Metagenome
Mostrando 13-24 de 25 artigos, teses e dissertações.
-
13. ExploraÃÃo biotecnolÃgica de microrganismos de solo de cerrado atravÃs da construÃÃo de bibliotecas metagenÃmicas e tÃcnicas de cultivo
The Cerrado is Brazilâs second biggest biome (22%), distinguished for its fauna and flora diversity. Few studies about its soil microbiane diversity have been carried out, and the main reason that the knowledge about microorganisms is limited is that, until not long ago, these had to be cultivated in order to be described. Of a cerrado soil sample, metageno
Publicado em: 2006
-
14. Crystallization and preliminary X-ray diffraction analysis of a putative two-domain-type laccase from a metagenome
A putative two-domain-type laccase from a metagenome was crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to a resolution of 1.7 Å.
International Union of Crystallography.
-
15. Novel Tetracycline Resistance Determinant from the Oral Metagenome
A major drawback of most studies on how bacteria become resistant to antibiotics is that they concentrate mainly on bacteria that can be cultivated in the laboratory. In the present study, we cloned part of the oral metagenome and isolated a novel tetracycline resistance gene, tet(37), which inactivates tetracycline.
American Society for Microbiology.
-
16. Prospecting for Novel Biocatalysts in a Soil Metagenome
The metagenomes of complex microbial communities are rich sources of novel biocatalysts. We exploited the metagenome of a mixed microbial population for isolation of more than 15 different genes encoding novel biocatalysts by using a combined cultivation and direct cloning strategy. A 16S rRNA sequence analysis revealed the presence of hitherto uncultured mi
American Society for Microbiology.
-
17. Metagenome Survey of Biofilms in Drinking-Water Networks
Most naturally occurring biofilms contain a vast majority of microorganisms which have not yet been cultured, and therefore we have little information on the genetic information content of these communities. Therefore, we initiated work to characterize the complex metagenome of model drinking water biofilms grown on rubber-coated valves by employing three di
American Society for Microbiology.
-
18. Phylogenetic Diversity and Metabolic Potential Revealed in a Glacier Ice Metagenome▿ †
The largest part of the Earth's microbial biomass is stored in cold environments, which represent almost untapped reservoirs of novel species, processes, and genes. In this study, the first metagenomic survey of the metabolic potential and phylogenetic diversity of a microbial assemblage present in glacial ice is presented. DNA was isolated from glacial ice
American Society for Microbiology (ASM).
-
19. HAPLOWSER: a whole-genome haplotype browser for personal genome and metagenome
Summary: Haplotype assembly is becoming a very important tool in genome sequencing of human and other organisms. Although haplotypes were previously inferred from genome assemblies, there has never been a comparative haplotype browser that depicts a global picture of whole-genome alignments among haplotypes of different organisms. We introduce a whole-genome
Oxford University Press.
-
20. The bag or the spindle: the cell factory at the time of systems' biology
Genome programs changed our view of bacteria as cell factories, by making them amenable to systematic rational improvement. As a first step, isolated genes (including those of the metagenome), or small gene clusters are improved and expressed in a variety of hosts. New techniques derived from functional genomics (transcriptome, proteome and metabolome studie
BioMed Central.
-
21. Chitinase Gene Sequences Retrieved from Diverse Aquatic Habitats Reveal Environment-Specific Distributions
Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a ∼900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presumptive chitinase genes were created for nine water and
American Society for Microbiology.
-
22. Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei
Bacterial symbionts have long been suspected to be the true producers of many drug candidates isolated from marine invertebrates. Sponges, the most important marine source of biologically active natural products, have been frequently hypothesized to contain compounds of bacterial origin. This symbiont hypothesis, however, remained unproven because of a gener
National Academy of Sciences.
-
23. Characterization of a Novel Amylolytic Enzyme Encoded by a Gene from a Soil-Derived Metagenomic Library
It has been estimated that less than 1% of the microorganisms in nature can be cultivated by conventional techniques. Thus, the classical approach of isolating enzymes from pure cultures allows the analysis of only a subset of the total naturally occurring microbiota in environmental samples enriched in microorganisms. To isolate useful microbial enzymes fro
American Society for Microbiology.
-
24. Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms
Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. To develop methods to investigate the full extent of microbial diversity, we used a bacterial art
American Society for Microbiology.