Large Cap
Mostrando 13-24 de 157 artigos, teses e dissertações.
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13. Sintese de nanoparticulas de fosfatos de calcio em ambientes confinados no sistema Renex-100 / cicloexano / "brine"
Bone is formed by nanometric calcium phosphate particles, organized through a collagen matrix. Synthetic calcium phosphates with low crystallinity and high surface area (SA) may lead to the understanding of bone formation process and to the improvement of implants, through the mimesis of the natural bone behaviour. Nanometric calcium phosphate particles were
Publicado em: 2002
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14. Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.
The nucleotide sequence of the mitochondrial DNA (mtDNA) in the region coding for the 3' end of the large rRNA has been determined for two human cell lines bearing independent cytoplasmic chloramphenicol-resistant (CAP-r) mutations. Comparison of the sequences of these two phenotypically different CAP-r mutants with their CAP-sensitive (CAP-s) parental cell
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15. Large-scale induced fit recognition of an m7GpppG cap analogue by the human nuclear cap-binding complex
The heterodimeric nuclear cap-binding complex (CBC) binds to the 5′ cap structure of RNAs in the nucleus and plays a central role in their diverse maturation steps. We describe the crystal structure at 2.1 Å resolution of human CBC bound to an m7GpppG cap analogue. Comparison with the structure of uncomplexed CBC shows that cap binding induces co-operat
Oxford University Press.
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16. Genetic and biochemical analysis of the adenylyl cyclase-associated protein, cap, in Schizosaccharomyces pombe.
We have identified, cloned, and studied a gene, cap, encoding a protein that is associated with adenylyl cyclase in the fission yeast Schizosaccharomyces pombe. This protein shares significant sequence homology with the adenylyl cyclase-associated CAP protein in the yeast Saccharomyces cerevisiae. CAP is a bifunctional protein; the N-terminal domain appears
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17. Control of Uridine Diphosphate-Glucose Dehydrogenase Synthesis and Uridine Diphosphate-Glucuronic Acid Accumulation by a Regulator Gene Mutation in Escherichia coli K-12
Uridine diphosphate (UDP)-glucose dehydrogenase, the enzyme that converts UDP-glucose to UDP-glucuronic acid, was derepressed in a mucoid (capR9) strain of Escherichia coli K-12 and repressed in a nonmucoid (capR+) strain. A nonmucoid mutant (strain MC 152; capR9 non-2) derived from the mucoid strain accumulated large quantities of nucleotides. Among these n
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18. New Antibiotic Resistance Loci in the Ribosomal Region of Yeast Mitochondrial DNA
A large number of mitochondrial antibiotic-resistant mutants have been isolated following mutagenesis with manganese. These include several different phenotypic classes of mutants, as distinguished by cross-resistance patterns, that have been found to be allelic at cap1 or ery1; some have been found to be heteroallelic.——Seven chloramphenicol-resistant m
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19. The Pre-mRNA 5′ Cap Determines Whether U6 Small Nuclear RNA Succeeds U1 Small Nuclear Ribonucleoprotein Particle at 5′ Splice Sites
Efficient splicing of the 5′-most intron of pre-mRNA requires a 5′ m7G(5′)ppp(5′)N cap, which has been implicated in U1 snRNP binding to 5′ splice sites. We demonstrate that the cap alters the kinetic profile of U1 snRNP binding, but its major effect is on U6 snRNA binding. With two alternative wild-type splice sites in an adenovirus pre-mRNA, the
American Society for Microbiology.
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20. Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
A 20.5-kb contiguous DNA fragment from Staphylococcus aureus Becker affecting type 8 capsule (CP8) biosynthesis was previously cloned. Sequencing analysis indicated that 16 open reading frames (ORFs) encoded within this fragment might be involved in CP8 synthesis. Using various plasmids containing DNA inserts derived from the 20.5-kb region, we showed by com
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21. Ribose 2′-O Methylation of the Vesicular Stomatitis Virus mRNA Cap Precedes and Facilitates Subsequent Guanine-N-7 Methylation by the Large Polymerase Protein▿
During conventional mRNA cap formation, two separate methyltransferases sequentially modify the cap structure, first at the guanine-N-7 (G-N-7) position and subsequently at the ribose 2′-O position. For vesicular stomatitis virus (VSV), a prototype of the nonsegmented negative-strand RNA viruses, the two methylase activities share a binding site for the me
American Society for Microbiology (ASM).
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22. Derepression of Uridine Diphosphate-Glucose Pyrophosphorylase (galU) in capR(lon), capS, and capT Mutants and Studies on the galU Repressor
Mutation of the capR(lon), capS, or capT genes in Escherichia coli K-12 causes overproduction of capsular polysaccharide leading to a mucoid phenotype. Several of the enzymes involved in capsular polysaccharide synthesis are derepressed in cap mutants. Previously it was shown that uridine diphosphate-glucose (UDPG) pyrophosphorylase, an enzyme involved in th
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23. Antigen Cap Formation in Cultured Fibroblasts: A Reflection of Membrane Fluidity and of Cell Motility
Reaction of antibody to surface Histocompatibility-2 antigens of cultured mouse fibroblasts causes aggregation of the cellular antigens into caps; these appear as areas of high antigen concentration localized away from the cell processes. Cap formation is inhibited when ATP generation by cells is interrupted, when the temperature is lowered, by addition of c
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24. Correlation of Pectin Methylesterase Activity in Root Caps of Pea with Root Border Cell Separation.
We tested predictions of the hypothesis that pectin methylesterase in the root cap plays a role in cell wall solubilization leading to separation of root border cells from the root tip. Root cap pectin methylesterase activity was detected only in species that release large numbers of border cells daily. In pea (Pisum sativum) root caps, enzyme activity is co