Multipoint Immobilization
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase
We carried out the physico-chemical characterization of Immobead 150, a hydrophobic support for the immobilization of lipases. Thermomyces lanuginosus lipase (TLL) was immobilized on Immobead 150 by multipoint covalent attachment (ImmTLL) and the morphological, textural, structural, thermal, and physico-chemical properties of the support, before and after en
J. Braz. Chem. Soc.. Publicado em: 2017-08
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2. Produção de xilo-oligossacarídeos a partir de lignocelulósicos pré-tratados com xilanases imobilizadas e estabilzadas
Endoxylanase, an important industrial enzyme, cleaves ß-1,4-xylanosidic bounds in xylan chains, main plant hemicellulose component. Xylan can be explored to produce many products, among them xylo-oligosaccharides, XOS, made of xylose monomers (2 to 7 units). XOS are considered to be prebiotic ingredients and can improve humans health. XOS are produced by xy
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2012
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3. Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual
Biodiesel consiste de ésteres alquílicos de ácidos graxos produzidos pela transesterificação de triglicerídeos com álcoois de cadeia curta. Tradicionalmente, a reação ocorre na presença de catalisadores químicos, como álcalis ou ácidos. A utilização alternativa de lipases como biocatalisadores na reação de síntese do biodiesel não gera mat
Publicado em: 2009
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4. Imobilização e estabilização de D-Hidantoinase para a produção de N-Carbamoil-D-Fenilglicina
Immobilization and stabilization of enzymes increases their potential for use in industrial scale. D-hydantoinases (dihidropirimidina amidrohidrolase EC 3.5.2.2) catalyze the hydrolysis of D-hydantoins, generating the corresponding Ncarbamoil- D-amino acid and are used in the production of D-amino acids, including Dphenylglycine and D-p-hydroxyphenylglycine.
Publicado em: 2008
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5. Stabilization of penicillin G acylase by immobilization on glutaraldehyde-activated chitosan
The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold m
Brazilian Journal of Chemical Engineering. Publicado em: 2005-12
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6. Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment
Three mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested conditions (e.g., pH 2.5 at 4°C, pH 5 at 60°C, pH 7 at 55°C, or 6
American Society for Microbiology.