Isothermal Titration Calorimetry
Mostrando 1-12 de 90 artigos, teses e dissertações.
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1. Multifunctionality of βCD/Ofloxacin and HPβCD/Ofloxacin Complexes: Improvement of the Antimicrobial Activity and Apoptosis Induction on Lung Adenocarcinoma A549 Cells
The ofloxacin (OFLOX) is a second-generation synthetic antibiotic that can be classified as a multifunctional drug, but is a poorly soluble drug, which influences its efficiency. The inclusion complexes of OFLOX with β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD) can improve the chemical characteristics of the drug; however, studies showing
J. Braz. Chem. Soc.. Publicado em: 2020-12
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2. Effect of Acetonitrile and N,N-Dimethylformamide on the Formation of Poly(ethylene oxide)-Sodium Alkyl Sulfate Aggregates
The effects of low contents of acetonitrile (AN) or N,N-dimethylformamide (DMF) on the aggregation of sodium alkyl sulfate surfactants, in the absence and presence of poly(ethylene oxide) (PEO), were investigated using conductivity and isothermal titration calorimetry. The cosolvents slightly changed the critical micellar concentrations and did not alter the
J. Braz. Chem. Soc.. Publicado em: 2018-01
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3. Characteristics and thermodynamics of the interaction of 6-shogaol with human serum albumin as studied by isothermal titration calorimetry
ABSTRACT The interaction between 6-shogaol, a pharmacologically active ginger constituent, and human serum albumin (HSA), the main in vivo drug transporter, was investigated using isothermal titration calorimetry (ITC). The value of the binding constant, Ka (5.02 ± 1.37 × 104 M−1) obtained for the 6-shogaol-HSA system suggested intermediate affinity. Ana
Braz. J. Pharm. Sci.. Publicado em: 2016-09
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4. Recombinant Erwinia carotovora l-asparaginase II production in Escherichia coli fed-batch cultures
Asparaginases are the cornerstone therapy of many successful combination regimens for the treatment of acute lymphoblastic leukemia (ALL), the most common malignancy in children and adolescents. The aim of this work was to produce recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures. Using a robust fed-batch technique with
Braz. J. Chem. Eng.. Publicado em: 2013-06
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5. Investigação eletroquímica e calorimétrica da interação de novos agentes antitumorais biscatiônicos com DNA
Biscationic amidines bind in the DNA minor groove and present biological activity against a range of infectious diseases. Two new biscationic compounds (bis-α,ω-S-thioureido, amino and sulfide analogues) were synthesized in good yields and fully characterized, and their interaction with DNA was also investigated. Isothermal titration calorimetry (ITC) was
Química Nova. Publicado em: 2012
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6. Caracterização e interação do domínio C-terminal da chaperona Hsp90 humana e das co-chaperonas Tom 70 e Hop / Characterization and interaction of the C-terminal domain of the human chaperone Hsp90 and co-chaperones Tom 70 and Hop
A função biológica das proteínas está relacionada à sua estrutura tridimensional adquirida pelo processo de enovelamento protéico. Neste contexto, proteínas denominadas, genericamente, de chaperonas moleculares exercem papel fundamental atuando no auxílio do enovelamento correto, no reenovelamento e na dissociação de agregados protéicos. A Hsp90
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/08/2011
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7. Planejamento de inibidores da enzima diidroorotato desidrogenase de Trypanosoma cruzi por biocalorimetria / Biocalorimetry as a tool for Trypanosoma cruzi dihydroorotate dehydrogenase inhibitors discovery
A doença de Chagas, causada pelo protozoário flagelado Trypanosoma cruzi, é uma doença tropical que enseja morte/morbidade de milhões de pessoas na América Latina. Por processos migratórios, vem-se estendendo ao sul dos Estados Unidos, Canadá, Europa, Austrália e Japão. Essa doença tem sido considerada super-negligenciada pela indústria farmacêu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/03/2011
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8. O estudo da enzima deidroquinato sintase de Mycobacterium tuberculosis H37Rv como alvo para o desenvolvimento de fármacos antituberculose
Apesar da incidência per capita da tuberculose (TB) ter se mantido estável em 2005, o número de novos casos que surgem a cada ano continua a aumentar no mundo todo. De acordo com a Organização Mundial de Saúde, foram estimados 9,4 milhões de novos casos de TB em 2008, dos quais 1,4 milhões eram HIV - positivos, e com 1,8 milhões de mortes - o equiva
Publicado em: 2010
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9. Estudo fitoquímico de Euxylophora paraensis e avaliação das substâncias isoladas frente à enzima gliceraldeído-3-fosfato desidrogenase de Trypanosoma cruzi / PHYTOCHEMICAL STUDY OF Euxylophora paraensis AND EVALUATION OF SUBSTANCES ISOLATED FRONT OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OF Trypanosoma cruzi
This work describe the phytochemical study of bark and leaves of the specie E. paraensis (Rutaceae). This dissertation aims at contributing with the chemistry of the Rutaceae family and evaluation of the antichagasic activity of substances isolated. The phythochemical study allowed to isolate fifteen secondary metabolites belonging to different classes: two
Publicado em: 2008
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10. Nanoagregados baseados em ciclodextrinas em associação com a tetraciclina: caracterização fisico-química e avaliação antimicrobiana
Molecular self-assembling has been shown to be an efficient method to produce structures of few hundreds of nanometers in size. The supramolecular compounds made from (R)- cyclodextrin:tetracycline in aqueous solution were evaluated. The physicochemical interactions between â-cyclodextrin:tetracycline were characterized by dynamic light scattering (DLS), is
Publicado em: 2007
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11. Planejamento de inibidores da enzima gliceraldeído-3-fosfato desidrogenase de Trypanosoma cruzi por biocalorimetria / Structure-based inhibitor design of the Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase enzyme using isothermal titration calorimetry
A identificação de compostos bioativos que atuem em proteínas é imprescindível no conceito atual da Química Medicinal. Quase todos os processos biossintéticos dos organismos vivos são regulados por enzimas. Dentro deste contexto a enzima glicossomal Gliceraldeído-3-Fosfato desidrogenase (GAPDH) do Tripanosoma cruzi (agente etiológico causador da Do
Publicado em: 2007
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12. Estratégias para prospecção e predição de peptídeos bioativos
Chapters one and two deal with the isolation and identification of bioactive peptides from the skin secretion of Phyllomedusa hypochondrialis. The first chapter demonstrates the use of protemic techniques, mainly mass spectrometry, for the study of bradykinin related peptides (BRPs) present in the amphibians secretion and freshly dissected skin fragments. Ei
Publicado em: 2007