Escherichia Coli Isolation And Purification
Mostrando 1-12 de 74 artigos, teses e dissertações.
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1. Isolamento, purificação e caracterização molecular de bacteriófagos específicos para Escherichia coli causadora de mastite bovina / Isolation, purification and molecular characterization of bacteriophages specific for Escherichia coli causing bovine mastitis
A mastite bovina causa sérios prejuízos tanto ao produtor, quanto à indústria de produtos lácteos. É causada por diversos patógenos, divididos em dois grupos principais: ambientais e contagiosos. Enquanto o número de casos de mastite contagiosa vem diminuindo, os casos causados por patógenos ambientais aumentam, sendo a Escherichia coli o principal
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/06/2011
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2. Caracterização de beta-lactamases de espectro estendido e determinação de grupos filogenéticos em isolados de Escherichia coli recuperados de pacientes em um hospital universitário de São Paulo. / Characterization of extended-spectrum b-lactamases and phylogenetic groups in Escherichia coli strains recovered from patients at a university hospital in São Paulo.
Escherichia coli pode causar infecção intestinal e extra-intestinal, de origem comunitária ou hospitalar, prevalecendo como agente de infecção do trato urinário (ITU). O objetivo do presente estudo foi caracterizar a produção de b-lactamases de espectro estendido (ESBL), grupos filogenéticos, e a relação clonal em isolados cl
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/04/2011
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3. Nucleotide phosphotransferase of Escherichia coli: purification by affinity chromatography.
Improved extraction and purification procedures permit the isolation from Escherichia coli W cells of much larger quantities and of more highly purified preparations of nucleotide phosphotransferase. Of various affinity resins tested for efficiency of purification, columns of agarose/5'-AMP (AGAMP), type 3, proved the best. In this way a 300- to 450-fold pur
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4. Isolation, Affinity Purification, and Identification of Piglet Small Intestine Mucosa Receptor for Enterotoxigenic Escherichia coli K88ac+ Fimbriae
An affinity chromatography technique was utilized to isolate and purify the receptors of Escherichia coli K88ac+ fimbriae from the mucus of the small intestines of newborn piglets. Purified K88ac+ fimbriae were covalently immobilized onto a beaded agarose matrix (Sepharose 4B). The immobilized fimbriae were used for the affinity purification of the K88ac+ re
American Society for Microbiology.
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5. Serotypes of attachment pili of enterotoxigenic Escherichia coli isolated from humans.
Pili from enterotoxigenic Escherichia coli isolated from humans have been partially purified, and antisera have been prepared. These pili were initially attached to erythrocytes and then removed by thermal elution for purification. Three distinct antigenic types of pili have been identified. Antisera against these three pili types reacted with 60 of 106 (56%
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6. Isolation of a replication origin complex from Escherichia coli.
A complex consisting of replicative origin DNA and several proteins was isolated from Escherichia coli. Cells of temperature-sensitive mutants were labeled at the origin and fractionated by sucrose gradient centrifugation. A complex highly purified in origin DNA sedimented as a unique band. This complex dissociated at high concentration, above 0.2 M KCl. Upo
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7. Isolation of the Bacteriophage Lambda Receptor from Escherichia coli
A factor which inactivates the phage lambda can be extracted from Escherichia coli. This factor is a protein and is located in the outer membrane of the bacterial envelope. It is found in extracts of strains which are sensitive to phage lambda, but not in extracts of strains specifically resistant to this phage. We conclude that this factor is the lambda rec
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8. Isolation and Purification of Two Novel Streptomycete RNase Inhibitors, SaI14 and SaI20, and Cloning, Sequencing, and Expression in Escherichia coli of the Gene Coding for SaI14
Two new RNase inhibitors, SaI14 (Mr, ∼14,000) and SaI20 (Mr, ∼20,000), were isolated and purified from a Streptomyces aureofaciens strain. The gene sai14, coding for SaI14 protein, was cloned and expressed in Escherichia coli. The alignment of the deduced amino acid sequence of SaI14 with that of barstar, the RNase inhibitor from Bacillus amyloliquefacie
American Society for Microbiology.
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9. New Procedures for Purification of l-Asparaginase with High Yield from Escherichia coli
l-Asparaginase is now known to be a potent antineoplastic agent in animals and has given complete remission in some human leukemias. Extensive clinical trials of this enzyme, however, were not possible in the past because of inadequate production of this substance. We have developed practical procedures for producing l-asparaginase in yields of sufficient qu
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10. New Method for isolation of immunologically pure pili from Escherichia coli.
A new technique for purification of bacterial pili was developed and applied to Escherichia coli strains isolated from the urine of patients with symptomatic urinary tract infections. After mechanical detachment from the bacterial cells, the pili were concentrated by precipitation with ammonium sulfate, dialyzed, and solubilized in buffer containing deoxycho
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11. Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein.
We have determined the nucleotide sequence of a 1.4-kb-pair fragment of the E. coli chromosome that carries the complete rnd gene encoding RNase D, a putative tRNA processing enzyme. The coding region of rnd extends for a total of 1128 nucleotides beginning at an initiator UUG codon and terminating at a UAA codon, and encodes a 375-amino acid polypeptide of
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12. High-Level Production of Human Leptin by Fed-Batch Cultivation of Recombinant Escherichia coli and Its Purification
Human leptin is a 16-kDa (146-amino-acid) protein that is secreted from adipocytes and influences body weight homeostasis. In order to obtain high-level production of leptin, the human obese gene coding for leptin was expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. The recombinant leptin was produced as inclusion bodies in E.
American Society for Microbiology.