Gppp
Mostrando 1-12 de 112 artigos, teses e dissertações.
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1. Testando a Teoria da Paridade do Poder de Compra Generalizada no continente latino-americano
O trabalho desenvolvido tem como objetivo principal testar a teoria da Paridade do Poder de Compra Generalizada (PPCG) para um conjunto de países latino-americanos. O teste consiste em verificar se existe ao menos um vetor de cointegração entre as taxas de câmbio real de um conjunto de países. Os testes de raiz unitária ADF, Phillips-Perron e ADF-GLS f
Publicado em: 2007
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2. A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.
Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present in many eukaryotic mRNAs. The cap binding protein does not bind the unmethylated co
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3. Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-α
We screened a human cDNA library for proteins that bind mRNA cap methyltransferase (MT) and isolated nuclear transporter importin-α (Impα). This direct association was confirmed by glutathione S-transferase (GST) pulldown, coimmunoprecipitation, and nuclear colocalization. In gel shift assays, MT selectively bound RNA containing 5′-terminal GpppG, and bi
Cold Spring Harbor Laboratory Press.
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4. Simian virus 40 early mRNA's in lytically infected and transformed cells contain six 5'-terminal caps.
Late simian virus 40 (SV40) mRNA contains eight different cap structures which we have previously identified and mapped on the viral genome. As reported here, 5'-cap heterogeneity is a common feature to both the early and the late SV40 mRNA's. methyl-3H-labeled viral mRNA was purified from cells infected at 41 degrees C with SV40 mutant tsA209. Three differe
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5. Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
Viral mRNA synthesized in vitro by reovirusassociated enzymes contained 5'-terminal ppG, GpppG, or m7GpppGm. Selective synthesis of one type of 5'-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5'-terminal sequences of mRNA (capformation). The pr
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6. 5′-Terminal Sequences of Spring Viremia of Carp Virus RNA Synthesized In Vitro
Sequence analyses have been undertaken on the 5′ termini of the RNA species synthesized in vitro at 22°C by Spring viremia of carp virion (SVCV)-associated transcriptase by using virus grown in mammalian BHK-21 cells. SVCV product RNA was synthesized in the absence or presence of low (0.56 μM) or high (0.8 mM) concentrations of added S-adenosyl-l-methion
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7. Host range mutants of vesicular stomatitis virus defective in in vitro RNA methylation.
The viral RNA polymerase of detergent-treated vesicular stomatitis virus normally synthesizes viral mRNAs in vitro that are both guanylylated and methylated to give 5'-terminal 7mGpppAm caps. We have characterized a virus host range mutant, hr 1, that is totally defective in vitro in the methylation of mRNA, although full-length polyadenylylated mRNAs with 5
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8. Isolation and characterization of rabbit anti-m7G-5'-P antibodies of high apparent affinity.
Antibodies highly specific for intact pm7G (7methylguanosine-5'-mono-phosphate) were induced by immunization of rabbits with a pm7G-BSA conjugate. Since the nucleotide is six-fold more stable than m7G (7-methylguanosine) to alkali-catalyzed fission of the imidazole ring, it is a more desirable antigen for obtaining antibodies capable of binding caps on eukar
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9. The 5' termini of RNAs encoded by the transposable element copia.
The 5' termini of copia-specific RNAs in Drosophila melanogaster tissue culture cells were determined by S1 nuclease mapping and cap analysis. Both major copia RNAs share an identical set of heterogeneous 5' ends. Three major cap 1 structures M7GpppCmpUp, M7GpppCmpCp and M7GpppGmpUp together with several other minor caps were found. Almost all the 5' termini
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10. Nucleotide sequences and mapping of novel heterogenous 5'-termini of adenovirus 2 early region 4 mRNA.
The major 5'-termini of human adenovirus type 2 early gene block 4 mRNA were sequenced. Poly(A+) polyribosomal RNA was isolated from Ad2 early infected cells, the 5'-terminal m7GPPP removed and the 5'-OH of the penultimate 2'-0-methylated nucleotide labeled with [gamma-32P]ATP using polynucleotide kinase. Ad2 E4 mRNA was purified by hybridization to the Ad2
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11. Capping of Viral RNA in Cultured Spodoptera frugiperda Cells Infected with Autographa californica Nuclear Polyhedrosis Virus
Viral RNA from fall armyworm (Spodoptera frugiperda) cells infected with Autographa californica nuclear polyhedrosis virus contains cap structures. Most of the cap labeled in vivo with [3H]methionine or 32Pi cochromatographed on DEAE-cellulose with the −5 charge marker; a minor component appeared at −4 net charge. The former is probably a cap 1 structure
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12. Heterogeneity of the 5' terminus of hen ovalbumin messenger ribonucleic acid.
The 5'-terminal sequence of hen ovalbumin mRNA was investigated using a novel labeling method. Ovalbumin mRNA was purified by hybridization to complementary DNA coupled to cellulose. The mRNA thus purified was shown to be 97.9% pure by hybridization with plasmid DNA containing sequences to the messengers coding for conalbumin and ovomucoid, the next two most