Hrgps
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Proteínas estruturais da parede celular do pólen de cinco espécies e sua relação com o boro
Boro (B) é um micronutriente essencial para a sobrevivência das plantas vasculares. O efeito mais drástico derivado da sua deficiência é a alteração na biogênese da parede celular durante a germinação de pólen. Investigamos pólen de espécies de plantas que requerem B para germinar (Zea may L. e Nicotiana tabacum L.), e de outras que podem germin
Brazilian Journal of Plant Physiology. Publicado em: 2005-12
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2. Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues.
Cell wall hydroxyproline-rich glycoproteins (HRGPs) and glycine-rich proteins (GRPs) were examined at the protein and at the mRNA levels in developing soybean tissues by tissue print immunoblots and RNA blots. In young soybean stems, HRGPs are expressed most heavily in cambium cells, in a few layers of cortex cells surrounding primary phloem, and in some par
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3. Synthetic genes for glycoprotein design and the elucidation of hydroxyproline-O-glycosylation codes
Design of hydroxyproline (Hyp)-rich glycoproteins (HRGPs) offers an approach for the structural and functional analysis of these wall components, which are broadly implicated in plant growth and development. HRGPs consist of multiple small repetitive “glycomodules” extensively O-glycosylated through the Hyp residues. The patterns of Hyp-O-glycosylation a
The National Academy of Sciences.
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4. Isolation and characterization of three soybean extensin cDNAs.
We have characterized three different soybean (Glycine max) mRNAs that encode apoproteins of extensins, a family of cell wall hydroxyproline-rich glycoproteins (HRGPs). These transcripts encoded distinctive Tyr-rich proteins containing characteristic Ser-Pro4 sequences organized in higher-order repetitive units. The first transcript encoded an extensin SbHRG
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5. Cell wall regeneration in Chlamydomonas: accumulation of mRNAs encoding cell wall hydroxyproline-rich glycoproteins.
The unicellular alga Chlamydomonas reinhardtii is surrounded by a cell wall composed entirely of hydroxyproline-rich glycoproteins (HRGPs). When the walls of vegetative cells are removed with the enzyme gamete autolysin (g-lysin), they regenerate a matrix within 3-4 hr. In vitro translation of mRNAs isolated from g-lysin-treated cells showed significant incr
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6. Purification and Partial Characterization of a Hydroxyproline-Rich Glycoprotein in a Graminaceous Monocot, Zea mays1
Graminaceous monocots generally contain low levels of hydroxyproline-rich Glycoproteins (HRGPs). As HRGPs are often at the cell surface, we used the intact cell elution technique (100 millimolar AlCl3) to isolate soluble surface proteins from Zea mays cell suspension cultures. Further fractionation of the trichloroacetic acid-soluble eluate on the cation exc
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7. Developmental regulation and phytochrome-mediated induction of mRNAs encoding a proline-rich protein, glycine-rich proteins, and hydroxyproline-rich glycoproteins in Phaseolus vulgaris L.
We have studied developmental and light regulation of mRNAs encoding a putative cell wall proline-rich protein (PvPRP1), cell wall glycine-rich proteins (GRPs), and cell wall hydroxyproline-rich glycoproteins (HRGPs) in bean (Phaseolus vulgaris). Light increases the levels of these mRNAs 2- to 150-fold in highly spatially regulated patterns during seedling d
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8. Glycosylation Motifs That Direct Arabinogalactan Addition to Arabinogalactan-Proteins1
Hydroxyproline (Hyp)-rich glycoproteins (HRGPs) participate in all aspects of plant growth and development. HRGPs are generally highly O-glycosylated through the Hyp residues, which means carbohydrates help define the interactive molecular surface and, hence, HRGP function. The Hyp contiguity hypothesis predicts that contiguous Hyp residues are sites of
The American Society for Plant Biologists.
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9. Molecular characterization of a zygote wall protein: an extensin-like molecule in Chlamydomonas reinhardtii.
The green alga Chlamydomonas reinhardtii elaborates two biochemically and morphologically distinct cell walls during its life cycle: one surrounds the vegetative and gametic cell and the other encompasses the zygote. Hydroxyproline-rich glycoproteins (HRGPs) constitute a major component of both walls. We describe the isolation and characterization of a zygot
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10. Isodityrosine cross-linking mediates insolubilization of cell walls in Chlamydomonas.
Enzymatic removal of the cell wall induces vegetative Chlamydomonas reinhardtii cells to transcribe wall genes and synthesize new hydroxyproline-rich glycoproteins (HRGPs) related to the extensins found in higher plant cell walls. A cDNA expression library made from such induced cells was screened with antibodies to an oligopeptide containing the (SP)x repet
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11. Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection
Hydroxyproline-rich glycoproteins (HRGPs) are important structural components of plant cell walls and also accumulate in response to infection as an apparent defense mechanism. Accumulation of HRGP mRNA in biologically stressed bean (Phaseolus vulgaris L.) cells was monitored by blot hybridization with 32P-labeled tomato genomic HRGP sequences. Elicitor trea
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12. Plus and Minus Sexual Agglutinins from Chlamydomonas reinhardtiiW⃞
Gametes of the unicellular green alga Chlamydomonas reinhardtii undergo sexual adhesion via enormous chimeric Hyp-rich glycoproteins (HRGPs), the plus and minus sexual agglutinins, that are displayed on their flagellar membrane surfaces. We have previously purified the agglutinins and analyzed their structural organization using electron microscopy. We repor
American Society of Plant Biologists.