Glucoamylase
Mostrando 1-12 de 104 artigos, teses e dissertações.
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1. Anti-Hyperglycemic Activities, Molecular Docking and Structure-Activity Relationships (SARs) Studies of Endiandric Acids and Kingianins from Endiandra kingiana
Diabetes has become a severe chronic disease worldwide with patients significantly increasing daily. Due to the side effects of insulin and oral hypoglycaemic agents employed in diabetes treatment, scientists are working hard to develop alternative approaches from natural plants that inhibit α-amylase and α-glucosidase. Consequently, by performing a phytoc
Journal of the Brazilian Chemical Society. Publicado em: 2022
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2. Cassava Pulp Enzymatic Hydrolysate as a Promising Feedstock for Ethanol Production
ABSTRACT The aim of this study was to produce bioethanol from enzymatic hydrolysates of cassava pulp, a by-product of cassava flour manufacturing, using an alcohol-tolerant Saccharomyces cerevisiae strain. First, the best operational condition of the starch hydrolysis process was determined through a complete factorial design (24), with triplicates at the ce
Braz. arch. biol. technol.. Publicado em: 10/12/2018
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3. Enzymatic hydrolysis optimization of sweet potato (Ipomoea batatas) peel using a statistical approach
In this work, two-step enzymatic hydrolysis of sweet potato peel was optimized. The effects of time, enzyme dose and temperature on glucose concentration were investigated. The Box-Behnken design was applied and a total of 17 experimental runs were generated for each step. For the liquefaction step, an ANOVA test showed the quadratic model obtained to be sig
Braz. J. Chem. Eng.. Publicado em: 2013-09
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4. Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1
The production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon
Braz. J. Microbiol.. Publicado em: 2013
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5. Fed batch enzymatic saccharification of food waste improves the sugar concentration in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae H058
The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration
Brazilian Archives of Biology and Technology. Publicado em: 2012-04
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6. Ethanol production from sorghum grains [Sorghum bicolor (L.) Moench]: evaluation of the enzymatic hydrolysis and the hydrolysate fermentability
The production of ethanol from sorghum grains was investigated in the present work. Initially, starch enzymatic hydrolysis was investigated using commercial α-amylase and glucoamylase, considering particle size, solid:liquid ratio and enzyme load as variables. The hydrolysate, in the best conditions (73 U of α-amylase/g grain and 1150 U glucoamylase/g grai
Brazilian Journal of Chemical Engineering. Publicado em: 2011-12
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7. Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum
Brazilian Archives of Biology and Technology. Publicado em: 2011-06
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8. Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation
O fungo termofílico Thermomyces lanuginosus TO3 foi isolado a partir de amostras de material de pilhas de compostagem, com base em sua capacidade de crescer em meio líquido contendo amido como única fonte de carbono, a 45 ºC, e produzir considerável quantidade de glucoamilase. A produção da enzima por fermentação submersa FSm foi alta, com um máxim
Brazilian Archives of Biology and Technology. Publicado em: 2008-08
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9. Construção de sistema que permite a ancoragem de proteína recombinante à superfície celular de levedura. / Construction of a system that allows anchoring of recombinant protein to the cell surface of yeast.
Cell surface display systems have being developed for expression of heterologous proteins anchored to the cell surface of microorganisms. Several applications of these systems have been reported, including employment as whole-cell biocatalysts, development of vaccines and cellular biosorvents. In this work it was developed a system that allows the anchoring
Publicado em: 2008
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10. PRODUÇÃO, PURIFICAÇÃO PARCIAL E CARACTERIZAÇÃO BIOQUÍMICA DE GLUCOAMILASE DE Aspergillus niger OBTIDA POR FERMENTAÇÃO EM ESTADO SÓLIDO
A glucoamilase, enzima normalmente produzida por fungos filamentosos, é uma das principais responsáveis pela hidrólise do amido para a formação de xarope de glucose, matéria-prima utilizada pela indústria de alimentos na produção de refrigerantes, sorvetes, molhos, pães e como fonte de carbono em fermentações. No presente trabalho, a glucoamilase
Publicado em: 2007
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11. Aproveitamento biotecnológico de resíduos agroindustriais na produção de glucoamilase
The Alto Vale do Itajaí region is a big producer of cassava flour for starch. The processing of the cassava flour generates residues which are usually disposed in the environment in an inadequate way, leading to an environmental impact. This way, the biotechnology proves itself to be an interesting alternative in transforming these residues, which still con
Publicado em: 2006
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12. A produção de resíduos agroindustriais para a produção de amiloglucosidade. Aspergillus awamori
RESUMO Muitos resíduos gerados na agroindústria podem ser utilizados como substrato para produção de enzimas. A amiloglucosidase (AMG) é uma enzima utilizada pela indústria de alimentos para a fabricação de xaropes de glucose. A produção de AMG por processos fermentativos pode ser feita com o uso do Aspergillus awamori, pelo fato de ser considerado
Publicado em: 2006