Double Accounting
Mostrando 13-23 de 23 artigos, teses e dissertações.
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13. Calculation of hydrodynamic properties of small nucleic acids from their atomic structure
Hydrodynamic properties (translational diffusion, sedimentation coefficients and correlation times) of short B-DNA oligonucleotides are calculated from the atomic-level structure using a bead modeling procedure in which each non-hydrogen atom is represented by a bead. Using available experimental data of hydrodynamic properties for several oligonucleotides,
Oxford University Press.
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14. The S-layer of Caulobacter crescentus: three-dimensional image reconstruction and structure analysis by electron microscopy.
The regular surface protein structure (S-layer) of Caulobacter crescentus was analyzed by electron microscopy and three-dimensional image reconstruction to a resolution of 2 nm. Projections showed that the S-layer is an array of ring structures, each composed of six subunits that are arranged on a lattice with p6 symmetry. Three-dimensional reconstructions s
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15. Productive and Nonproductive Complexes of Ku and DNA-Dependent Protein Kinase at DNA Termini
DNA-dependent protein kinase (DNA-PK) is the only eukaryotic protein kinase known to be specifically activated by double-stranded DNA (dsDNA) termini, accounting for its importance in repair of dsDNA breaks and its role in physiologic processes involving dsDNA breaks, such as V(D)J recombination. In this study we conducted kinase and binding analyses using D
American Society for Microbiology.
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16. Activation of natural killer T cells in NZB/W mice induces Th1-type immune responses exacerbating lupus
In vivo treatment of mice with the natural killer T (NKT) cell ligand, α-galactosylceramide (αGalCer), ameliorates autoimmune diabetes and experimental autoimmune encephalomyelitis (EAE) by shifting pathogenic Th1-type immune responses to nonpathogenic Th2-type responses. In the current study, in vivo activation of NKT cells in adult NZB/W mice by multiple
American Society for Clinical Investigation.
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17. Pharmacokinetics and tolerability of teicoplanin in healthy volunteers after single increasing doses.
In this double-blind, randomized study, five healthy subjects per group received doses of 15, 20, or 25 mg of teicoplanin per kg of body weight, and one subject per group received a 0.9% NaCl placebo as single intravenous infusion over 30 min. Serial blood samples and urine were collected for 13 days postadministration, and concentrations of teicoplanin were
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18. Assembly of RecA-like recombinases: Distinct roles for mediator proteins in mitosis and meiosis
Members of the RecA family of recombinases from bacteriophage T4, Escherichia coli, yeast, and higher eukaryotes function in recombination as higher-order oligomers assembled on tracts of single-strand DNA (ssDNA). Biochemical studies have shown that assembly of recombinase involves accessory factors. These studies have identified a class of proteins, called
The National Academy of Sciences.
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19. Transport of sodium, chloride, and taurocholate by cultured rat hepatocytes
Transport of sodium, chloride, and taurocholate was studied in primary cultures of adult rat hepatocytes incubated in a balanced electrolyte solution containing 150 mM NaCl, various concentrations of taurocholate, and 22Na, 36Cl, [3H]taurocholate, and 3-O-[3H]methyl-D-glucose. Lithium chloride, choline chloride, or Na2SO4 and mannitol were substituted isoton
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20. A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52.
To understand the mechanisms involved in homologous recombination, we have performed a search for Saccharomyces cerevisiae mutants unable to carry out plasmid-to-chromosome gene conversion. For this purpose, we have developed a colony color assay in which recombination is induced by the controlled delivery of double-strand breaks (DSBs). Recombination occurs
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21. The Golgi and Endoplasmic Reticulum Remain Independent during Mitosis in HeLa Cells
Partitioning of the mammalian Golgi apparatus during cell division involves disassembly at M-phase. Despite the importance of the disassembly/reassembly pathway in Golgi biogenesis, it remains unclear whether mitotic Golgi breakdown in vivo proceeds by direct vesiculation or involves fusion with the endoplasmic reticulum (ER). To test whether mitotic Go
The American Society for Cell Biology.
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22. Molecular mechanical studies of DNA flexibility: Coupled backbone torsion angles and base-pair openings
Molecular mechanics studies have been carried out on “B-DNA-like” structures of [d(C-G-C-G-A-A-T-T-C-G-C-G)]2 and [d(A)]12·[d(T)]12. Each of the backbone torsion angles (ψ, φ, ω, ω′, φ′) has been “forced” to alternative values from the normal B-DNA values (g+, t, g-, g-, t conformations). Compensating torsion angle changes preserve most of
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23. Transient introduction of a foreign gene into healing rat patellar ligament.
We investigated the in vivo introduction of a reporter gene into healing rat patellar ligaments using the hemagglutinating virus of Japan (HVJ)-liposome-mediated gene transfer method. The mid-portion of the medial half of the patellar ligament was cut transversely with a scalpel in 14-wk-old male Wistar rats. A HVJ-liposome suspension containing beta-galacto