliar a resistência do feijoeiro ao mofo branco, no qual as hastes da planta são inoculados e os sintomas da doença são monitorados. É plausível admitir que a investigação da expressão gênica em tecidos infectados por patógenos possa ser estrategicamente interessante. No entanto, obter um RNA de
Ciênc. agrotec.. Publicado em: 03/06/2019
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6. CspB of an arctic bacterium, Polaribacter irgensii KOPRI 22228, confers extraordinary freeze-tolerance
ABSTRACT Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the co
Braz. J. Microbiol.. Publicado em: 2018-03
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7. Application of Next-generation Sequencing in Clinical Molecular Diagnostics
ABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy
Braz. arch. biol. technol.. Publicado em: 18/05/2017
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8. BIOMATERIAIS PARA FORMULAÇÕES DE BASE NANOTECNOLÓGICA VISANDO TERAPIA GENÉTICA OCULAR
Gene therapy is a promising tool for the treatment of ocular diseases. Nevertheless, there are some import limitations, especially related to the ocular route and the characteristics of nucleic acids that must be overcome to ensure the success of the therapy. In this context, nanotechnology-based carriers such as nanoemulsions, nanoparticles, dendrimers and
Quím. Nova. Publicado em: 2017-01
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9. In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validat
Braz. J. Microbiol.. Publicado em: 2016-12
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10. DEVELOPMENT OF A NEW PROCESS FOR PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM Streptococcus pneumoniae SEROTYPE 14
Abstract The main virulence factor of Streptococcus pneumoniae is the capsular polysaccharide (PS), which is the antigen of all current vaccines that are prepared with PS purified from serotypes prevalent in the population. In this work, three purification strategies were evaluated and a new process was developed for purification of serotype 14 PS (PS14), re
Braz. J. Chem. Eng.. Publicado em: 2016-09
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11. Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples
Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus
Mem. Inst. Oswaldo Cruz. Publicado em: 19/02/2016
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12. Intended and Unintended Consequences and Applications of Unnatural Interfaces: Oligo p-Phenylene Ethynylene Electrolytes, Biological Cells and Biomacromolecules
This short review focuses on an extended study of synthetic oligomericphenyleneethynylene (OPE) electrolytes that have been investigated for both their antimicrobial activity and their fluorescence sensing properties. In both cases, interfaces between these synthetic electrolytes and naturally occurring materials such as proteins, lipids, nucleic acids and c
J. Braz. Chem. Soc.. Publicado em: 2016-02