Forensic Genetic
Mostrando 13-24 de 29 artigos, teses e dissertações.
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13. Desempenho forense de microssatÃlites para a investigaÃÃo da origem de Cannabis sativa no Brasil e no Paraguai
The black market of Cannabis sativa (marijuana) in Brazil is basically supplied by illegal plantations from Paraguay, North and Northeastern Brazil. Without a well established intelligence service is difficult to state the right origin of large and small apprehensions. Any new technology that helps this task will be of inestimable value for the war against t
Publicado em: 2006
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14. DNA exam application in crime elucidation / Aplicação do exame de DNA na elucidação de crimes
The wide application of the DNA exam in paternity investigation led to the massive divulgation of its efficiency through the communication channels, earning it a reputation of infallible result, and jeopardizing the credit of older analytical methods. While focused by the media as the most supreme technique, several limitations were omitted regarding its use
Publicado em: 2005
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15. DNA extraction of human teeth submitted to high temperatures: evaluation of three methods . / "Avaliação de três métodos de extração de DNA de dentes humanos submetidos ao calor"
Biological remains of carbonized human bodies are usually not possible to use in forensic analysis. In these cases, teeth are usually selected since enamel, dentin and cement provides protection to the genetic material. The present study evaluates DNA extracted using three methods (organic, isopropilic alcohol and silica) of teeth submitted to different temp
Publicado em: 2004
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16. The SCA1 (Spinocerebellar ataxia type 1) and MJD (Machado-Joseph disease) CAG repeats in normal individuals: segregation analysis and allele frequencies
Spinocerebellar ataxia type 1 (SCA1) and Machado-Joseph disease (MJD/SCA3) are autosomal dominant neurodegenerative diseases caused by expansions of a CAG trinucleotide repeat in the SCA1 and MJD genes. These expanded sequences are unstable upon transmission, leading to an intergeneration increase in the number of repeats (dynamic mutation). The transmission
Genetics and Molecular Biology. Publicado em: 2003
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17. Characterization of new Schistosoma mansoni microsatellite loci in sequences obtained from public DNA databases and microsatellite enriched genomic libraries
In the last decade microsatellites have become one of the most useful genetic markers used in a large number of organisms due to their abundance and high level of polymorphism. Microsatellites have been used for individual identification, paternity tests, forensic studies and population genetics. Data on microsatellite abundance comes preferentially from mic
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-10
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18. Genetic structure of forensic populations.
DNA-based identification depends on the probability that two different individuals have the same phenotype, which is given by kinship theory. Together with the large and consistent body of evidence on human population structure, kinship theory provides a sound basis for forensic use of DNA markers.
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19. Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay.
DNA diagnostics, the detection of specific DNA sequences, will play an increasingly important role in medicine as the molecular basis of human disease is defined. Here, we demonstrate an automated, nonisotopic strategy for DNA diagnostics using amplification of target DNA segments by the polymerase chain reaction (PCR) and the discrimination of allelic seque
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20. Population genetics in the forensic DNA debate.
The use of matching variable number of tandem repeat (VNTR) profiles to link suspects with crimes is potentially very powerful, but it has been quite controversial. Initial debate over laboratory procedures has largely given way to debate over the statistical and population genetic issues involved in calculating the frequency of a profile for a random member
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21. Y Chromosome STR Haplotypes and the Genetic Structure of U.S. Populations of African, European, and Hispanic Ancestry
To investigate geographic structure within U.S. ethnic populations, we analyzed 1705 haplotypes on the basis of 9 short tandem repeat (STR) loci on the Y-chromosome from 9–11 groups each of African-Americans, European-Americans, and Hispanics. There were no significant differences in the distribution of Y-STR haplotypes among African-American groups, where
Cold Spring Harbor Laboratory Press.
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22. Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip
We describe a method for the discrimination of short tandem repeat (STR) alleles based on active microarray hybridization. An essential factor in this method is electronic hybridization of the target DNA, at high stringency, in <5 min. High stringency is critical to avoid slippage of hybrids along repeat tracts at allele-specific test sites in the array. The
Oxford University Press.
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23. Direct haplotype determination by double ARMS: specificity, sensitivity and genetic applications.
We have developed a novel double Amplification Refractory Mutation System (double ARMS) using a highly polymorphic region 5' to the human delta-globin gene as a model system. The double ARMS approach involves using two allele-specific ARMS primers simultaneously during DNA amplification by the polymerase chain reaction (PCR). The resulting system is highly s
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24. Kinship bioassay on hypervariable loci in blacks and Caucasians.
Four hypervariable loci were examined in DNA samples of American Blacks and Caucasians. Genetic diversity, measured by mean kinship within race, is 0.004 for a sliding window equal to twice the radius of coalescence of the autoradiographic bands. Kinship increases with the width of a window or bin, but it is an order of magnitude less than for blood groups a