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Mostrando 1-12 de 30 artigos, teses e dissertações.
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1. Evidência molecular de Borrelia burgdorferi sensu lato em pacientes no centro-oeste brasileiro
Resumo Este estudo promoveu a detecção de DNA de Borrelia burgdorferi sensu lato em amostras de sangue e soro de pacientes com manifestações clínicas e epidemiologia compatíveis com a doença de Lyme-símile brasileira ou síndrome de Baggio-Yoshinari. Para tanto, foi feita triagem sorológica pelos métodos de Elisa e Western blotting e a identificaç
Rev. Bras. Reumatol.. Publicado em: 2017-12
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2. Amplificação do gene flgE fornece evidência para a existência de uma borreliose brasileira
INTRODUÇÃO: Os sintomas da borreliose brasileira se assemelham às manifestações clínicas da Doença de Lyme (DL), porém, existem diferenças epidemiológicas e laboratoriais entre essas enfermidades. Primers normalmente utilizados para diagnosticar a DL não conseguiram detectar cepas de borrelia no Brasil. OBJETIVO: O objetivo desse trabalho foi iden
Revista do Instituto de Medicina Tropical de São Paulo. Publicado em: 2012-06
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3. The flgE gene of Campylobacter coli is under the control of the alternative sigma factor sigma54.
The flgE gene encoding the flagellar hook protein of Campylobacter coli VC167-T1 was cloned by immunoscreening of a genomic library constructed in lambdaZAP Express. The flgE DNA sequence was 2,553 bp in length and encoded a protein with a deduced molecular mass of 90,639 Da. The sequence had significant homology to the 5' and 3' sequences of the flgE genes
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4. The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease.
The periplasmic flagellum of Borrelia burgdorferi consists of a unipeptide flagellar filament, a hook, and a basal body. Here, we report the cloning and expression of the hook gene, flgE, of B. burgdorferi N40. The flgE gene is 1,119 nucleotides long and is located on the 950-kb linear chromosome of B. burgdorferi. The primary protein sequence of FlgE shows
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5. The Flagellar Hook Protein, FlgE, of Salmonella enterica Serovar Typhimurium Is Posttranscriptionally Regulated in Response to the Stage of Flagellar Assembly
We investigated the posttranscriptional regulation of flgE, a class 2 gene that encodes the hook subunit protein of the flagella. RNase protection assays demonstrated that the flgE gene was transcribed at comparable levels in numerous strains defective in known steps of flagellar assembly. However, Western analyses of these strains demonstrated substantial d
American Society for Microbiology.
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6. The Hook Gene (flgE) Is Expressed from the flgBCDEF Operon in Rhodobacter sphaeroides: Study of an flgE Mutant
In this work we identified the flgE gene encoding the flagellar hook protein from Rhodobacter sphaeroides. Our results show that this gene is part of a flagellar cluster that includes the genes flgB, flgC, flgD, flgE, and flgF. Two different types of mutants in the flgE gene were isolated, and both showed a Fla− phenotype, indicating the functionality of t
American Society for Microbiology.
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7. The Central, Surface-Exposed Region of the Flagellar Hook Protein FlgE of Campylobacter jejuni Shows Hypervariability among Strains
In a previous study, we observed that monoclonal antibodies raised against the hook protein FlgE of Campylobacter jejuni LIO 36, isolate 5226, bound exclusively to this strain. The aim of this study was to elucidate the molecular basis for these binding specificities. The hook protein-encoding gene flgE of C. jejuni was cloned in Escherichia coli and sequenc
American Society for Microbiology.
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8. Complementation of a Treponema denticola flgE Mutant with a Novel Coumermycin A1-Resistant T. denticola Shuttle Vector System
By using the mutated gyrB gene from a spontaneous coumermycin A1-resistant Treponema denticola, an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to coumermycin was constructed. The complete T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed into the T. denticola ATCC 33520 flgE
American Society for Microbiology.
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9. Effect of Hook Subunit Concentration on Assembly and Control of Length of the Flagellar Hook of Salmonella
The flagellar hook of Salmonella is a filamentous polymer made up of subunits of the protein FlgE. Hook assembly is terminated when the length reaches about 55 nm. After our recent study of the effect of cellular levels of the hook length control protein FliK, we have now analyzed the effect of cellular levels of FlgE itself. When FlgE was overproduced in a
American Society for Microbiology.
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10. Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.
Treponema denticola is implicated in the etiology of periodontal diseases. We now report the construction of a specific flgE mutant of T. denticola ATCC 35405 following electroporation utilizing an erythromycin resistance cassette inserted into an flgE DNA fragment. The resulting mutant displays no visible motility and lacks periplasmic flagella as would be
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11. Genetic and biochemical analysis of the flagellar hook of Treponema phagedenis.
The periplasmic flagellum of Treponema phagedenis consists of the flagellar filament and hook-basal body. We report here a characterization of the hook gene and flagellar hook of T. phagedenis, and in the process of this analysis we found evidence that the hook polypeptide is likely cross-linked in situ. A T. phagedenis genomic library was screened with a Tr
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12. Organization, transcription, and expression of the 5' region of the fla operon of Treponema phagedenis and Treponema pallidum.
A locus encoding polypeptides associated with flagellar structure and function was identified, sequenced, and characterized in Treponema phagedenis and Treponema pallidum. This locus includes homologs of the FlgD, FlgE, MotA, MOB, FliL, and FliM polypeptides found in Salmonella typhimurium and Bacillus subtilis. These polypeptides are extensively conserved b