Hydrolases
Mostrando 13-24 de 419 artigos, teses e dissertações.
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13. Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell
Brazilian Journal of Microbiology. Publicado em: 2012-03
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14. Diurnal changes in storage carbohydrate metabolism in cotyledons of the tropical tree Hymenaea courbaril L. (Leguminosae)
The cotyledons of Hymenaea courbaril store large amounts of xyloglucan, a cell wall polysaccharide that is believed to serve as storage for the period of seedling establishment. During storage mobilisation, xyloglucan seems to be degraded by a continuous process that starts right after radicle protrusion and follows up to the establishment of photosynthesis.
Braz. J. Bot.. Publicado em: 2012
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15. Construção de biblioteca metagenômica de DNA microbiano do solo amazônico e prospecção de enzimas de interesse biotecnológico
Problemas relacionados a diminuicao dos estoques de combustiveis fosseis, politicos e ambientais tem levado a busca de alternativas renovaveis. A biomassa vegetal e abundante e barata, sendo candidata ideal para producao de biocombustiveis; entanto, o material lignocelulosico presente na parede celular das plantas possui estrutura complexa e atualmente nao e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/06/2011
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16. Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk
The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High le
Braz. J. Microbiol.. Publicado em: 2011-12
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17. Caracterização bioquímica e molecular da ß-Galactosidade durante a maturação de frutos de coffea arabica
ß-galactosidases are a class of glycosyl-hydrolases that act on the plant cell primary walls, hydrolyzing ß-D-galactose at the nonreducing ends of ß-D-galactosides present in several biological molecules. Initially a characterization of the monosaccharides present in the primary wall of the pericarp and endosperm of Coffea arabica fruits at different ripe
Publicado em: 2011
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18. Modificações da parede celular de frutos do mamoeiro (Carica papaya L.) em diferentes estadios do desenvolvimento / Modifications of the cell wall of fruits of papaya (Carica papaya L.) at various stages of development
The plant cell wall is a unique component of plant tissues and its polysaccharide composition essential to understand food texture and its changes during post-harvestingl especially of climateric fruits, as is the case of papaya. The plant cell wall is composed of three domains: the first is formed by cellulose and hemicelluloses, the second of pectins and t
Publicado em: 2009
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19. Prospecção de genes de interesse biotecnológico : uma abordagem metagenômica
The total number of prokaryotic cells on Earth has been estimated at 4 to 6x1030 and only about 1% of microorganisms present in the environment can be cultivated by standard techniques of cultivation and plating. Therefore, it is a huge biological and genetic pool that can be exploited, for the identification and characterization of genes with biotechnologic
Publicado em: 2009
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20. Molecular bases of the specificity substrate of a β-glicodase / Bases moleculares da especificidade pelo substrato em β-glicosidases
The β-glycosidases of family 1 of the glycoside hydrolases (GH 1) are one of the most important groups of enzymes. These enzymes are involved in a high diversity of physiological functions. The main objective of this study was the analysis of the molecular bases of the specificity for substrate of a β-glycosidase from the larvae of Spodoptera frugi
Publicado em: 2009
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21. Biophysical and structural studies on aspartic proteases from pathogenic parasites. / Estudos biofísicos e estruturais de aspartil proteases de parasitas patogênicos.
The purpose of this study is to compare the structure of aspartic proteases of Plasmodium falciparum and Schistosoma mansoni in different chemical environments in order to provide relevant information for future rational design of new drugs and new knowledge about the folding and dynamism of structural proteins. For this, changes in secondary and tertiary st
Publicado em: 2008
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22. Expressão heteróloga do gene Hxyn2 do fungo Humicola grisea var. thermoidea em Pichia pastoris: Produção e purificação da enzima HXYN2r e aplicação em testes de panificação / Heterologous expression of the gene Hxyn2 fungus Humicola grisea var. thermoidea in Pichia pastoris: production and purification of the enzyme HXYN2r testing and application in bakery
Endoxylanases are the main group of enzymes involved in the hydrolysis of xylan. This enzymes have application in industrial proposes, like as drink, food, feed, clothes industries and for bleaching cellulose paper pulps. In bread making the xylanases have been used to improve processing and product quality of loaf, leading soft dough and loaf with larger vo
Publicado em: 2008
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23. Analise filogenetica das enzimas hidroliticas de Xiloglucano no reino Viridiplantae e construção de bibliotecas de cDNA de Jatoba (Hymenaea courbaril) / Phylogenetic analysis of Xyloglucan s hydrolytic enzymes in the Viridiplantae kingdom and construction of cDNA libraries from Jatoba (Hymenaea courbaril)
Introdução: Os xiloglucanos são os polímeros de açúcar mais abundantes na hemicelulose da maioria das espécies de plantas terrestres, em especial nas eudicotiledôneas. Possuem papel estrutural na parede celular vegetal, interagindo com os filamentos de celulose, e podem ser utilizados como reserva em sementes de várias espécies de eudicotiledôneas
Publicado em: 2008
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24. Structural and functional studies of two glycosyl hydrolases : the putaitve cellulase XF0810 of Xylella fastidiosa and the digestive lysozyme 1 of Musca domestica / Estudos estruturais e funcionais de duas glicosideo hidrolases : a celulase putativa XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica
Glicosídeo hidrolases (EC 3.2.1.-) são enzimas que hidrolisam ligações glicosídicas. Neste trabalho realizamos estudos funcionais e estruturais de duas glicosídeo hidrolases: a celulase XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica (MdL1). A celulase XF0810 está anotada no genoma da X. fastidiosa como membro da família 5 da
Publicado em: 2007