Heterologous Primers
Mostrando 1-12 de 63 artigos, teses e dissertações.
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1. Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Arioca
Genet. Mol. Biol.. Publicado em: 21/02/2019
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2. Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping.
Carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In thi
Theoretical and Applied Genetics. Publicado em: 2011
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3. Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically des
Genetics and Molecular Biology. Publicado em: 12/03/2010
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4. Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the
Genetics and Molecular Biology. Publicado em: 22/01/2010
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5. Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the
Publicado em: 2010
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6. Caracterização molecular de rotavírus genotipo P[6] de origem humana em um foco de diarreia neonatal suína
Neonatal diarrhea is the major piglet health problem in swine production systems worldwide.In intensive production the porcine serogroup A rotaviruses (PoRV-A) are common cause of diarrhea in suckling and recently weaned piglets. The VP4 and VP7 proteins of the outer layer viral capsid of RV-A are relevant for immunity against rotavirus infection by inducing
Publicado em: 2010
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7. Aplicação de marcadores microssatélites na caracterização de recursos genéticos de Tabebuia roseo-alba conservados ex situ no banco de Germoplasma da floresta da USP de Ribeirão Preto / Application of microsatellite markers in the genetic resources characterization of Tabebuia roseo-alba conserved ex situ at the Germplasm Bank of the USP Forest in Ribeirão Preto State of Sao Paulo, Brazil
O interior do Estado de São Paulo, anteriormente ocupado por matas semidecíduas e cerrado, hoje está praticamente tomado por diferentes culturas ou pastagens, restando apenas algumas pequenas manchas de cerrado e de mata, apontando para uma drástica perda do rico patrimônio genético florestal. A região de Ribeirão Preto é uma das mais devastadas do
Publicado em: 2008
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8. EXPRESSÃO GÊNICA DIFERENCIAL EM TAMBAQUIS, Colossoma macropomum (Cuvier, 1818), EXPOSTOS AO PETRÓLEO E A HIPOXIA.
Petroleum industry is a reality in the Amazon region. Specific sediment and pH, along with water level changes, which induce specific patterns of oxygenation and floating vegetation, make such a complex environment that becomes difficult to predict all impacts of an oil spill in the Amazon basin. The present work investigates the effects of petroleum exposur
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/09/2007
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9. Heterologous microsatellite primer pairs informative for the whole genus Arachis
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of
Genetics and Molecular Biology. Publicado em: 2006
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10. Detection of animal and human group B rotaviruses in fecal specimens by polymerase chain reaction.
A combined reverse transcriptase reaction-polymerase chain reaction (RT-PCR) was developed to achieve the sensitive detection of group B rotaviruses (GBR). Sequences derived from genomic segment 3 of the IDIR (intestinal disease of infant rats) strain of GBR permitted the detection of greater than or equal to 0.08 pg of purified IDIR genomic RNA (4,000 genom
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11. One-sided polymerase chain reaction: the amplification of cDNA.
We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific primers from only one side of the region to be amplified. Th
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12. Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients.
Oligonucleotide primers based on Borrelia burgdorferi sensu lato ospA gene sequences have been designed for use in the PCR to type all (SL primers) or each (GI to GIII primers) of the B. burgdorferi sensu lato genospecies involved in Lyme disease. These genospecies-specific primers were then used in the PCR on 24 biological fluids collected from 18 neuroborr