Fosmid
Mostrando 1-12 de 27 artigos, teses e dissertações.
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1. Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen
ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat r
Braz. J. Microbiol.. Publicado em: 2017-12
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2. A broad pH range and processive chitinase from a metagenome library
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene nam
Braz J Med Biol Res. Publicado em: 05/01/2017
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3. Bioprospecção de genes envolvidos na síntese de biossurfactantes a apartir de microbiota de resevatórios de petróleo / Bioprospection of genes involved in the synthesis of biosurfactants from braziliam petroleum reservoirs microbial communities
Bioemulsifiers or biosurfactants are compounds produced by microorganisms which enhance the bioavailability of hydrophobic substances promoting the stabilization of substances with different polarities, like oil-in-water emulsion occurring in petroleum reservoirs. The samples used in this work were collected in the Potiguar Basin (RN, Brazil), and employed a
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/07/2010
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4. Prospecção da atividade de degradação de fenol em metagenoma microbiano originado de efluente de refinaria de petróleo / Prospection of phenol-degrading activity in microbial metagenome from petroleum refinery wastewater
Os efluentes de refinarias contêm vários compostos tóxicos, que podem provocar sérios danos aos seres vivos se não tratados previamente. Os fenóis são poluentes encontrados em efluentes de diversas indústrias, incluindo as refinarias de petróleo, e são conhecidos como um dos compostos mais tóxicos encontrados no meio ambiente. Portanto, há necess
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/06/2010
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5. Construção de biblioteca metagenômica e prospecção de genes para a síntese de polihidroxalcanoatos / Metagenomic library construction for PHA synthase screening
Os microrganismos constituem dois terços da diversidade biológica na Terra, no entanto, muitos deles não podem ser cultivados por técnicas tradicionais. Portanto, o acesso a esta diversidade tem sido feita através da utilização de técnicas independentes de cultivo. Diante deste panorama, a metagenômica apresenta-se como uma alternativa, pois dispens
Publicado em: 2009
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6. Fosmid-Based Physical Mapping of the Histoplasma capsulatum Genome
A fosmid library representing 10-fold coverage of the Histoplasma capsulatum G217B genome was used to construct a restriction-based physical map. The data obtained from three restriction endonuclease fingerprints, generated from each clone using BamHI, HindIII, and PstI endonucleases, were combined and used in FPC for automatic and manual contig assembly bui
Cold Spring Harbor Laboratory Press.
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7. Stable propagation of cosmid sized human DNA inserts in an F factor based vector.
Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E. coli F factor replicon (Fosmid). We have tested relative stability of human DNA inserts in Fosmids and in two
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8. Molecular Cloning, Sequence Analysis, and Heterologous Expression of the Phosphinothricin Tripeptide Biosynthetic Gene Cluster from Streptomyces viridochromogenes DSM 40736
A fosmid library from genomic DNA of Streptomyces viridochromogenes DSM 40736 was constructed and screened for the presence of genes known to be involved in the biosynthesis of phosphinothricin tripeptide (PTT). Eight positives were identified, one of which was able to confer PTT biosynthetic capability upon Streptomyces lividans after integration of the fos
American Society for Microbiology.
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9. Screening of a Fosmid Library of Marine Environmental Genomic DNA Fragments Reveals Four Clones Related to Members of the Order Planctomycetales
A fosmid library with inserts containing approximately 40 kb of marine bacterial DNA (J. L. Stein, T. L. Marsh, K. Y. Wu, H. Shizuya, and E. F. DeLong, J. Bacteriol. 178:591–599, 1996) yielded four clones with 16S rRNA genes from the order Planctomycetales. Three of the clones belong to the Pirellula group and one clone belongs to the Planctomyces group, b
American Society for Microbiology.
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10. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon.
One potential approach for characterizing uncultivated prokaryotes from natural assemblages involves genomic analysis of DNA fragments retrieved directly from naturally occurring microbial biomass. In this study, we sought to isolate large genomic fragments from a widely distributed and relatively abundant but as yet uncultivated group of prokaryotes, the pl
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11. Trans-chromosomal recombination within the Ig heavy chain switch region in B lymphocytes
Somatic DNA rearrangements in B lymphocytes, including V(D)J gene rearrangements and isotype switching, generally occur in cis, i. e., intrachromosomally. We showed previously, however, that 3 to 7% of IgA heavy chains have the VH and Cα regions encoded in trans. To determine whether the trans-association of VH and Cα occurred by trans-chromosomal recombin
The National Academy of Sciences.
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12. Identification of Methyl Coenzyme M Reductase A (mcrA) Genes Associated with Methane-Oxidizing Archaea
Phylogenetic and stable-isotope analyses implicated two methanogen-like archaeal groups, ANME-1 and ANME-2, as key participants in the process of anaerobic methane oxidation. Although nothing is known about anaerobic methane oxidation at the molecular level, the evolutionary relationship between methane-oxidizing archaea (MOA) and methanogenic archaea raises
American Society for Microbiology.