Homologous Recombination
Mostrando 1-12 de 2585 artigos, teses e dissertações.
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1. Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model
Abstract The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumu
Genet. Mol. Biol.. Publicado em: 13/12/2019
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2. Reviewing the characteristics of BRCA and PALB2-related cancers in the precision medicine era
Abstract Germline mutations in BRCA1 and BRCA2 (BRCA) genes confer high risk of developing cancer, especially breast and ovarian tumors. Since the cloning of these tumor suppressor genes over two decades ago, a significant amount of research has been done. Most recently, monoallelic loss-of-function mutations in PALB2 have also been shown to increase the ris
Genet. Mol. Biol.. Publicado em: 29/04/2019
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3. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were
Braz. J. Microbiol.. Publicado em: 2016-12
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4. Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field
Mem. Inst. Oswaldo Cruz. Publicado em: 17/07/2015
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5. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro l
Braz. J. Microbiol.. Publicado em: 2014-12
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6. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility
The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. The homozygous Znf230
Genet. Mol. Biol.. Publicado em: 21/10/2014
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7. Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster
The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate - EMS and mitomycin C - MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART)
Genet. Mol. Biol.. Publicado em: 2014
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8. Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Dengue virulence and fitness are important factors that determine disease outcome. However, dengue virus (DENV) molecular biology and pathogenesis are not completely elucidated. New insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. Unfortunately, instability of flavivirus genomes cloned in E
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2013
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9. Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique
O replicon de RNA derivado do genoma de Flavivirus é uma ferramenta valiosa para o estudo de replicação viral independente da montagem e da maturação do virion, além de possuir um grande potencial para expressão de genes heterólogos. Neste estudo nós descrevemos a construção de replicons subgenômicos do vírus da febre amarela utilizando a técni
An. Acad. Bras. Ciênc.. Publicado em: 01/03/2013
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10. CONSTRUÇÃO E MANIPULAÇÃO DE CLONE INFECCIOSO DE UMA AMOSTRA BRASILEIRA DO VÍRUS DA DIARREIA VIRAL BOVINA / CONSTRUCTION AND MANIPULATION OF INFECTIOUS CLONE FROM A BRAZILIAN BOVINE VIRAL DIARRHEA VIRUS ISOLATE
O vírus da diarreia viral bovina (BVDV) é um patógeno de bovinos distribuído mundialmente, associado com importantes perdas econômicas. As maiores perdas devem-se aos problemas reprodutivos causados pela infecção, e pela capacidade do vírus de causar persistência após infecção fetal no terço inicial da gestação. Para entender melhor a biologia
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/03/2012
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11. CARACTERIZAÇÃO MOLECULAR DOS COMPONENTES DO SISTEMA ANGIOTENSINA-(1-7) DURANTE A DIVERGÊNCIA FOLICULAR E EXPRESSÃO DE GENES DE REPARO DA FITA DUPLA DE DNA EM EMBRIÕES BOVINOS / MOLECULAR CHARACTERIZATION OF THE ANGIOTENSIN-(1-7) SYSTEM COMPONENTS DURING FOLLICULAR DEVIATION AND EXPRESSION OF DNA DOUBLE-STRANDED REPAIR GENES IN BOVINE EMBRYOS
The first study characterized the expression of MAS receptor and key enzymes for Ang-(1-7) production, such as, ACE2, NEP and PEP during follicular development. Furthermore, the regulation of local Ang1-7 system was evaluated after the intrafollicular injection of fulvestrant (an estradiolreceptor inhibitor) in the dominant follicle. Cows were ovariectomized
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2012
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12. Assessment of genetic mutations in the XRCC2 coding region by high resolution melting curve analysis and the risk of differentiated thyroid carcinoma in Iran
Homologous recombination (HR) is the major pathway for repairing double strand breaks (DSBs) in eukaryotes and XRCC2 is an essential component of the HR repair machinery. To evaluate the potential role of mutations in gene repair by HR in individuals susceptible to differentiated thyroid carcinoma (DTC) we used high resolution melting (HRM) analysis, a recen
Genetics and Molecular Biology. Publicado em: 02/02/2012