Exou
Mostrando 1-12 de 32 artigos, teses e dissertações.
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1. Virulence factors and integrons are associated with MDR and XDR phenotypes in nosocomial strains of Pseudomonas aeruginosa in a Venezuelan university hospital
ABSTRACT Multidrug resistance (MDR), virulence and transferable elements potentiate Pseudomonas aeruginosa's role as an opportunistic pathogen creating a high risk for public health. In this study, we evaluated the possible association of multidrug resistance, virulence factors and integrons with intrahospital P. aeruginosa strains isolated from patients at
Rev. Inst. Med. trop. S. Paulo. Publicado em: 04/04/2019
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2. The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
An increased plasma concentration of von Willebrand factor (vWF) is detected in individuals with many infectious diseases and is accepted as a marker of endothelium activation and prothrombotic condition. To determine whether ExoU, a Pseudomonas aeruginosa cytotoxin with proinflammatory activity, enhances the release of vWF, microvascular endothelial cells w
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-09
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3. Efeito de ExoU na ativação de NF-κB e na secreção de IL-8 por células humanas infectadas por Pseudomonas aeruginosa / Effect of Exou on the activation of the NF-κB and the secretion of the IL-8 in human cells infected with Pseudomonas
ExoU, a cytotoxin produced by the opportunistic pathogen Pseudomonas aeruginosa that is translocated into host cell cytosol by the type three secretory system, has been associated with severity of acute infections. We have previously described the potent ExoU proinflammatory activity, which accounts for a market recruitment of neutrophils to infected tissues
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/07/2011
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4. Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa
The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups, infection localization and antimicrobial resistance. The following frequencies in all studied strains were es
Brazilian Journal of Microbiology. Publicado em: 2010-10
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5. Atividade pró-coagulante da toxina ExoU de Pseudomonas aeruginosa: efeito sobre a expressão do fator tissular em células epiteliais respiratórias / Procoagulant activity of Pseudomonas aeruginosa toxin ExoU: effect on the expression of tissue factor by airway epithelial cells
To evaluate the capacity of the P. aeruginosa toxin ExoU to induce the expression of tissue factor (TF) by epithelial respiratory cells from the BEAS-2B cell line, cells infected with the ExoU-producing PA103 bacterial strain were compared with cells infected with a mutant obtained by deletion of the exoU gene and with control non-infected cells in their i)
Publicado em: 2008
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6. ExoU contribui para a morte tardia de células endoteliais infectadas por Pseudomonas aeruginosa
Para determinar o papel de ExoU na citotoxicidade tardia de P. aeruginosa, células endoteliais (CE) foram expostas às cepas PA103, PA103deltaxoU e PA103::exsA por 1h e à gentamicina em meio de cultura. Após 24h, a viabilidade das CE infectadas com PA103 (33.7 ± 14.3%) foi inferior à de CE infectadas com PA103deltaexoU (77.7 ± 6.3%) e PA103::exsA (79.5
Brazilian Journal of Microbiology. Publicado em: 2003-11
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7. The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU
Pseudomonas aeruginosa delivers the toxin ExoU to eukaryotic cells via a type III secretion system. Intoxication with ExoU is associated with lung injury, bacterial dissemination and sepsis in animal model and human infections. To search for ExoU targets in a genetically tractable system, we used controlled expression of the toxin in Saccharomyces cerevisiae
Oxford University Press.
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8. Multiple Domains Are Required for the Toxic Activity of Pseudomonas aeruginosa ExoU
Expression of ExoU by Pseudomonas aeruginosa is correlated with acute cytotoxicity in a number of epithelial and macrophage cell lines. In vivo, ExoU is responsible for epithelial injury. The absence of a known motif or significant homology with other proteins suggests that ExoU may possess a new mechanism of toxicity. To study the intracellular effects of E
American Society for Microbiology.
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9. Identification and Characterization of SpcU, a Chaperone Required for Efficient Secretion of the ExoU Cytotoxin
In recent studies, we have shown that Pseudomonas aeruginosa strains that are acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part of the exoenzyme S regulon. The specific virulence determinant responsible for epithelial damag
American Society for Microbiology.
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10. Functional Regions of the Pseudomonas aeruginosa Cytotoxin ExoU
ExoU, a potent patatin-like phospholipase, causes rapid cell death following its injection into host cells by the Pseudomonas aeruginosa type III secretion system. To better define regions of ExoU required for cytotoxicity, transposon-based linker insertion mutagenesis followed by site-directed mutagenesis of individual residues was employed by using a Sacch
American Society for Microbiology.
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11. Pseudomonas aeruginosa ExoU, a Toxin Transported by the Type III Secretion System, Kills Saccharomyces cerevisiae
ExoU, a protein transported by the type III secretion system of Pseudomonas aeruginosa, is an important cytotoxin, though its mechanism of action is unclear. Here we show that the intracellular expression of ExoU is cytotoxic to Saccharomyces cerevisiae. Furthermore, internal amino- and carboxyl-terminal deletions confirmed that regions of ExoU previously sh
American Society for Microbiology.
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12. Acquisition of Expression of the Pseudomonas aeruginosa ExoU Cytotoxin Leads to Increased Bacterial Virulence in a Murine Model of Acute Pneumonia and Systemic Spread
Pseudomonas aeruginosa is the nosocomial bacterial pathogen most commonly isolated from the respiratory tract. Animal models of this infection are extremely valuable for studies of virulence and immunity. We thus evaluated the utility of a simple model of acute pneumonia for analyzing P. aeruginosa virulence by characterizing the course of bacterial infectio
American Society for Microbiology.