Fractionation Of Toxins
Mostrando 1-12 de 15 artigos, teses e dissertações.
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1. Inhibitory effects of Swietenia macrophylla on myotoxic phospholipases A2
Activity-guided fractionation of an ethanol-soluble extract of the leaves of Swietenia macrophylla King, Meliaceae, led to several fractions. As a result, sample Sm13-16, 23 had the most promising activity against phospholipases A2 (PLA2), Asp49 and Lys49 types. This fraction inhibited PLA2 activity of the Asp49 PLA2, when aggregated substrate was used. On t
Rev. bras. farmacogn.. Publicado em: 2013-12
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2. Novo método de fracionamento da peçonha do escorpião Tityus serrulatus e caracterização eletrofisiológica das toxinas Ts6 e Ts7 / New fractionation procedure of Tityus serrulatus venom and electrophysiological characterization of toxins Ts6 and Ts7
In Brazil, Tityus serrulatus (Ts) species is the responsible for the most scorpion accidents and also for the major incidence of accidents caused by venomous animals. About 16 different toxins of Ts venom have been listed so far, being Ts1 the major one. Among these toxins, the neurotoxins with action on sodium and potassium channels are the most interest in
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/06/2012
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3. Biochemical and biological characterization of toxins in the venom and mucus of the catfish Cathorops spixii. / Caracterização bioquímica e biológica de toxinas presentes na peçonha e no muco do bagre Cathorops spixii.
Of the venomous fish found in Brazil, catfish noteworthy for the number of accidents they cause. The objective of this study was to characterize the toxic compounds present in the venom and mucus. We obtained an average protein intake of 3,1 mg / mL (venom) and 1,4 mg / mL (mucus). The electrophoretic profile of venom and mucus has a few protein bands. Fract
Publicado em: 2009
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4. On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
Whole-body extracts in methanol were obtained from the starfish Stellaster equestris. The crude toxin was fractionated stepwise using diethylaminoethyl (DEAE) cellulose column chromatography. The crude toxin was lethal to male albino mice at a dose of 1.00 mL (containing 531.0 µg/mL protein) when injected intraperitoneally (IP) but the toxicity was abolishe
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2008
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5. Analise histologica do aparato venenifero e caracterização farmaco-bioquimica da peçonha de Vitalius dubius (Araneae, Theraphosidae) / Histological analysis of the venom apparatus and biochemical and pharmacological characterization of venom from Vitalius dubius (Araneae, Theraphosidae)
Vitalius dubius is a medium-sized, non-aggressive tarantula found in southeastern Brazil. In this work, we examined the histological organization of the venom apparatus of V. dubius and investigated some biochemical and pharmacological properties of this spider´s venom. The venom apparatus consisted of two chelicerae fitted with large fangs for prey immobil
Publicado em: 2008
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6. Estudo comparativo dos venenos de escorpiões tityus bahiensis e tityus serrulatus e serrulatus e de suas principais toxinas
T. bahiensis and T.serrulatus are the main scorpion species involved in envenomations in the Brazil, the latter being the more widely distributed. Clinically, envenomations by T. serrulatus is more serious than that by T. bahiensis. In this thesis, the lethal and neurotoxic activities of venoms from these two species were compared. Male Swiss withe mice (18-
Publicado em: 2000
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7. The Toxins of Helminthosporium maydis (Race T) 1: A Colorimetric Determination of the Toxins, Their Appearance in Culture and in Infected Plants
Host-specific toxins produced by Helminthosporium maydis, race T, are measured quantitatively by a chemical assay procedure involving reaction of the toxins with a sulfuric acidacetic anhydride reagent and measurement of the absorbance of the product at 330 nm. The assay was shown to measure total toxin concentrations after only limited fractionation of the
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8. Defective acidification of endosomes in Chinese hamster ovary cell mutants "cross-resistant" to toxins and viruses.
Like many physiological ligands, several viruses and toxins enter mammalian cells through receptor-mediated endocytosis. Once internalized, the nucleic acids of several viruses and the toxic subunit of diphtheria toxin gain access to the cytosol of the host cell through an acidic intracellular compartment. In this report, we present evidence that one class o
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9. Clostridium difficile Toxins Disrupt Epithelial Barrier Function by Altering Membrane Microdomain Localization of Tight Junction Proteins
The anaerobic bacterium Clostridium difficile is the etiologic agent of pseudomembranous colitis. C. difficile toxins TcdA and TcdB are UDP-glucosyltransferases that monoglucosylate and thereby inactivate the Rho family of GTPases (W. P. Ciesla, Jr., and D. A. Bobak, J. Biol. Chem. 273:16021–16026, 1998). We utilized purified reference toxins of C. diffici
American Society for Microbiology.
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10. Fractionation of Phenol Extracts from Brucella suis: Separation of Multiple Biologically Active Components
Brucella suis endotoxin prepared by a modification of Westphal's method was purified further by gel filtration and ion-exchange chromatography. The latter proved to be the most effective means of separating antigenic components associated with this fraction. All of the toxic activity was located in a single peak from carboxymethyl-Sephadex. Subsequent separa
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11. PATE, a gene expressed in prostate cancer, normal prostate, and testis, identified by a functional genomic approach
To identify target antigens for prostate cancer therapy, we have combined computer-based screening of the human expressed sequence tag database and experimental expression analysis to identify genes that are expressed in normal prostate and prostate cancer but not in essential human tissues. Using this approach, we identified a gene that is expressed specifi
The National Academy of Sciences.
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12. Comparison of toxin overlay and solid-phase binding assays to identify diverse CryIA(c) toxin-binding proteins in Heliothis virescens midgut.
The binding proteins, or receptors, for insecticidal Bacillus thuringiensis subsp. kurstaki delta-endotoxins are located in the brush border membranes of susceptible insect midguts. The interaction of one of these toxins, CryIA(c), with proteins isolated from Heliothis virescens larval midguts was investigated. To facilitate the identification of solubilized