Middlebrook
Mostrando 1-12 de 110 artigos, teses e dissertações.
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1. Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity
ABSTRACT Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing grow
Braz. J. Pharm. Sci.. Publicado em: 14/05/2018
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2. Comparative study of Mycobacterium bovis primary isolation methods
Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian sampl
Braz. J. Microbiol.. Publicado em: 2017-03
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3. Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The id
Braz. J. Microbiol.. Publicado em: 02/05/2014
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4. Comparação entre meios de cultura e condições de incubação para o primo isolamento de Mycobacterium bovis de bovinos brasileiros / Comparison between media and incubation conditions for primary isolation of Mycobacterium bovis from Brazilian cattle
Considerando que os meios de cultura e as condições de incubação são os principais fatores para o sucesso do primo isolamento, além do método de descontaminação, quatro meios de cultura e três condições de incubação foram investigados. Noventa e sete amostras de lesões granulomatosas foram submetidas ao método de descontaminação com cloreto
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/06/2011
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5. Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria
A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jense
Brazilian Journal of Microbiology. Publicado em: 2011-09
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6. Associação da técnica de cultivo em camada delgada contendo ágar Middlebrook 7H11 Modificado com a Reação em Cadeia de Polimerase (PCR) para identificação precoce de Mycobacterium bovis em órgãos de bovinos e bubalinos oriundos de abatedouros comerciais / Association between the modified Middlebrook 7H11 agar thin layer cultivation technique with the Polymerase Chain Reaction (PCR) on the earlier identification of Mycobacterium bovis in bovine and buffalo organs deriving from commercial slaughterhouses
The modified thin layer Middlebrook 7H11 cultivation was compared to the standard culture technique containing Stonebrink media, in order to evaluate the sensitivity and time for the detection of Mycobacterium bovis in bovine and buffalo organs deriving from commercial slaughterhouses. The PCR was applied for confirmation of the growth observed in the cultur
Publicado em: 2010
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7. Comparação de duas técnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita clínica de paratuberculose
A paratuberculose é uma enterite crônica granulomatosa causada por Mycobacterium avium subsp. paratuberculosis que afeta principalmente os ruminantes. A cultura de bactérias a partir de amostras de fezes e tecidos constitui um dos métodos mais eficazes de diagnóstico, sendo ainda o único método disponível para obtenção de isolamentos e estirpes de
Pesquisa Veterinária Brasileira. Publicado em: 2009-05
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8. Avaliação da atividade micobactericida de desinfetantes químicos utilizando a técnica de cultivo em camada de ágar Middlebrook 7H11 / Evaluation of mycobactericidal activity of chemical disinfectants using the Middlebrook 7H11 agar medium technique
The mycobactericidal activity of five chemical disinfectants was evaluated against a strain of Mycobacterium bovis isolated from a goat, typified by PCR (polymerase chain reaction) and with 32 days of growth in the Stonebrink medium. The disinfectants were tested using the modified thin layer Middlebrook 7H11 cultivation technique and it was compared to the
Publicado em: 2007
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9. Utilização de uma técnica rápida para o isolamento de Mycobacterium bovis a partir de amostras de leite experimentalmente inoculadas / Utilization of the fast technique of isolation of Mycobacterium bovis from milk samples experimentally inoculated
A técnica de camada delgada em placas com meio de Middlebrook 7H11 foi comparada com a técnica padrão de cultura em meio de Stonebrink, a fim de se avaliar a sensibilidade e o tempo de detecção de micobactérias em amostras de leite, experimentalmente inoculadas com Mycobacterium bovis (estirpe AN5), em uma diluição 10-2, e submetidas a duas diferente
Publicado em: 2005
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10. Comparison of a radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear-negative specimens.
The BACTEC system and three conventional media (Middlebrook 7H10, selective Middlebrook 7H11 [S7H11], and Lowenstein-Jensen [LJ] were compared for their mean recovery times and recovery rates of mycobacteria from acid-fast, smear-negative clinical specimens. Of the 71 smear-negative, culture-positive specimens recovered from 2,165 submitted smear-negative cu
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11. Comparison of the Mycobacteria Growth Indicator Tube with MB Redox, Löwenstein-Jensen, and Middlebrook 7H11 Media for Recovery of Mycobacteria in Clinical Specimens
The rate of recovery and the mean time to detection of mycobacteria in clinical specimens were evaluated with two nonradiometric broth-based systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox systems. The data obtained for each system were compared with each other and with those obtained with the Löwenstein-Jensen (LJ) and Middlebrook 7H11
American Society for Microbiology.
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12. Evaluation of the ESP Culture System II for recovery of mycobacteria from blood specimens collected in isolator tubes.
The reliability of the ESP Culture System II (ESP II; AccuMed International, Westlake, Ohio), a continuously monitoring, nonradiometric mycobacterial culture system, for recovery of mycobacteria from sediments of blood collected in an Isolator tube was evaluated by comparing its performance to inoculation of the sediment onto Middlebrook 7H11/7H11 selective