Mutagenesis
Mostrando 13-24 de 7701 artigos, teses e dissertações.
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13. Detection of differentially methylated regions of irradiated fig tree selections
ABSTRACT Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about th
Sci. agric. (Piracicaba, Braz.). Publicado em: 2017-08
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14. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production
Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was se
Braz. J. Microbiol.. Publicado em: 2017-07
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15. Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-06
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16. Structural determinants of the hyperalgesic activity of myotoxic Lys49-phospholipase A2
Abstract Background Bothropstoxin-I (BthTx-I) is a Lys49-phospholipase A2 (Lys49-PLA2) from the venom of Bothrops jararacussu, which despite of the lack of catalytic activity induces myotoxicity, inflammation and pain. The C-terminal region of the Lys49-PLA2s is important for these effects; however, the amino acid residues that determine hyperalgesia and e
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 16/03/2017
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17. Lipopeptide production by Bacillus subtilis R1 and its possible applications
Abstract The possible application of a bacterial strain - Bacillus subtilis R1, isolated from an oil contaminated desert site in India, as biocontrol agent and its biosurfactant in microbial enhanced oil recovery are discussed. The biosurfactant production in minimal medium was carried out at different temperatures and salt concentrations, where it produced
Braz. J. Microbiol.. Publicado em: 2016-12
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18. The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens
Abstract The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagen
Braz. J. Microbiol.. Publicado em: 2016-03
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19. Characterization of the
hrpZ gene fromPseudomonas syringae pv.maculicola M2Pseudomonas syringae pv.maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy inPseudomonas syringae pv.maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 mediuBraz. J. Microbiol.. Publicado em: 2015-09
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20. Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions
A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke collected from Shihezi (Xinjiang, China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an
Aspergillus niger strain by analysis of 16S rRNA. To improve inulinase production, this fungus was subjected to mutagenesiBraz. J. Microbiol.. Publicado em: 2015-09
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21. The Compact Irradiator Modulus Designed for DNA Repair and Mutagenesis Studies in ISS Microgravity Environment Using UVA Emitted by Light-Emitting Diodes
ABSTRACT: This work presents the design and characteristics of a new compact ultraviolet (UV) irradiator used in a biological onboard space flight experiment. The experiment, called DRM, took place in the International Space Station research facility (ISS-13 expedition), during the Centenary Mission (Russian-Brazil) in March-April 2006. The DRM main objectiv
J. Aerosp. Technol. Manag.. Publicado em: 2014-09
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22. Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo
The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess
Braz J Med Biol Res. Publicado em: 28/03/2014
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23. Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone nor
Braz J Med Biol Res. Publicado em: 2014-02
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24. DNA repair diseases: what do they tell us about cancer and aging?
The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) but later in many others, led to striking observations on the association of molecular defects and patients' clinical phenotypes. For example, patients with syndromes resulting from defective nucleotide excision repair (NER) or translesion synthesis (TLS) present
Genet. Mol. Biol.. Publicado em: 2014