Glycinin
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Evaluation of substantial equivalence and potential of allergenic reactions of soybean cultivars tolerant to the glyphosate herbicide / Avaliação de equivalência substancial e potencial de alergenicidade de cultivares de soja tolerantes ao herbicida glifosato
Os parâmetros de avaliação de segurança de alimentos geneticamente modificados fundamentam-se na comparação de equivalência substancial entre as variedades e pela inocuidade de proteínas da planta GM com as proteínas encontradas nas plantas convencionais. O objetivo deste trabalho foi avaliar a segurança alimentar de três cultivares de sojas genet
Publicado em: 2009
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2. Evaluation of protein and metalloprotein changes in soybean seed after genetic modification process / Avaliação das alterações proteicas e metaloproteicas em soja apos o processo de modificação genetica
This research work consisted of comparison of two kinds of soybeans [Glycine max (L.) Merrill], transgenic and non-transgenic, using proteomics and metalloproteomics changes. Firstly, soybeans proteins were extracted with an extraction buffer containing Tris-HCI (pH 8.8), KCI, DTT, PMSF and SDS, and then they were quantified by the Bradford method. In terms
Publicado em: 2007
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3. Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting
Genetics and Molecular Biology. Publicado em: 2006
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4. Absence of the A4 peptide in the G4 glycinin subunit of soybean cultivar Enrei is caused by a point mutation in the Gy4 gene
Functional properties of soy proteins for food are closely related to the composition of their storage protein subunits. Using base excision sequence scanning (BESS), we show that the absence of the A4 peptide in the G4 glycinin subunit of the soybean (Glycine max L.) cultivar Enrei was caused by the same point mutation in the Gy4 gene as previously reported
Genetics and Molecular Biology. Publicado em: 2005-09
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5. Comportamiento de glicinina, beta-conglicinina y alfa-amilasa en semillas de soja deterioradas y no deterioradas
The objective of this research was to study the behaviour of the storage proteins glycinin and beta-conglycinin and the alpha-amylase activity in artificially deteriorated and not deteriorated soybean [Glycine max (L.) Merr.] seeds of 10 cultivars. The seeds were submitted to two treatments: deteriorated by accelerated aging and not deteriorated seeds. The p
Pesquisa Agropecuária Brasileira. Publicado em: 2002-08
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6. Characterization of the glycinin gene family in soybean.
We characterized the structure, organization, and expression of genes that encode the soybean glycinins, a family of storage proteins synthesized exclusively in seeds during embryogenesis. Five genes encode the predominant glycinin subunits found in soybeans, and they have each been cloned, sequenced, and compared. The five genes have diverged into two subfa
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7. Differential Proteolysis of Glycinin and β-Conglycinin Polypeptides during Soybean Germination and Seedling Growth 1
The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and β-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting t
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8. Role of posttranslational cleavage in glycinin assembly.
Glycinin, like other 11S seed storage proteins, undergoes a complex series of posttranslational events between the time proglycinin precursors are synthesized in endoplasmic reticulum and the mature glycinin subunits are deposited in vacuolar protein bodies. According to the current understanding of this process, proglycinin subunits aggregate into trimers i
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9. The complete nucleotide sequence of soybean glycinin A2B1a gene spanning to another glycinin gene A1aB1b.
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10. A cDNA clone encoding a glycinin A1a subunit precursor of soybean.
A cDNA clone covering the whole coding region for a glycinin subunit precursor containing the A1a acidic subunit, one of the A2 family, has been identified from a library of soybean cotyledonary cDNA clones using a mixed oligonucleotide probe. Analysis of the cDNA insert revealed that it contained 1746 nucleotides of mRNA sequence with a 5'-terminal nontrans
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11. Accumulation of Soybean Glycinin and Its Assembly with the Glutelins in Rice1
Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processing of the coded protein. Wild-type and me
American Society of Plant Physiologists.
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12. Molecular characterization of an aberrant allele for the Gy3 glycinin gene: a chromosomal rearrangement.
The soybean variety Forrest contains an aberrant allele for the Gy3 glycinin gene. The aberrant allele is designated gy3 because mRNA for the G3 glycinin subunit is reduced to below detectable amounts in the seed. Molecular and genetic characterization of gy3 show it to be associated with a chromosomal rearrangement that causes the 5' halves and 3' halves of