In Vitro Mutagenesis
Mostrando 13-24 de 1444 artigos, teses e dissertações.
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13. Production and characterization of Mycoplasma hyopneumoniae recombinant proteins with potential for use in immunodiagnosis and vaccination / Produção e caracterização de proteínas recombinantes de Mycoplasma hyopneumoniae com potencial para uso em imunodiagnóstico e vacinação
Mycoplasma hyopneumoniae é o agente etiológico da pneumonia enzoótica suína (PES), uma doença respiratória responsável por significativas perdas econômicas. Vacinas comerciais são mundialmente utilizadas no controle desta doença, porém proporcionam apenas proteção parcial. Além disso, são caras devido ao crescimento fastidioso do M. hyopneumon
Publicado em: 2008
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14. InduÃÃo de mutaÃÃo e seleÃÃo em feijÃo-caupi [Vigna unguiculata (L.) Walp.] visando tolerÃncia à salinidade
O feijÃo-caupi [Vigna unguiculata (L.) Walp.] Ã uma importante cultura para a economia e nutriÃÃo dos povos de paÃses em desenvolvimento, em especial para a populaÃÃo do Nordeste brasileiro. Apesar da sua capacidade de cultivo nas mais diversas condiÃÃes de solo e clima (rusticidade), Ã uma cultura que ainda sofre com grandes perdas de produÃÃo d
Publicado em: 2007
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15. Evaluation of the mutagenic potential and in vitro metabolization of three anti-inflammatory parsalmide analogues / Avaliação do potencial mutagenico e metabolização in vitro de tres analogos do antiinflamatorio parsalmide
In this study, the mutagenicity of the anti-inflammatory parsaJmide [5-amino-N-butyl-2-(2-propynyloxy)-benzamide] analogues PA7 [5-anuno-N-butyl-2-cyclohexyloxy-benzamide], PA10 [5-amino-N-butyl-2-phenoxy-benzamide] and PA31 [5-arnino-N-butyl-2-(p-tolyloxy)-benzamide] was determined by an Ames Salmonella assay. The experiments were performed by preincubating
Publicado em: 2006
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16. Avaliação in vitro do potencial mutagênico de bidens pilosa Liné (picão - preto) e de Mikania glomerata Sprengel (guaco), por meio de ensaio do cometa e teste de micronúcleo
O consumo de Bidens pilosa Line (picao-preto) e de Mikania glomerata Sprengel (guaco) na forma de chas ocorre com freqüência na sociedade, principalmente em casos de ictericia e de afeccoes de vias aereas superiores, respectivamente. Para verificar a possível capacidade destas duas plantas de induzir danos ao DNA, foram utilizados o ensaio do cometa (SCGE
Publicado em: 2006
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17. Mutagenesis and in vitro technology in the genetic improvement of black pepper (Piper nigrum L.). / Mutagênese e tecnologia in vitro no melhoramento genético da pimenta-do-reino (Piper nigrum L.).
The purposes of the present work were to develop in vitro technology, associating it with mutagenesis, and to evaluate the V5 and V6 plants based on agronomical characters of production in Fusarium incidence areas, aiming at the genetic improvement of black pepper to obtain tolerant and/or resistant plants to the disease. The use of in vitro techniques start
Publicado em: 2003
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18. In vitro scanning saturation mutagenesis of an antibody binding pocket
We have combined PCR mutagenesis with in vitro transcription/translation and ELISA for the rapid generation and characterization of antibody mutants. The PCR products are used directly as the template for the in vitro transcription/translation reactions and because no cloning steps are required, the in vitro saturation mutagenesis of one residue can be compl
The National Academy of Sciences of the USA.
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19. Mutagenesis of bacteriophage T7 and T7 DNA by alkylation damage.
We have developed a new assay for in vitro mutagenesis of bacteriophage T7 DNA that measures the generation of mutations in the specific T7 gene that codes for the phage ligase. This assay was used to examine mutagenesis caused by in vitro DNA synthesis in the presence of O6-methylguanosine triphosphate. Reversion of one of the newly generated ligase mutants
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20. Mutagenesis of Neisseria meningitidis by In Vitro Transposition of Himar1 mariner
Now that the meningococcal genome sequence has been completed, the lack of a suitable method for saturation mutagenesis remains a major obstacle to the unraveling of the pathogenic propensity of Neisseria meningitidis. Here, we demonstrate that in vitro Himar1 mariner transposition on chromosomal or PCR-amplified meningococcal DNA, which is subsequently rein
American Society for Microbiology.
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21. Efficient site directed in vitro mutagenesis using ampicillin selection.
A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampici
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22. Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.
An in vitro system in which bacteriophage T7 DNA is replicated and efficiently packaged into procapsids to form viable phage has been used to examine mutagenesis. The fidelity of replication was assayed both by measuring reversion of an amber mutation in an essential gene and by generation of temperature-sensitive mutants among the phage produced in vitro. U
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23. Solid phase in vitro mutagenesis using plasmid DNA template.
Site-specific mutagenesis was accomplished using a solid support to generate single stranded vector and insert fragments which can be used to form gap-duplex plasmids through flanking, complementary double stranded regions. More than 80% mutants were obtained in both a single and a double primer approach. No special vectors or strains are needed and mismatch
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24. Biochemical basis of SOS-induced mutagenesis in Escherichia coli: Reconstitution of in vitro lesion bypass dependent on the UmuD′2C mutagenic complex and RecA protein
Damage-induced SOS mutagenesis requiring the UmuD′C proteins occurs as part of the cells’ global response to DNA damage. In vitro studies on the biochemical basis of SOS mutagenesis have been hampered by difficulties in obtaining biologically active UmuC protein, which, when overproduced, is insoluble in aqueous solution. We have circumvented this proble
The National Academy of Sciences.