Elispot
Mostrando 1-12 de 127 artigos, teses e dissertações.
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1. Enhancing immune effects of a DNA vaccine against kidney cancer using CD40L as an adjuvant
The use of specific combinations of antigens and adjuvant represents a promising approach for increasing the immunogenicity of DNA vaccines. In the present study, we evaluated the immunity and antitumor effects of DNA vaccines with G250 as the target antigen in a mouse model of renal cell carcinoma. We constructed two recombinant plasmids, pVAX1-G250 and pVA
Braz. J. Pharm. Sci.. Publicado em: 25/11/2019
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2. The immunogenicity and anti-tumor effects of a lung cancer DNA vaccine harboring a MUC-1 and GM-CSF fusion gene
ABSTRACT DNA vaccines have been shown to be an effective approach to induce antigen-specific cellular and humoral immunity. However, the inability of DNA vaccines to elicit strong immune responses in clinical trials limits the application of DNA vaccines. Here, we developed a new DNA vaccine based on MUC1, which has been suggested as a potential target for l
Braz. arch. biol. technol.. Publicado em: 27/06/2016
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3. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test
The interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic p
Mem. Inst. Oswaldo Cruz. Publicado em: 09/12/2014
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4. Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
BACKGROUND: Atypical spinal tuberculosis (TB) usually presents in a slowly indolent manner with nonspecific clinical presentations making the diagnosis a great challenge for physicians. New technologies for the detection of atypical spinal TB are urgently needed. The aim of this study was to assess the diagnostic value of an enzyme-linked immunospot (ELISPOT
Braz J Infect Dis. Publicado em: 2013-10
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5. Estudo da imunogenicidade das proteínas SAG1, SAG2 e SAG3 de Toxoplasma gondii expressas em vírus recombinantes e mapeamento de seus epítopos protetores
As proteínas de superfície (SAGs) presentes em taquizoitos de T. gondii encontram-se envolvidas na ativação de resposta imune contra o parasita. No presente estudo nós avaliamos a capacidade de adenovírus recombinantes codificando os genes de SAG1, SAG2 e SAG3 em induzir a ativação de resposta imune humoral e celular, além de imunidade protetora con
Publicado em: 2010
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6. O desafio da tuberculose na faixa etária pediátrica frente a novas técnicas diagnósticas
OBJETIVOS: Apresentar uma revisão atualizada sobre os novos métodos para o diagnóstico da tuberculose baseados na produção in vitro de interferon-gama (IFN-γ) por células T dos pacientes sob investigação, comparando-os com a tradicional prova tuberculínica. FONTES DOS DADOS: Revisão de literatura utilizando os bancos de dados MEDLINE e LILACS (200
Jornal de Pediatria. Publicado em: 2009-06
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7. Mapeamento de epÃtopos das proteÃnas estruturais VP1 e VP4 do vÃrus da hepatite A reconhecidos por cÃlulas T
Hepatitis A infection is found all over the world, and high prevalence is associated with poor socioeconomic conditions and diverse epidemiological patterns. The epitope mapping of hepatitis A virus (HAV) can to contribute with the development of a potentially safer vaccine against HAV and the identification of HLA binding molecules is important for both und
Publicado em: 2009
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8. Measurement of peripheral blood mononuclear cells producing IFN-Gamma in patients with tuberculosis
The type of immune response induced by tuberculosis (Th1 or Th2) and its correlation with the clinical outcome is unclear. We studied 13 patients with active tuberculosis (TBC). The peripheral blood mononuclear cells producing IFN-gamma (PBMC-IG) were measured by enzyme-linked immunospot (ELISPOT) technique. The control group had ten healthy individuals vacc
Brazilian Journal of Infectious Diseases. Publicado em: 2008-04
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9. Characterization of response immune of human cells to proteins recombinant LACK and KMP-11 of leishmania amazonensis. / Caracterização da resposta imune de células humanas às proteínas recombinantes LACK e KMP-11 de leishmania amazonensis
Vaccination represents a reasonable perspective for leishmaniasis control besides being a cost-effective approach to infectious disease control in general. This study was performed to evaluate the immune response of human cells to the Leishmania amazonensis proteins KMP-11 and LACK, both of them considered as candidate antigens for a leishmaniasis vaccine. W
Publicado em: 2008
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10. Study of the recognition of HIV-1 Gag and Nef epitopes by T lymphocytes in chronically infected HIV-1 Long-Term Non-Progressors / Estudo do reconhecimento de epitopos das proteínas Gag e Nef do HIV-1 por linfócitos T em indivíduos cronicamente infectados pelo HIV-1 não progressores por longo tempo
Os linfócitos T têm um papel central no controle da infecção pelo HIV-1. As respostas mediadas por esses linfócitos contra epitopos do HIV-1 restritos a moléculas HLA de classe I podem estar associadas à proteção natural em indivíduos LTNP. Relatos sugerem que determinados alelos HLA apresentamse mais representados entre os LTNP. Para avaliar esses
Publicado em: 2008
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11. Evaluation of the effect of TNF-alpha inhibitors in the in vitro immune response to Mycobacterium tuberculosis antigens in patients with psoriasis / Avaliação do efeito do bloqueio de Fator de Necrose Tumoral alfa (TNF-) na resposta imune in vitro aos antígenos de Mycobacterium tuberculosis em pacientes com psoríase
O Fator de Necrose Tumoral-alfa (TNF-alfa) possui um importante papel na imunopatogênese da psoríase e agentes biológicos, como os inibidores de TNF-alfa, têm apresentado bons resultados no tratamento desta. No entanto, estes agentes foram associados ao aumento de casos de reativação de tuberculose entre os pacientes que os utilizaram. Este estudo foi
Publicado em: 2008
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12. Adordagem vacinal bivalente baseada na fusão genética de seqüência derivada da proteína circunsporozoíta (CS) de Plasmodium yoelii com a flagelina FliCd de Salmonella enterica / Bivalent vaccines approaches based in genetic fusion of sequence derived of the circumsporozoíta (CS) protein de Plasmodium yoelii with FliCd flagellin of Salmonella enterica.
A busca de adjuvantes que estimulem de forma eficiente à resposta imune celular representa uma importante contribuição para a pesquisa de vacinas. O principal objetivo deste trabalho foi a avaliação do efeito imunoestimulador de flagelinas expressas por linhagens de Salmonella sp., particularmente na ativação de respostas mediadas por células T CD8+,
Publicado em: 2007