Immobilized Polymers
Mostrando 13-24 de 25 artigos, teses e dissertações.
-
13. Interaction between biomolecules and solid surfaces / Interação entre a enzima enolase e superfícies sólidas
This work aimed to compare the adsorption behavior of enolase (2-phospho-D- glycerate hydrolase) onto hydrophilic (silicon wafers and amino-terminated surfaces (APS)) and hydrophobic planar substrates (polystyrene (PS) film, TMCS). The effect of the substrate shape (planar x spherical) was also studied. The spherical substrates were glass beads, native and m
Publicado em: 2004
-
14. Comportamento de reator anaeróbio operado em batelada seqüencial, contendo biomassa imobilizada e submetido a aumento progressivo da concentração de substrato de fácil degradação / Behavior of the anaerobic sequencing batch reactor containing immobilized biomass and submitted to a progressive increasing of the concetration of easy degradation substrate
The main objectives of this study were to evaluate the performance of the anaerobic sequencing batch reactor when subjected to a progressive increasing of the influent glucose concentration and estimate the kinetic parameters of glucoses degradation. Initially the reactor was operated with 8-hour cycles, treating glucose at concentrations of 500, 1000 and 20
Publicado em: 2003
-
15. Imobilização de horseradish peroxidase em diferentes polianilinas
This work describes peroxidase immobilization on chemically synthesized polyaniline activated with polyglutaraldehyde. Five polyanilines with different ranges of oxidation, counter-íons and levels of ring substitution were synthesized and characterized. The polymers were then activated with polyglutaraldehyde to chemically bind the peroxidase. The best enzy
Publicado em: 2000
-
16. Otimização por planejamento experimental da imobilização de lipase em silica de porosidade controlada na presença de estabilizantes
A metodologia de preparação do biocatalisador pode influenciar no processo catalítico. De acordo com estudos anteriores a lipase de Candida rugosa pode ser imobilizada com presença de alta atividade em sílica de porosidade controlada (SPC) ativada com glutaraldeído. Neste trabalho, foram desenvolvidas estratégias para otimizar a estabilidade operacion
Publicado em: 2000
-
17. Avaliação da radiação gama como agente de imobilização de algumas fases estacionarias sobre suportes para uso em cromatografia gasosa
The liquid stationary phases used in gas chromatography present a serious limitation; their volatility and/ or thermal stability which restrict their use to a specified temperature range. In an attempt to minimize this kind of problem, gamma radiation, in different doses, was used to effectuate immobilization of some liquid stationary phases on supports for
Publicado em: 1989
-
18. Biological activity of catecholamines covalently linked to synthetic polymers: Proof of immobilized drug theory
-
19. Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.
Synthetically-prepared 5'-NH2-dT(pdT)n oligomers (66,n=4 or 7) were immobilized on cyanogen bromide activated cellulose. The influence of temperature, pH, and ionic strength on the rate of the coupling process was studied. The oligomer 5'-NH2-DT(pdT)8 could be elongated enzymatically to the polymers 5'-NH2-dT(pdT)n (n=20, 51 and 84), which could be immobiliz
-
20. Photoresponsive polymer–enzyme switches
The ability to photoregulate enzyme activities could provide important new opportunities for development of diagnostic assays, sequential bioprocessing, and lab assays in both traditional and microfluidic formats. We show here that the photoinduced changes in the size and hydration of a “smart” polymer chain coil can be used to regulate substrate access
National Academy of Sciences.
-
21. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
Biotin-labelled DNA probes, prepared by nick-translation in the presence of biotinylated analogs of TTP, are hybridized to DNA or RNA immobilized on nitrocellulose filters. After removal of residual probe, the filters are incubated for 2--5 min with a preformed complex made with avidin-DH (or streptavidin) and biotinylated polymers of intestinal alkaline pho
-
22. Trapping of branched DNA in microfabricated structures.
We have observed electrostatic trapping of tribranched DNA molecules undergoing electrophoresis in a microfabricated pseudo-two-dimensional array of posts. Trapping occurs in a unique transport regimen in which the electrophoretic mobility is extremely sensitive to polymer topology. The arrest of branched polymers is explained by considering their center-of-
-
23. Selective adhesion of functional microtubules to patterned silane surfaces.
We show that microtubule polymers can be immobilized selectively on lithographically patterned silane surfaces while retaining their native properties. Silane films were chemisorbed on polished silicon wafers or glass coverslips and patterned using a deep UV lithographic process developed at the Naval Research Laboratory. Hydrocarbon and fluorocarbon alkyl s
-
24. Biological activity of catecholamines covalently linked to synthetic polymers: proof of immobilized drug theory.
l-Isoproterenol was covalently coupled via an azo linkage to soluble copolypeptides of molecular weight 1500 and 10,000 containing an aromatic amine. The polymeric azo-isoproterenol derivatives were purified by gel chromatography which reduced contamination by the parent isoproterenol to undetectable levels (i.e., less than 0.01%) and by 6-aminoisoproterenol