Genome Pyrosequencing
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents
Objective: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitant cocaine and crack dependence, and healthy controls. Meth
Braz. J. Psychiatry. Publicado em: 16/05/2019
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2. Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosom
Genet. Mol. Biol.. Publicado em: 21/08/2015
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3. Pirossequenciamento e análise comparativa de genomas do fitopatógeno Xylella fastidiosa / Pyrosequencing and comparative analysis of Xylella fastidiosa genomes
Xylella fastidiosa é uma bactéria Gram-negativa, do subgrupo das Gama-Proteobactérias, não-flagelada, que coloniza o xilema de diversas plantas cultivadas e silvestres, podendo ser causadora de doenças. Sua disseminação é feita por insetos conhecidos como cigarrinhas. Genomas de cepas de X. fastidiosa isoladas de distintos hospedeiros já foram seque
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/03/2012
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4. Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS)
Manipulating the mouse genome is a widespread technology with important applications in many biological fields ranging from cancer research to developmental biology. Likewise, correlations between copy number variations (CNVs) and human diseases are emerging. We have developed the reference-query pyrosequencing (RQPS) method, which is based on quantitative p
Cold Spring Harbor Laboratory Press.
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5. Pyrosequencing: A Simple Method for Accurate Genotyping
Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a tremendous amount of data available comes an explosion of genotyping methods. Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. It also has the ability to iden
MyJove Corporation.
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6. A Sensitive Method for Detecting Variation in Copy Numbers of Duplicated Genes
Gene duplications are common in the vertebrate genome, and duplicated loci often show a variation in copy number that may have important phenotypic effects. Here we describe a powerful method for quantification of duplicated copies based on pyrosequencing. A reliable quantification was obtained by amplification of the duplication break-point and a corres
Cold Spring Harbor Laboratory Press.
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7. A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic loci, could be used for direct sequencing, selective restri
Oxford University Press.
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8. Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strains
Incorporation of strain-specific synthetic DNA tags into yeast Saccharomyces cerevisiae gene-deletion strains has enabled identification of gene functions by massively parallel growth rate analysis. However, it is important to confirm the sequences of these tags, because mutations introduced during construction could lead to significant errors in hybridizati
National Academy of Sciences.
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9. Determination of Single-Nucleotide Polymorphisms by Real-time Pyrophosphate DNA Sequencing
The characterization of naturally occurring variations in the human genome has evoked an immense interest during recent years. Variations known as biallelic Single-Nucleotide Polymorphisms (SNPs) have become increasingly popular markers in molecular genetics because of their wide application both in evolutionary relationship studies and in the identification
Cold Spring Harbor Laboratory Press.