Enzymatic Hydrolyzes
Mostrando 1-12 de 44 artigos, teses e dissertações.
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1. Imobilização da tanase de Paecilomyces variotii e ação em reações de hidrólise e síntese / Immobilization of tannase from Pecilomyces variotii and reactions of hydrolysis and synthesis
Tannin acyl hydrolase (TAH) known as tannase (EC 3.1.1.20) is an enzyme that hydrolyzes esters of hydrolysable tannins producing gallic acid and glucose as the reaction medium is polar. However, the literature indicates their ability to produce also gallic acid esters in organic media. Our research group has isolated a fungus, Paecilomyces variotii, tannase
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/06/2012
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2. Pré-tratamento do bagaço de cana de açúcar com amônia aquosa para a produção de etanol
O objetivo deste trabalho foi avaliar a etapa de pré-tratamento do bagaço de cana de açúcar empregando amônia aquosa, um método alcalino. Amostras de bagaço (in natura e explodido a vapor não lavado) foram tratadas na proporção 1:5 (massa de bagaço seco por massa de solução de NH4OH), em escala de bancada. Ensaios preliminares foram realizados e
Publicado em: 2011
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3. Estudo da utilização de lipases no enriquecimento de oleos de sardinha e Mucor sp em acidos graxos poliinsaturados
The present work reports the study of the hydrolysis and etherification reactions catalyzed by microorganism lipases, which were isolated at the "Laboratorio de Bioquímica de Alimentos - FEA - UNICAMP", belonging to Rhizopus sp, Geotrichum sp, Aspergillus sp and the commerciallipase Candida cylindracea. The aim was the enrichment of polyunsaturated fatty ac
Publicado em: 1998
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4. Cyclic Nucleotide Phosphodiesterase Activity in Barley Seeds 12
Barley seeds (Hordeum vulgare L. cv. Himalaya) contain an enzymatic activity which catalyzes the hydrolysis of adenosine cyclic 3′: 5′-monophosphate and adenosine cyclic 2′: 3′-monophosphate. A large portion of the enzymatic activity is present in the dry seed, existing in both soluble and particulate form. Secretion of the soluble phosphodiesterase
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5. CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung.
Bombesin-like peptides are essential autocrine growth factors for many small cell carcinomas (SCCas) of the lung. Herein, we demonstrate that these malignant pulmonary neuroendocrine cells express low levels of the cell surface metalloendopeptidase CD10/neutral endopeptidase 24.11 (CD10/NEP, common acute lymphoblastic leukemia antigen) and that this enzyme h
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6. Morphological Changes in Poly-β-Hydroxybutyrate Granules Associated with Decreased Susceptibility to Enzymatic Hydrolysis
Merrick, J. M. (State University of New York, Buffalo), D. G. Lundgren, and R. M. Pfister. Morphological changes in poly-β-hydroxybutyrate granules associated with decreased susceptibility to enzymatic hydrolysis. J. Bacteriol. 89:234–239. 1965.—A complex enzyme system obtained from extracts of Rhodospirillum rubrum cells hydrolyzes poly-β-hydroxybutyr
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7. Hyperthermostable Endoglucanase from Pyrococcus horikoshii
An endoglucanase homolog from the hyperthermophilic archaeon Pyrococcus horikoshii was expressed in Escherichia coli, and its enzymatic characteristics were examined. The expressed protein was a hyperthermostable endoglucanase which hydrolyzes celluloses, including Avicel and carboxymethyl cellulose, as well as β-glucose oligomers. This enzyme is the first
American Society for Microbiology.
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8. DNase Specific for Uracil-Containing Bacteriophage DNA
A DNase from Bacillus subtilis which specifically hydrolyzes native DNA of phage PBS 1 has been purified and characterized. The mode of action of the enzyme is endonucleolytic, yielding deoxyuridine and oliogonucleotides of various sizes. The primary site of enzymatic attack is deoxyuridylic acid in the DNA. A mild nitrous acid treatment of thymine-containin
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9. Cloning of β-Primeverosidase from Tea Leaves, a Key Enzyme in Tea Aroma Formation1
A β-primeverosidase from tea (Camellia sinensis) plants is a unique disaccharide-specific glycosidase, which hydrolyzes aroma precursors of β-primeverosides (6-O-β-d-xylopyranosyl-β-d-glucopyranosides) to liberate various aroma compounds, and the enzyme is deeply concerned with the floral aroma formation in oolong tea and black tea during the manufacturi
American Society of Plant Biologists.
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10. Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia.
Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modified human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit con
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11. Identification of MpaA, an Amidase in Escherichia coli That Hydrolyzes the γ-d-Glutamyl-meso-Diaminopimelate Bond in Murein Peptides
MpaA amidase was identified in Escherichia coli by its amino acid sequence homology with the ENP1 endopeptidase from Bacillus sphaericus. The enzymatic activity of MpaA, i.e., hydrolysis of the γ-d-glutamyl-diaminopimelic acid bond in the murein tripeptide l-alanyl-γ-d-glutamyl-meso-diaminopimelic acid, was demonstrated in the cell extract of a strain expr
American Society for Microbiology.
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12. Molecular cloning, nucleotide sequencing, and expression of the Bacillus subtilis (natto) IAM1212 alpha-amylase gene, which encodes an alpha-amylase structurally similar to but enzymatically distinct from that of B. subtilis 2633.
An alpha-amylase gene of Bacillus subtilis (natto) IAM1212 was cloned in a lambda EMBL3 bacteriophage vector, and the nucleotide sequence was determined. An open reading frame encoding the alpha-amylase (AMY1212) consists of 1,431 base pairs and contains 477 amino acid residues, which is the same in size as the alpha-amylase (AMY2633) of B. subtilis 2633, an