Fluorescence Polarization Assay
Mostrando 1-12 de 27 artigos, teses e dissertações.
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1. Validação interlaboratorial do teste de polarização fluorescente para o diagnóstico sorológico da brucelose bovina = Interlaboratorial validation of the fluorescence polarization assay for the serodiagnosis of bovine brucellosis
Esta investigação teve por objetivo validar o teste de polarização fluorescente (TPF) para o diagnóstico sorológico da brucelose bovina, determinando a sensibilidade (SE) e a especificidade relativas (SP) e verificando a reprodutibilidade do teste em quatro laboratórios no Brasil. Foram selecionadas 1.389 amostras de soro sanguíneo, as quais foram in
Publicado em: 2011
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2. Evaluation of an automated fluorescence polarization immunoassay for vancomycin.
An automated fluorescence polarization immunoassay for the determination of vancomycin levels in serum was evaluated. The vancomycin assay is a homogeneous competitive inhibition immunoassay based on changes in fluorescence polarization that occur with antibody binding. This assay was compared with a liquid chromatographic assay and an agar well diffusion bi
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3. Comparison of fluorescence polarization immunoassay and bioassay of vancomycin.
Human serum samples were analyzed for vancomycin concentrations by two different methods: the fluorescence polarization immunoassay and the disk plate bioassay. Each assay method offered acceptable precision. The correlation between both assay methods was excellent (correlation coefficient = 0.985). Excluding technical time, the bioassay was the least expens
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4. Fluorescence Polarization Assay for Detection of Brucella abortus Antibodies in Bulk Tank Bovine Milk Samples
A simple, rapid, inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and 95.9%, respectively, is described. The assay detects antibodies to B. abortus in 15 min by testing undiluted whey produced by chemical and phys
American Society for Microbiology.
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5. Automated fluorescence polarization immunoassay for monitoring streptomycin.
The fluorescence polarization immunoassay technique has been used previously for the assay of vancomycin and the aminoglycoside antibiotics (gentamicin, tobramycin, and amikacin). We extended this technique to assay streptomycin. Fluorescein-labeled streptomycin was used as the tracer, and antiserum specific for streptomycin was raised in rabbits by conventi
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6. Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.
The principle of fluorescence polarization described by Perrin (F. Perrin, J. Phys. Radium 7:390-401, 1926) was applied to the development of a novel assay that used fluorescein-labeled Mycobacterium bovis secretory protein MPB70 for rapid detection of anti-MPB70 antibodies in selected sera from three M. bovis-infected species (elk, Ilama, and bison). Labeli
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7. Fluorescence Polarization in Homogeneous Nucleic Acid Analysis II: 5′-Nuclease Assay
When the temperature and viscosity of the solvent is held constant, the degree of fluorescence polarization (FP) detected when a fluorescent dye is excited by plane polarized light depends mostly on the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye molecule, one can detect significant changes in the molecular weight of a flu
Cold Spring Harbor Laboratory Press.
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8. Comparison of enzyme-multiplied immunoassay technique with fluorescence polarization immunoassay for determination of gentamicin and tobramycin levels in serum.
We assayed serum gentamicin and tobramycin specimens by the enzyme multiplied immunoassay technique (Syva EMIT) and the fluorescence polarization immunoassay (Abbott TDx). When interassay and intraassay control samples were evaluated, both methods gave an overall coefficient of variation of less than +/- 10%. Using patient serum samples, we obtained excellen
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9. Simultaneously monitoring DNA binding and helicase-catalyzed DNA unwinding by fluorescence polarization
A new method for helicase-catalyzed DNA unwinding is described. This assay takes advantage of the substantial change in fluorescence polarization (FP) upon helicase binding and DNA unwinding. The low anisotropy value, due to the fast tumbling of the free oligonucleotide in solution, increases abruptly upon binding of helicase to the fluorescein-labeled oligo
Oxford University Press.
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10. Laboratory evaluation of five assay methods for vancomycin: bioassay, high-pressure liquid chromatography, fluorescence polarization immunoassay, radioimmunoassay, and fluorescence immunoassay.
We compared the precision and accuracy of five methods used to measure the concentration of vancomycin in serum: bioassay, high-pressure liquid chromatography, fluorescence polarization immunoassay (FPIA [TDX; Abbott Laboratories, North Chicago, Ill.]), radioimmunoassay (RIA), and fluorescence immunoassay. Based on an analysis of seven standards and of 106 p
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11. Exo-Proofreading, A Versatile SNP Scoring Technology
We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3′-to-5′ exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3′ nucleotide base, and can be implemented in a variety of formats including a one-step PCR reaction that allows SNP t
Cold Spring Harbor Laboratory Press.
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12. Myosin conformational states determined by single fluorophore polarization
Muscle contraction is powered by the interaction of the molecular motor myosin with actin. With new techniques for single molecule manipulation and fluorescence detection, it is now possible to correlate, within the same molecule and in real time, conformational states and mechanical function of myosin. A spot-confocal microscope, capable of detecting single
The National Academy of Sciences.