Human Whole Blood
Mostrando 1-12 de 495 artigos, teses e dissertações.
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1. In vitro genotoxicity of nitroimidazoles as a tool in the search of new trypanocidal agents
BACKGROUND Only benznidazole (Bnz) (1) and nifurtimox (Nfx) (2) are licensed for the treatment of Chagas disease although their safety and efficacy profile are far from ideal. Farmanguinhos from Fiocruz has developed seven nitroimidazole compounds (4-10) analogs of megazol (3). OBJECTIVES To evaluate whether the genotoxic effect of 3 was abolished in the
Mem. Inst. Oswaldo Cruz. Publicado em: 27/06/2019
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2. Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors
ABSTRACT Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors. Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stor
Braz J Infect Dis. Publicado em: 20/05/2019
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3. Diagnosis of canine leptospirosis: evaluation of two PCR assays in comparison with the microagglutination test
RESUMO: O diagnóstico definitivo da leptospirose canina é geralmente realizado demonstrando a seroconversão em amostras do paciente no período agudo e de convalescença por serologia. No entanto, a aplicação de técnicas de PCR pode contribuir para a confirmação de casos suspeitos num período de tempo mais curto. O objetivo deste estudo foi avaliar
Pesq. Vet. Bras.. Publicado em: 02/05/2019
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4. Ustekinumab treats psoriasis by suppressing RORC and T-box but its suppression of GATA restrains its efficacy
Psoriasis is a T-cell mediated disease that involves IL-23/Th17 and IL-12/Th1 axes. Ustekinumab, a fully human monoclonal antibody targeting the p40 subunit of both IL-12 and IL-23, has proven to be efficient and safe for treating patients with psoriasis. Yet, there have been no reports with human skin/blood samples that would elucidate the molecular mechani
Braz. J. Pharm. Sci.. Publicado em: 08/04/2019
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5. Telomere length and hTERT in mania and subsequent remission
Objective: The findings of telomere length (TL) studies in bipolar disorder (BD) are controversial. The aim of the present study was to detect TL, human telomerase reverse transcriptase (hTERT), and brain derived neurotrophic factor (BDNF) in severe mania and subsequent remission. Methods: Twenty-one medication-free male patients and 20 age and gender matc
Rev. Bras. Psiquiatr.. Publicado em: 06/07/2017
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6. Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols
Abstract Saliva when compared to blood collection has the following advantages: it requires no specialized personnel for collection, allows for remote collection by the patient, is painless, well accepted by participants, has decreased risks of disease transmission, does not clot, can be frozen before DNA extraction and possibly has a longer storage time. O
J. Appl. Oral Sci.. Publicado em: 2017-04
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7. The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures
Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 5
Braz. arch. biol. technol.. Publicado em: 2015-02
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8. Detection of human cytomegalovirus DNA in various blood components after liver transplantation
The quantification of human cytomegalovirus (HCMV DNA) by real-time PCR is currently a primary option for laboratory diagnosis of HCMV infection. However, the optimal sample material remains controversial due to the use of different PCR assays. To explore the best blood component for HCMV DNA surveillance after liver transplantation, whole blood (WB), serum
Braz J Med Biol Res. Publicado em: 08/04/2014
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9. Megazol and its bioisostere 4H-1,2,4-triazole: comparing the trypanocidal, cytotoxic and genotoxic activities and their in vitro and in silico interactions with the Trypanosoma brucei nitroreductase enzyme
Megazol (7) is a 5-nitroimidazole that is highly active against Trypanosoma cruzi and Trypanosoma brucei, as well as drug-resistant forms of trypanosomiasis. Compound 7 is not used clinically due to its mutagenic and genotoxic properties, but has been largely used as a lead compound. Here, we compared the activity of 7 with its 4H-1,2,4-triazole bioisostere
Mem. Inst. Oswaldo Cruz. Publicado em: 18/03/2014
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10. Chagas disease: control, elimination and eradication. Is it possible?
From an epidemiological point of view, Chagas disease and its reservoirs and vectors can present the following characteristics: (i) enzooty, maintained by wild animals and vectors, with broad occurrence from southern United States of America (USA) to southern Argentina and Chile (42ºN 49ºS), (ii) anthropozoonosis, when man invades the wild ecotope and beco
Mem. Inst. Oswaldo Cruz. Publicado em: 06/12/2013
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11. Interleukin-10-dependent down-regulation of interferon-gamma response to Leishmania by Mycobacterium leprae antigens during the clinical course of a coinfection
We have described a case of a patient with an intriguing association of mucocutaneous leishmaniasis with lepromatous leprosy, two opposite polar forms of these spectral diseases. In the present follow-up study, we investigated the effect of the addition of Mycobacterium leprae antigens on interferon-gamma (IFN-γ) production in Leishmania antigen-stimulated
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-07
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12. Early HHV-6 replication is associated with morbidity non-related to CMV infection after kidney transplantation
Human herpesvirus type 6-(HHV-6) has been associated with morbidity after liver transplantation. OBJECTIVE: The aim of this study was to determine the HHV-6 seroprevalence among donor-recipient pairs, analyze the incidence of early active infection, its clinical manifestation, interaction with CMV, and the related morbidity in the first year after kidney tra
Brazilian Journal of Infectious Diseases. Publicado em: 2012-04