Metagenomic Libraries
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Triagem, aplicação e engenharia de biocatalisadores para transformações enantio e regiosseletivas / Screening, applying and engineering biocatalysts for enantio and regioselective transformations
Biocatalysts have been widely applied in recent decades for industrial processes yielding high value products under environmentally friendly reaction conditions. The development of biocatalysts often begins with screening to identify enzymes with suitable activities followed by characterization of the enzymes chemo-, regio- and stereoselectivity or stability
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/06/2011
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2. Bioprospecção de genes envolvidos na síntese de biossurfactantes a apartir de microbiota de resevatórios de petróleo / Bioprospection of genes involved in the synthesis of biosurfactants from braziliam petroleum reservoirs microbial communities
Bioemulsifiers or biosurfactants are compounds produced by microorganisms which enhance the bioavailability of hydrophobic substances promoting the stabilization of substances with different polarities, like oil-in-water emulsion occurring in petroleum reservoirs. The samples used in this work were collected in the Potiguar Basin (RN, Brazil), and employed a
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/07/2010
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3. Prospecção de genes de interesse biotecnológico : uma abordagem metagenômica
The total number of prokaryotic cells on Earth has been estimated at 4 to 6x1030 and only about 1% of microorganisms present in the environment can be cultivated by standard techniques of cultivation and plating. Therefore, it is a huge biological and genetic pool that can be exploited, for the identification and characterization of genes with biotechnologic
Publicado em: 2009
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4. Polyketide biosynthetic gene bioprospection in metagenomic DNA from Atlantic Forest soil. / Bioprospecção de genes biossintéticos de policetídeos em DNA metagenômico de solo de Mata Atlântica.
The Brazilian Atlantic Forest is considered as one of the most important reservoir of biodiversity in the planet. This biome is extremely important for its richness of plant and animal species but although with their composition and interactions poorly known and unexplored from a microbiological perspective. One gram of soil can contain near 10 billion micro
Publicado em: 2009
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5. Triagem de enzimas associadas à biotransformação de hidrocarbonetos a partir de metagenoma de sedimentos contaminados com petroléo e metais pesados / Screening of Enzymes Related to Biotransformation of Hydrocarbons from Metagenome of Contaminated Sediments with Oil and Heavy Metals
A metagenômica trouxe novas perspectivas ao estudo de comunidades microbianas no ambiente, permitindo explorar tanto a diversidade taxonômica de microrganismos ainda não-cultivados, como o acesso direto a genes e vias metabólicas. Neste trabalho, foram construídas bibliotecas metagenômicas a partir de amostras de sedimentos de mangue da Baía de Guanab
Publicado em: 2009
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6. Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms
Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. To develop methods to investigate the full extent of microbial diversity, we used a bacterial art
American Society for Microbiology.
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7. Metagenomic Profiling: Microarray Analysis of an Environmental Genomic Library
Genomic libraries derived from environmental DNA (metagenomic libraries) are useful for characterizing uncultured microorganisms. However, conventional library-screening techniques permit characterization of relatively few environmental clones. Here we describe a novel approach for characterization of a metagenomic library by hybridizing the library with DNA
American Society for Microbiology.
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8. Phylogenetic Analysis of Polyketide Synthase I Domains from Soil Metagenomic Libraries Allows Selection of Promising Clones
The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industrial interest. Among the PKSI domains, the
American Society for Microbiology.
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9. Construction and Screening of Metagenomic Libraries Derived from Enrichment Cultures: Generation of a Gene Bank for Genes Conferring Alcohol Oxidoreductase Activity on Escherichia coli
Enrichment of microorganisms with special traits and the construction of metagenomic libraries by direct cloning of environmental DNA have great potential for identifying genes and gene products for biotechnological purposes. We have combined these techniques to isolate novel genes conferring oxidation of short-chain (C2 to C4) polyols or reduction of the co
American Society for Microbiology.
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10. Identification and Characterization of Coenzyme B12-Dependent Glycerol Dehydratase- and Diol Dehydratase-Encoding Genes from Metagenomic DNA Libraries Derived from Enrichment Cultures
To isolate genes encoding coenzyme B12-dependent glycerol and diol dehydratases, metagenomic libraries from three different environmental samples were constructed after allowing growth of the dehydratase-containing microorganisms present for 48 h with glycerol under anaerobic conditions. The libraries were searched for the targeted genes by an activity scree
American Society for Microbiology.
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11. Genomic Signature Tags (GSTs): A System for Profiling Genomic DNA
Genomic signature tags (GSTs) are the products of a method we have developed for identifying and quantitatively analyzing genomic DNAs. The DNA is initially fragmented with a type II restriction enzyme. An oligonucleotide adaptor containing a recognition site for MmeI, a type IIS restriction enzyme, is then used to release 21-bp tags from fixed positions in
Cold Spring Harbor Laboratory Press.
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12. New Thermophilic and Thermostable Esterase with Sequence Similarity to the Hormone-Sensitive Lipase Family, Cloned from a Metagenomic Library
A gene coding for a thermostable esterase was isolated by functional screening of Escherichia coli cells that had been transformed with fosmid environmental DNA libraries constructed with metagenomes from thermal environmental samples. The gene conferring esterase activity on E. coli grown on tributyrin agar was composed of 936 bp, corresponding to 311 amino
American Society for Microbiology.