Inhalational Anesthetic
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Estudo dos efeitos do sevoflurano, propofol e sufentanil sobre o miocárdio na lesão de isquemia e reperfusão: estudo experimental em ratos / Study on the effects of sevoflurane, propofol and sufentanil on the myocardial ischemia and reperfusion injury: an experimental study in rats
A interrupção do fluxo sanguíneo, ou isquemia, representa um dos problemas mais importantes de doenças cardiovasculares e cerebrovasculares enfrentados pelos médicos na sua rotina. Em relação ao miocárdio muitos estudos têm sido realizados nessa área e sabe-se que os anestésicos inalatórios e os opiódes podem protege as células cardíacas contr
Publicado em: 2010
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2. An inhalational anesthetic binding domain in the nicotinic acetylcholine receptor.
To determine inhalational anesthetic binding domains on a ligand-gated ion channel, I used halothane direct photoaffinity labeling of the nicotinic acetylcholine receptor (nAChR) in native Torpedo membranes. [14C]Halothane photoaffinity labeling of both the native Torpedo membranes and the isolated nAChR was saturable, with Kd values within the clinically re
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3. A Unitary Anesthetic Binding Site at High Resolution*
Propofol is the most widely used injectable general anesthetic. Its targets include ligand-gated ion channels such as the GABAA receptor, but such receptor-channel complexes remain challenging to study at atomic resolution. Until structural biology methods advance to the point of being able to deal with systems such as the GABAA receptor, it will be necessar
American Society for Biochemistry and Molecular Biology.
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4. A Genetic and Mosaic Analysis of a Locus Involved in the Anesthesia Response of Drosophila Melanogaster
We describe a genetic and behavioral analysis of several alleles of har38, a mutant with altered sensitivity to the general anesthetic halothane. We obtained a P-element-induced allele of har38 and generated several excision alleles by remobilizing the P element. The mutants narrow abdomen (na) and har85 are confirmed to be allelic to har38. Besides a decrea
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5. Perioperative use of selective alpha-2 agonists and antagonists in small animals
Alpha-2 agonists are the only single class of anesthetic drugs that induce reliable, dose-dependent sedation, analgesia, and muscle relaxation in dogs and cats. Used at low doses, as adjuncts to injectable and inhalational anesthetics, selective alpha-2 agonists dramatically reduce the amount of anesthetic drug required to induce and maintain anesthesia. Thi
Canadian Veterinary Medical Association.
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6. Volatile anesthetics block actin-based motility in dendritic spines
Dendritic spines form the postsynaptic contact sites for most excitatory synapses in the brain. Spines occur in a wide range of different shapes that can vary depending on an animal’s experience or behavioral status. Recently we showed that spines on living neurons can change shape within seconds in a process that depends on actin polymerization. We have n
The National Academy of Sciences.
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7. Effect of halothane on the replication of animal viruses.
Five RNA- and two DNA-containing viruses were propagated in Vero cells and tested for their ability to replicate in the presence of halothane (2-bromo-2-chloro-1,1,1-trifluoroethane), a commonly used inhalational anesthetic. Halothane did not affect poliovirus replication at any anesthetic concentration tested, but all other viruses were either partially or
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8. Subcellular distribution of an inhalational anesthetic in situ.
To better understand the mechanisms and sites of anesthetic action, we determined the subcellular partitioning of halothane in a tissue model. A method was found to fix the in vivo distribution of halothane in rat atrial tissue for subsequent electron microscopy and x-ray microanalysis. Atrial strips were exposed to various concentrations of halothane, rapid
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9. Mechanism of Interaction between the General Anesthetic Halothane and a Model Ion Channel Protein, III: Molecular Dynamics Simulation Incorporating a Cyanophenylalanine Spectroscopic Probe
A nitrile-derived amino acid, PheCN, has been used as an internal spectroscopic probe to study the binding of an inhalational anesthetic to a model membrane protein. The infrared spectra from experiment showed a blue-shift of the nitrile vibrational frequency in the presence of the anesthetic halothane. To interpret the infrared results and explore the natur
The Biophysical Society.
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10. Isoflurane Inhibits the Neurotransmitter Release Machinery
Despite their importance, the mechanism of action of general anesthetics is still poorly understood. Facilitation of inhibitory GABAA receptors plays an important role in anesthesia, but other targets have also been linked to anesthetic actions. Anesthetics are known to suppress excitatory synaptic transmission, but it has been difficult to determine whether
American Physiological Society.
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11. Volatile anesthetic facilitation of in vitro desensitization of membrane-bound acetylcholine receptor from Torpedo californica
Incubation of membrane fragments bearing acetylcholine receptors from Torpedo californica under an atmosphere of 3% halothane, 1% chloroform, or 6% diethyl ether greatly facilitates the carbamoylcholine-induced structural transition of the acetylcholine receptor reflected by alterations in the rate of binding of 125I-labeled α-bungarotoxin. The half-time of
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12. Mechanism of Interaction between the General Anesthetic Halothane and a Model Ion Channel Protein, II: Fluorescence and Vibrational Spectroscopy Using a Cyanophenylalanine Probe
We demonstrate that cyano-phenylalanine (PheCN) can be utilized to probe the binding of the inhalational anesthetic halothane to an anesthetic-binding, model ion channel protein hbAP-PheCN. The Trp to PheCN mutation alters neither the α-helical conformation nor the 4-helix bundle structure. The halothane binding properties of this PheCN mutant hbAP-PheCN, b
The Biophysical Society.